The physiology and control of crown bud formation and development in gentians : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Plant Physiology at Massey University, Palmerston North, New Zealand
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Date
2012
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Massey University
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Abstract
The spread in time to flower harvest maturity and an inability to produce an adequate
number of crown buds during crop establishment, were perceived as problems by
commercial growers of gentians for cut flowers. By identifying and investigating plant
and environment related factors that influence growth and development, the current
thesis aimed to investigate both the causes of these problems and identify potential
solutions.
Crown buds which appeared during the previous growth cycle were the source of floral
shoot production and, in both true seedlings and clonally propagated plants, these buds
typically originated on the transition zone between shoots and roots. When formed on
the transition zone the first and, as a consequence, apical bud of the crown bud cluster,
originated adventitiously. The individual crown buds which subsequently initiated
within the cluster developed as axillary buds from that apical bud. As evident from the
appearance of two to five times more crown buds and shoots, and crown bud clusters,
the naturally occurring short photoperiodic regime promoted the crown bud formation
process, compared to a long photoperiodic regime. With the application of Ethephon, or
Ethephon followed by either Thidiazuron or Paclobutrazol, it was possible to achieve a
similar increase in the number of crown buds under the long photoperiodic regime. In
contrast to the strong photoperiodic response, exposure to the cooler temperatures
experienced outside did not influence the crown bud formation process.
Once formed, crown buds were under different types and degrees of dormancy (i.e.
endo, para or eco). Dependent on both the developmental stage of plants and the
hierarchical position of buds within a crown bud cluster, shoot emergence and
subsequent development to flowering varied in the quantity, quality of shoots, and
timing in response to dormancy breaking treatments (chilling, gibberellic acid (GA3), or
clipping), Hence when applied prior to shoot emergence, application of chilling to
plants of the cultivar ‘Diva’ resulted in an eight-fold increase in the number of floral
shoots, along with a 83 day reduction in the spread in time to harvest maturity. The
requirement of chilling could be substituted by GA3. In contrast, when applied
following the start of shoot emergence, only application of GA3 was effective in
increasing shoot emergence and narrowing the spread in time to harvest maturity.Within a bud cluster, a positive relationship between duration to harvest maturity and a
negative relationship with floral shoot length was evident with hierarchical position of
the crown bud from proximal to the distal end. This influence of hierarchical position
was most evident following application of chilling or GA3, potentially explaining up to
28 days spread in duration to harvest maturity in ‘Diva’. While the aforementioned
factors related to shoot emergence, development and hierarchical position contribute to
the sources of variability within a plant or an individual cluster, both the sources and
extent of variation in time to harvest maturity, varied within cultivars, with wider spread
in time to harvest maturity in ‘Starlet’ (41 days) than ‘Diva’ (35 days) and ‘Spotlight’
(29 days).
The present study has increased the understanding of factors contributing to growth and
development of gentians as an ornamental crop. Treatments have been developed to
allow manipulation of the formation of crown buds, subsequent emergence and
compactness of flowering duration.
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Keywords
Gentians, Flowering time, Crown bud formation, Floriculture, Plant physiology