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    Genomic architecture of resistance to latania scale (H. lataniae) in kiwifruit (A. chinensis var. chinensis)
    (BioMed Central Ltd, 2023-10-31) Flay C; Tahir J; Hilario E; Fraser L; Stannard K; Symonds V; Datson P
    BACKGROUND: Latania scale (Hemiberlesia lataniae Signoret) is an armoured scale insect known to cause damage to kiwifruit plants and fruit, which ultimately reduces crop values and creates post-harvest export and quarantine issues. Resistance to H. lataniae does exist in some commercial cultivars of kiwifruit. However, some of the commercial cultivars bred in New Zealand have not inherited alleles for resistance to H. lataniae carried by their parents. To elucidate the architecture of resistance in the parents and develop molecular markers to assist breeding, these experiments analysed the inheritance of resistance to H. lataniae from families related to commercial cultivars. RESULTS: The first experiment identified a 15.97 Mb genomic region of interest for resistance to H. lataniae in rtGBS data of 3.23 to 19.20 Mb on chromosome 10. A larger population was then QTL mapped, which confirmed the region of interest as the sole locus contributing to H. lataniae resistance. inDel markers mapping the region of low recombination under the QTL peak further narrowed the region associated with H. lataniae resistance to a 5.73 Mb region. CONCLUSIONS: The kiwifruit populations and genomic methods used in this study identify the same non-recombinant region of chromosome 10 which confers resistance of A. chinensis var. chinensis to H. lataniae. The markers developed to target the H. lataniae resistance loci will reduce the amount of costly and time-consuming phenotyping required for breeding H. lataniae scale resistance into new kiwifruit cultivars.
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    Non-destructive firmness assessment of ‘SunGold’ kiwifruit a three-year study
    (Taylor and Francis Group on behalf of the Royal Society of New Zealand, 2024-02-14) Sneddon T; Rivera S; Li M; Heyes J; East A; Golding J
    Kiwifruit (Actinidia chinensis var. chinensis) firmness is routinely measured in a destructive manner for decision-making purposes. Thus, a population’s quality is inferred by measuring a sample from that population. Consequently, studies have investigated non-destructive techniques for measuring fruit firmness. However, most of these studies have been restricted to a single season or focused on performance over long-term storage. This work compared non-destructive compression (1 mm deformation) and acoustic stiffness with flesh firmness measured with a penetrometer across three seasons. ‘SunGold’ kiwifruit were harvested from 11, 9 and 3 orchards on multiple occasions in 2020, 2021 and 2022, respectively. Kiwifruit was freighted to Palmerston North and assessed on arrival. Thirty fruit per orchard were measured on lab arrival, whilst 24 fruit per orchard were stored for two weeks at 0°C prior to assessment. The non-destructive methods had a strong (r2 > 0.89–0.92) segmented correlation with flesh firmness (0.52–10 kgf). Flesh firmness could be adequately estimated with the non-destructive methods within a season. However, segmented regression performance was reduced when predicting for a season outside of the training population. Nonetheless, these non-destructive methods may be useful for estimating flesh firmness at harvest and after short-term storage (2 weeks at 0 °C).