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dc.contributor.authorCornuault, VRGen_US
dc.contributor.authorKnox, JPen_US
dc.date.available2014-04-20en_US
dc.date.issued2014-04-20en_US
dc.identifier.citation2014en_US
dc.description.abstractSandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a conventional sandwich ELISA protocol uses a carbohydrate-binding module (CBM), a small protein domain found in some carbohydrate catalysing or activating enzymes, and rat monoclonal antibodies (mAbs) which can be combined in the same ELISA plate without risk of cross reaction; the secondary anti-rat HRP antibody being only able to bind to the rat mAb and not the CBM. This protocol was developed and modified in the Prof. J. Paul Knox lab at the University of Leedsen_US
dc.relation.urihttps://bio-protocol.org/e1106en_US
dc.rights2014 The Authors; exclusive licensee Bio-protocol LLC.en_US
dc.subjectELISAen_US
dc.subjectPolysaccharideen_US
dc.subjectCarbohydrateen_US
dc.subjectPlant biochemistryen_US
dc.titleSandwich enzyme-linked immunosorbent assay (ELISA) analysis of plant cell wall glycan connectionsen_US
dc.typeInternet Publication
dc.identifier.doi10.21769/BioProtoc.1106en_US
dc.identifier.elements-id403287
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
dc.identifier.harvestedMassey_Dark
pubs.notesNot knownen_US


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