Browsing by Author "Kang S"

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    Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats
    (MDPI (Basel, Switzerland), 2021-09-25) He Y; Liu X; De J; Kang S; Munday JS; Bleach E
    This experiment compared secondary hair follicles (SFs) in Tibetan cashmere goats from two different steppes that were at different altitudes and had different temperatures. Twenty-four 2-year-old goats were studied. Twelve goats were from Rikaze in Tibet which is at an altitude of above 5000 m with an average temperature of 0 °C. The other 12 studied goats were from Huan County of Gansu Province which is around 2000 m above sea level with an average temperature of 9.2 °C. The structural features of SFs were assessed using light microscopy and transmission electron microscopy. The presence of HIF-1a, HIF-2a, HIF-3a, HSP27, and HOXC13 proteins was studied using immunohistochemistry and immunofluorescence. Light and electron microscopy revealed that the SFs of the Tibetan cashmere goats that lived in the Rikaze Steppe were in the proanagen stage in May. However, the SFs of the goats from the lower warmer Huan County were in the anagen stage at the same time. Immunohistochemistry revealed intense immunostaining for HIF-1a protein in the inner root sheath (IRS) and hair shaft (HS); immunostaining against HIF-2a in the outer root sheath (ORS) and IRS; HIF-3a protein immunostaining in the ORS; HSP27 immunostaining in the ORS, IRS, and HS; and HOXC13 immunostaining in the ORS and HS. HIF-1a protein expression in the IRS and HS was higher than the expression in the ORS (p < 0.05) while the expression of HIF-2a protein was higher in the ORS and IRS than the HS (p < 0.05). The expression of HIF-3a protein was higher in the ORS than in the IRS (p < 0.05). Expression of HOXC13 protein was higher in the ORS than in the IRS and HS (p < 0.05). Immunostaining of HIF-1a, HIF-2a, and HSP27 protein was significantly higher in SFs from cashmere goats from Rikaze than in goats from Huan (p < 0.05). In contrast, HOX13 protein immunostaining was significantly higher in cashmere goats from Huan than from Rikaze (p < 0.05). Significant differences were observed in the SFs of cashmere goats from two locations that differ in altitude and temperature. This suggests the differences in the secondary hair follicles could be due to the hypoxia and lower temperatures experienced by the goats in Rikaze. These results are useful in understanding how altitude and temperature influence SF development. Hair produced by the SFs are used for down fiber. Therefore, understanding of the factors that influence SF development will allow the production and harvest of these valuable fibers to be maximized.
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    Transverse-momentum and pseudorapidity distributions of charged hadrons in pp collisions at square root of s = 7 TeV.
    (AMER PHYSICAL SOC, 9/07/2010) Khachatryan V; Sirunyan AM; Tumasyan A; Adam W; Bergauer T; Dragicevic M; Erö J; Fabjan C; Friedl M; Frühwirth R; Ghete VM; Hammer J; Hänsel S; Hoch M; Hörmann N; Hrubec J; Jeitler M; Kasieczka G; Kiesenhofer W; Krammer M; Liko D; Mikulec I; Pernicka M; Rohringer H; Schöfbeck R; Strauss J; Taurok A; Teischinger F; Waltenberger W; Walzel G; Widl E; Wulz C-E; Mossolov V; Shumeiko N; Suarez Gonzalez J; Benucci L; Ceard L; De Wolf EA; Hashemi M; Janssen X; Maes T; Mucibello L; Ochesanu S; Roland B; Rougny R; Selvaggi M; Van Haevermaet H; Van Mechelen P; Van Remortel N; Adler V; Beauceron S; Blyweert S; D'Hondt J; Devroede O; Kalogeropoulos A; Maes J; Maes M; Tavernier S; Van Doninck W; Van Mulders P; Villella I; Chabert EC; Charaf O; Clerbaux B; De Lentdecker G; Dero V; Gay APR; Hammad GH; Marage PE; Vander Velde C; Vanlaer P; Wickens J; Costantini S; Grunewald M; Klein B; Marinov A; Ryckbosch D; Thyssen F; Tytgat M; Vanelderen L; Verwilligen P; Walsh S; 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CMS Collaboration
    Charged-hadron transverse-momentum and pseudorapidity distributions in proton-proton collisions at square root of s = 7  TeV are measured with the inner tracking system of the CMS detector at the LHC. The charged-hadron yield is obtained by counting the number of reconstructed hits, hit pairs, and fully reconstructed charged-particle tracks. The combination of the three methods gives a charged-particle multiplicity per unit of pseudorapidity dN(ch)/dη|(|η|<0.5) = 5.78 ± 0.01(stat) ± 0.23(syst) for non-single-diffractive events, higher than predicted by commonly used models. The relative increase in charged-particle multiplicity from square root of s = 0.9 to 7 TeV is [66.1 ± 1.0(stat) ± 4.2(syst)]%. The mean transverse momentum is measured to be 0.545 ± 0.005(stat) ± 0.015(syst)  GeV/c. The results are compared with similar measurements at lower energies.