Copyright is owned by the author of this thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the author. Feeding and Dietary Practices of New Zealand Infants: An Observational Study A thesis presented in the partial fulfilment of the requirements for the degree of Doctor of Philosophy In Nutritional Science at Massey University, Albany New Zealand Kimberley Jane Brown 2023 I ABSTRACT Background: Nutrition and early-life feeding practices have short and long-term impacts on the quality and longevity of life. The importance of optimal nutrition during infancy is recognised worldwide and evidence-based infant feeding recommendations have been developed to promote infant health, growth, development, and the establishment of healthy eating behaviours. Currently, there is limited evidence on infant nutrition and feeding practices in New Zealand (NZ), with gaps in our knowledge about what infants are being fed, adherence to the Ministry of Health (MoH) ‘Healthy Eating Guidelines for New Zealand Babies and Toddlers (0–2 years old)’, and the prevalence of concerning feeding behaviours (CFB). Aim: To 1) investigate the contributions that key foods and food groups make to the dietary intake of NZ infants, 2) investigate infant adherence to key dietary indicators as recommended by the MoH’s ‘Healthy Eating Guidelines for New Zealand Babies and Toddlers (0–2 years old)’, and 3) determine the prevalence of parent-reported concerning infant feeding behaviours and associated demographic characteristics and feeding practices of NZ infants between 7.0 and 10.0 months of age. Methods: The observational First Foods NZ (FFNZ) study recruited 625 ethnically diverse infants (aged 7.0 to 10.0 months) living in Auckland and Dunedin between July 2020 and February 2022. Caregivers who were 16 years or older, spoke English, and had not recently participated in a nutritional intervention that may have influenced their infant’s diet were invited to attend two study visits. Appointments were conducted in the participant’s home, available research centre, or via Zoom (during Covid19 restrictions for second appointment only) and included two 24-hour diet recalls and demographic and feeding questionnaires. Diet recall data were analysed through FoodWorks (Version 10, Xyris Software, Australia) using the NZ Food Composition Database FOODfiles™ 2018 Version 01, and foods were allocated food and food group codes using the FFNZ coding system. Counts of foods and food groups consumed were analysed for at least one and both diet recall days, where available. Key indicators from the MoH’s ‘Healthy Eating Guidelines for New Zealand Babies and Toddlers (0–2 years old)’ that were measurable from FFNZ data and applied to those aged 7.0 to 10.0 months were extracted from questionnaire data, or where stated from 24-hour recalls. Recommendations analysed included exclusive breastfeeding to ‘around’ six months of age (defined as ‘5 months’ or ‘6 months’, being the age when something other than breast milk, i.e. either another drink, or solid foods, was first introduced); current breastfeeding; the introduction of solid foods ‘around’ six months of age” (defined II as ‘5 months’ or ‘6 months’ when the first solid food was introduced); the introduction of puréed foods and spoon-feeding when starting solid foods; offering of iron-rich foods (meat, poultry, fish, seafood, and iron fortified infant cereals), vegetables, and fruit as first foods; the daily offering of MoH food groups (24-hour recall data; vegetables, fruit, grain foods, milk and milk products, and meat and protein-rich foods); no salt and sugar added to meals (specific 24-hour recall question); avoidance of inappropriate drinks (specific 24-hour recall question; beverages other than breast milk, formula, or water such as cow’s milk as a drink, other milk, juice, soft drinks, tea, and alcohol); and use of self- feeding when developmentally appropriate. Logistic regression was then used to estimate odds ratios, 95% confidence intervals, and p-values for associated sociodemographic characteristics and key indicators. After their second appointment, caregivers were emailed a final questionnaire, which included the Paediatric Eating Assessment Tool (PediEAT). Feeding behaviours were categorised according to the total PediEAT and subscale scores (physiologic symptoms, problematic mealtime behaviours, selective/restrictive eating, and oral processing). Scores were categorised as ‘concern’ and ‘no concern’ using the PediEAT scoring system. Unpaired t-tests and the chi-squared tests determined associations between PediEAT scores and infant and caregiver sociodemographic characteristics. Logistic regression, adjusted by infant age and deprivation, determined associations between PediEAT scores and food groups consumed during both diet recalls. Data were analysed using Stata software (StataCorp, Texas) and Microsoft Excel (version 16.66). Results: Written consent was obtained from 625 caregivers. Data from the demographic and feeding questionnaire were available from all infants (n=625). All caregivers completed at least one diet recall. A second diet recall was available from 614 infants. PediEAT results were available for 554 term infants. Most infants consumed vegetables (96.2% of infants), fruit (91.8%), grain foods (90.4%), milk and milk products (64.0%), and meat and protein-rich foods (84.3%) at least once during the two 24-hour diet recall days. Commercial infant foods (CIF) were consumed by 78.1% and discretionary foods by 56.3% of infants at least once. The proportion of infants who consumed vegetables (63.2%), fruit (53.9%), grain foods (49.5%), milk and milk products (38.6%), meat and protein-rich foods (31.8%), CIF (41.8%), and discretionary foods (16.1%) on both diet recall days was lower. Overall, only 6.5% of infants met guidelines for the daily consumption of the MoH food groups. The ten most common foods consumed were carrot, banana, bread, brassicas, kumara, extruded commercial infant snacks, pumpkin, apple, potato, and commercial infant pouches. III Breastfeeding was initiated by 97.2% of mothers, and 37.8% of infants were exclusively breastfed to around six months. At the time of participation, 66.2% of infants were breastfed. Most infants met guidelines for introducing solid foods, including the age of introduction (75.4%), offering iron-rich foods as first foods (88.3%), providing puréed textures (80.3%) and spoon-feeding (74.1%). Self-feeding at the time of participation was common (86.9%). Most met guidelines for avoiding inappropriate beverages (93.9%) and adding salt (76.5%) and sugar (90.6%) to foods. Typically, infants with caregivers who were younger, higher educated, not currently working, primiparous, and living in low deprivation were more likely to meet the guidelines. Feeding behaviour scores were higher than PediEAT norm-reference values and 17.3% of infants were categorised with ‘concern’ feeding behaviours. ‘Concern’ scores were highest for selective/restrictive eating (29.2%), problematic mealtime behaviours (21.5%), and physiologic symptoms (13.7%) subscales. Mothers who were primiparous and highly educated caregivers were more likely to report ‘concern’ total PediEAT scores. Primiparous mothers and caregivers with higher education, that did not use early child education centres, and had infants of NZ Asian infant ethnicity were more likely to report problematic mealtime behaviours. Infants characterised as of ‘concern’ had significantly lower odds of consuming ‘vegetables’ and ‘meat and protein-rich foods’ and were more likely to consume ‘CIF’. Infants with problematic mealtime behaviours had a lower odds of consuming ‘discretionary foods’ and were more likely to be still breastfeeding. Conclusion: This research provides evidence on infant food and food group intake, adherence to key MoH infant feeding guidelines, and the prevalence of parent-reported infant feeding behaviours and associated demographic characteristics and feeding practices from an ethnically diverse group of NZ infants from Auckland and Dunedin. Infants were shown to consume a range of foods within the MoH food groups at least once during the study. However, only 6.5% of infants consumed all five food groups during both recalls. Grain foods, milk and milk products, and meat and protein-rich foods were the least commonly consumed food groups when investigating those consumed on both recall days, increasing the risk of nutritional deficiencies. Most infants met guidelines for introducing solid foods and avoiding inappropriate beverages and adding salt and sugar to meals, although the prevalence of exclusive breastfeeding to ‘around’ six months, however, continues to be low, particularly for first-time mothers. As seen previously, sociodemographic characteristics were also associated with adherence, identifying key groups (primiparous mothers, lower educated caregivers, those living with multiple children, and those living in areas of high deprivation) that require additional support. Finally, CFB were prevalent in IV our study, with higher scores reported by primiparous mothers and caregivers who were highly educated. Infants with CFB were less likely to meet recommendations for ‘vegetables’ and ‘meat and protein-rich foods’ and were more likely to consume ‘CIF’. Further investigation is required to understand parental perceptions of feeding behaviours and the nutritional implications of CFB. Further research will determine the nutritional implications of not meeting the MoH food group guidelines during complementary feeding, investigate what support parents need in NZ to improve adherence to the MoH breastfeeding and food group recommendations, and the impact of CFB on nutrient intake. V ACKNOWLEDGEMENTS It has been a privilege to be a part of this study and to have been awarded the Health Research Council of New Zealand doctoral scholarship. I would love to take this opportunity to express my gratitude to all of those involved in this research. No matter your role, this research would not be possible without your contribution. Firstly, I would like to thank our caregivers and infants who participated in the study. To connect with you all during such a special time of your lives was an invaluable experience. Thank you for welcoming us into your lives and I wish you all the best for your journeys to come. To my academic supervisors, Cathryn Conlon, Pamela von Hurst, and Kathryn Beck, thank you for your ongoing guidance and support during the past three years. Without your critique and expertise, the development and evolution of this thesis would not have been possible. To Anne-Louise Heath and Rachel Taylor, thank-you for promoting the need for further infant research in New Zealand and providing the opportunity to be part of the First Foods New Zealand Team. Working with you both has been an honour. To my fellow Auckland First and Young Foods New Zealand PhD candidates, Maria Casale, Rio Monzales, and Emily Jones, working with you for the past three years has been a fantastic experience. Thank you all for your dedication to the study. You are inspiring individuals, and I look forward to seeing where your journey takes you next. To Rebecca Paul and Jenny McArthur, thank you for your indispensable project management during data collection. The day-to-day running of data collection would not have been possible without you both. To the Auckland research assistants, Andrea Wei, Emily Shoesmith, and Darrian Holten, data collection would not have been the same without you. Thank you for your contribution to our study. To Lizzie Jones, Ioanna Katiforis, Andrea Wei, Neve McLean, Rebecca Paul, and Liz Fleming, thank you for your time spent during FoodWorks data entry and quality checking. Working with you all was a great experience. To Ioanna Katiforis and Liz Fleming, thank you for your assistance in developing and implementing the First Foods New Zealand food coding system – a mammoth task that has allowed insight into crucial areas of infant nutrition in New Zealand. To Jillian Haszard, thank you for your statistical support and patience during data analysis. I have learnt a great deal from you. To the remaining University of Otago and Massey University teams, thank you for your invaluable input into the study. It was a pleasure working with you all. And finally, to my friends and family, thank you for your ongoing love and support. Your continual encouragement has allowed me to achieve various career milestones during the past three years. To my parents, Jenny and Hamish, I send my deepest appreciation for your continued support and encouragement. I could not have achieved this work without you. VI TABLE OF CONTENTS ABSTRACT ..................................................................................................................................................................... I ACKNOWLEDGEMENTS ............................................................................................................................................. V TABLE OF CONTENTS ................................................................................................................................................ VI LIST OF TABLES ........................................................................................................................................................... IX LIST OF FIGURES ......................................................................................................................................................... XI LIST OF ABBREVIATIONS AND SYMBOLS ................................................................................................................ XII LIST OF PAPERS AND CONFERENCE PRESENTATIONS .......................................................................................... XIV CHAPTER 1: AN INTRODUCTION TO THIS RESEARCH ............................................................................................. 1 1.1. INTRODUCTION AND JUSTIFICATION FOR THE STUDY ............................................................................... 2 1.2. THESIS AIMS .................................................................................................................................................. 7 1.3. THESIS OBJECTIVES ....................................................................................................................................... 7 1.4. STUDY HYPOTHESES ..................................................................................................................................... 8 1.5. THESIS STRUCTURE ....................................................................................................................................... 8 1.6. RESEARCHER CONTRIBUTIONS .................................................................................................................... 9 1.7. REFERENCES ................................................................................................................................................ 13 CHAPTER 2: A REVIEW OF THE CURRENT LITERATURE ........................................................................................ 22 2.1. INFANT FEEDING GUIDELINES ..................................................................................................................... 23 2.1.1. A GLIMPSE INTO THE HISTORY OF INFANT NUTRITION ............................................................................ 23 2.1.2. THE NEW ZEALAND MINISTRY OF HEALTH INFANT FEEDING GUIDELINES .............................................. 27 2.1.3. COMPARING THE NEW ZEALAND INFANT FEEDING GUIDELINES TO GLOBAL GUIDELINES AND THOSE FROM HIGH INCOME COUNTRIES ........................................................................................................................ 33 2.2. ASSESSING THE DIETARY INTAKE OF INFANTS .......................................................................................... 50 2.2.1. ADHERENCE TO INFANT FEEDING GUIDELINES IN HIGH-INCOME COUNTRIES ....................................... 51 2.2.2. FACTORS ASSOCIATED WITH INFANT DIETARY INTAKE IN HIGH-INCOME COUNTRIES .......................... 57 2.3. FEEDING BEHAVIOURS ............................................................................................................................... 61 2.3.1. MEASURING FEEDING BEHAVIOURS ......................................................................................................... 62 VII 2.3.2. INFLUENCES ON FEEDING BEHAVIOURS ................................................................................................... 68 2.3.3. THE RELATIONSHIP BETWEEN FEEDING BEHAVIOURS AND DIETARY INTAKE ......................................... 69 2.4. CONCLUDING STATEMENT ......................................................................................................................... 70 2.5. REFERENCES ................................................................................................................................................ 72 CHAPTER 3: CONTRIBUTIONS OF KEY FOODS AND FOOD GROUPS TO THE DIETARY INTAKE OF INFANTS IN THE FIRST CFOODS NEW ZEALAND STUDY ........................................................................................................... 101 3.1. ABSTRACT ................................................................................................................................................. 102 3.2. INTRODUCTION ........................................................................................................................................ 103 3.3. METHODS .................................................................................................................................................. 105 3.4. RESULTS .................................................................................................................................................... 109 3.5. DISCUSSION .............................................................................................................................................. 117 3.6. CONCLUSION ............................................................................................................................................ 123 3.7. REFERENCES .............................................................................................................................................. 124 CHAPTER 4: ADHERENCE TO INFANT FEEDING GUIDELINES IN THE FIRST FOODS NEW ZEALAND STUDY ...... 128 4.1. ABSTRACT ................................................................................................................................................. 129 4.2. INTRODUCTION ........................................................................................................................................ 130 4.3. METHODS .................................................................................................................................................. 133 4.4. RESULTS .................................................................................................................................................... 141 4.5. DISCUSSION .............................................................................................................................................. 154 4.6. CONCLUSION ............................................................................................................................................ 159 4.7. REFERENCES .............................................................................................................................................. 161 CHAPTER 5: PARENT-REPORTED FEEDING BEHAVIOURS OF NEW ZEALAND INFANTS AND ASSOCIATIONS WITH DEMOGRAPHIC CHARACTERISTICS AND FEEDING PRACTICES IN THE FIRST FOODS NEW ZEALAND STUDY………………………………………………………………………………………………………………………………………………………………167 5.1. ABSTRACT ................................................................................................................................................. 168 5.2. INTRODUCTION ........................................................................................................................................ 169 5.3. METHODS .................................................................................................................................................. 171 5.4. RESULTS .................................................................................................................................................... 174 5.5. DISCUSSION .............................................................................................................................................. 182 VIII 5.6. CONCLUSION ............................................................................................................................................ 187 5.7. REFERENCES .............................................................................................................................................. 188 CHAPTER 6: CONCLUSIONS AND FUTURE RECOMMENDATIONS ....................................................................... 196 6.1. STUDY SUMMARY AND ACHIEVEMENT OF AIMS AND OBJECTIVES ....................................................... 197 6.2. STRENGTHS AND LIMITATIONS ................................................................................................................ 202 6.3. CONCLUDING REMARKS ........................................................................................................................... 203 6.4. RECOMMENDATIONS FOR FUTURE STUDIES .......................................................................................... 204 6.5. REFERENCES .............................................................................................................................................. 205 APPENDICES ............................................................................................................................................................ 211 APPENDIX 1: SEARCH STRATEGY ............................................................................................................................ 211 APPENDIX 2: STATEMENT OF CONTRIBUTION FOR MANUSCRIPTS ...................................................................... 213 APPENDIX 3: PUBLISHED FIRST FOODS NEW ZEALAND STUDY PROTOCOL PAPER .............................................. 216 APPENDIX 4: STUDY POSTERS ................................................................................................................................. 224 APPENDIX 5: STUDY INFORMATION SHEET ............................................................................................................ 226 APPENDIX 6: STUDY CONSENT FORM .................................................................................................................... 228 APPENDIX 7: 24-HOUR DIET RECALL STANDARD OPERATING PROCEDURE ......................................................... 231 APPENDIX 8: 24-HOUR DIET RECALL TEMPLATE .................................................................................................... 237 APPENDIX 9: FOODS FED BY OTHER ADULTS (FFOA) FORM .................................................................................. 239 APPENDIX 10: 24-HOUR DIET RECALL PROMPTS ................................................................................................... 242 APPENDIX 11: 24-HOUR DIET RECALL GRID SHEETS .............................................................................................. 246 APPENDIX 12: EXAMPLES OF FOOD AND FOOD GROUP CODING ......................................................................... 248 APPENDIX 13: MAIN QUESTIONNAIRE ................................................................................................................... 252 APPENDIX 14: FINAL QUESTIONNAIRE (PEDIEAT) .................................................................................................. 287 APPENDIX 15: NUTRITION SOCIETY CONFERENCE ABSTRACT .............................................................................. 296 IX LIST OF TABLES Chapter 1 Table 1.1: Candidate research contribution ................................................................................................... 11 Chapter 2 Table 2.1: Comparison of the 2021 and 2008 New Zealand Ministry of Health Infant Feeding Guidelines ......... 28 Table 2.2: Infant feeding guidelines identified and used to compare recommendations in high-income countries .................................................................................................................................................................. 34 Table 2.3: Exclusive breastfeeding recommendations from guidelines in high-income countries, according to infant age .................................................................................................................................................... 37 Table 2.4: Optimal breastfeeding recommendations from guidelines in high-income countries, according to infant age .................................................................................................................................................... 39 Table 2.5: Complementary feeding recommendations from guidelines in high-income countries ..................... 40 Table 2.6: Salt and sugar recommendations from guidelines in high-income countries .................................... 45 Table 2.7: Beverage recommendations from guidelines in high-income countries ........................................... 47 Table 2.8: Methods of dietary assessment .................................................................................................... 50 Table 2.9: Tools available for the assessment of feeding behaviours in infants and children ............................. 64 Chapter 3 Table 3.1: Foods included and excluded in food group analyses ................................................................... 109 Table 3.2: Consumption of breast milk and formula at least once and during both diet recalls days according to infant age .................................................................................................................................................. 110 Table 3.3: Consumption of vegetables at least once and during both diet recalls days according to infant age…………………………………………………………………………………………………………………………………………………………………..112 Table 3.4: Consumption of fruit at least once and during both diet recalls days according to infant age ......... 113 Table 3.5: Consumption of grain foods at least once and during both diet recalls days according to infant age. ............................................................................................................................................................... .113 Table 3.6: Consumption of milk and milk products at least once and during both diet recalls days according to infant age .................................................................................................................................................. 114 Table 3.7: Consumption of meat and protein-rich foods at least once and during both diet recalls days according to infant age .............................................................................................................................................. 115 X Table 3.8: Consumption of discretionary food at least once and during both diet recalls days according to infant age ........................................................................................................................................................... 116 Table 3.9: Consumption of commercial infant food at least once and during both diet recalls days according to infant age .................................................................................................................................................. 117 Chapter 4 Table 4.1: Indicators developed from the Ministry of Health Infant Feeding Guidelines ................................. 136 Table 4.2: Foods included and excluded in food group analyses ................................................................... 140 Table 4.3: Demographic characteristics of infants and caregivers (n=625) ..................................................... 142 Table 4.4: Summary of adherence to indicators developed from the Ministry of Health Infant Feeding Guidelines ................................................................................................................................................................ 143 Table 4.5: Sociodemographic characteristics associated with adherence to breastfeeding recommendations .............................................................................................................................................................. ..145 Table 4.6: Sociodemographic characteristics associated with adherence to solid food introduction recommendations ..................................................................................................................................... 147 Table 4.7: Sociodemographic characteristics associated with adherence to food variety recommendations on both 24-hour recall days ............................................................................................................................ 150 Table 4.8: Sociodemographic characteristics associated with adherence to the recommendation of not adding salt and sugar ............................................................................................................................................ 153 Table 4.9: Sociodemographic characteristics associated with adherence to spoon-feeding recommendations ............................................................................................................................................................... .154 Chapter 5 Table 5.1: Feeding behaviour criteria for PediEAT total and subscale scores ................................................. 174 Table 5.2: PediEAT norm reference scores for total PediEAT and subscales and participant mean score, with score ranges .............................................................................................................................................. 176 Table 5.3: Sociodemographic characteristics associated with infants in the ‘no concern’ and ‘concern’ categories for total PediEAT, physiologic symptoms, problematic mealtime behaviours, and selective/restrictive feeding behaviours (n=554) ................................................................................................................................... 177 Table 5.4: Food group consumption on both diet recall days associated with infants in the ‘no concern’ and ‘concern’ categories for total PediEAT, physiologic symptoms, problematic mealtime behaviours, and selective/restrictive eating (n=554) ............................................................................................................ 180 XI LIST OF FIGURES Chapter 3 Figure 3.1: First Foods New Zealand study screening and consent pathway .................................................. 106 Figure 3.2: Food group consumption at least once and during both diet recall days according to infant age ... 111 Chapter 4 Figure 4.1: (A) Exclusive breastfeeding prevalence (between birth and 4.9 months). (B) The percentage of infants receiving breast milk according to infant age. n=612. ...............................................................................144 Figure 4.2: Infant age when solid foods were introduced (n=625) ................................................................. 146 Chapter 5 Figure 5.1: First Foods New Zealand study flow diagram of recruitment, consent, and those included in the final analysis ..................................................................................................................................................... 175 XII LIST OF ABBREVIATIONS AND SYMBOLS Abbreviation or Symbol Definition AAP American Academy of Pediatrics AYCE About Your Child’s Eating BAMBI Brief Autism Mealtime Behaviour Inventory BLISS Baby-Led Introduction to Solids Study BLW Baby-led weaning BMI Body mass index BPFAS Behavioral Pediatrics Feeding Assessment Scale CDC Centers for Disease Control and Prevention CF Complementary feeding CFB Concerning feeding behaviours CEBI Children’s Eating Behaviour Inventory CEBQ Child Eating Behaviour Questionnaire CEDQ Children’s Eating Difficulties Questionnaire CIF Commercial infant food DONALD DOrtmund Nutritional and Anthropometric Longitudinally Designed Study DQIs Diet quality indices Dr Doctor EBF Exclusive breastfeeding e.g., For example ESPGHAN European Society for Paediatric Gastroenterology Hepatology and Nutrition Et al. and others FITS Feeding Infants and Toddlers Study FSANZ Food Standards Australia and New Zealand GUiNZ Growing up in New Zealand study HRC Health Research Council XIII Abbreviation or Symbol Definition ICFET International Complementary Feeding Evaluation Tool ICFI Infant and Young Child Feeding Indexes InFANT INfant Feeding, Active play and NuTrition Program MBQ Mealtime Behaviour Questionnaire MCHS Montreal Children’s Hospital Feeding Scale Meat and protein-rich foods Legumes, nut butters, eggs, fish, seafood and chicken, or lean red meat MoH Ministry of Health NZ New Zealand OzFITS The Australian Feeding Infants and Toddler Study PASSFP Paediatric Assessment Scale for Severe Feeding Problems PAHO Pan American Health Organization PubMed Medline Public Publisher RCT Randomised control trial SACN Scientific Advisory Committee on Nutrition SD Standard deviation STEP-CHILD Screening Tool of Feeding Problems Applied to Children TSF Traditional spoon-feeding UNICEF United Nations Children's Fund UK United Kingdom USA United States of America WHO World Health Organization YFNZ Young foods New Zealand XIV LIST OF PAPERS AND CONFERENCE PRESENTATIONS Three papers, shown below, were written during the PhD candidature to meet the requirements of thesis by publication. Manuscripts are formatted for publication and will be submitted in the upcoming months. DRC 16 statements are presented in Appendix 2. Methods for the First Foods New Zealand Study have been published (Appendix 3), with the candidates input. • Paper I: Contributions of Key Foods and Food Groups to the Dietary Intake of Infants in the First Foods New Zealand Study Paper II: Submitted to the Nutrients Journal 'Selected Papers from the 56th Annual Nutrition Society of New Zealand Conference' Adherence to Infant Feeding Guidelines in the First Foods New Zealand Study • Paper III: Parent-reported Feeding Behaviours of New Zealand Infants and Associations with Demographic Characteristics and Feeding Practices in the First Foods New Zealand Study • Methods (Appendix 3) Taylor RW, Conlon CA, Beck KL, von Hurst PR, Te Morenga LA, Daniels L, Haszard JJ, Meldrum AM, McLean NH, Cox AM, Tukuafu L, Casale M, Brown KJ, Jones EA, Katiforis I, Rowan M, McArthur J, Fleming EA, Wheeler BJ, Houghton LA, Diana A, Heath AM. Nutritional implications of baby-led weaning and baby food pouches as novel methods of infant feeding: Protocol for an observational study. JMIR Research Protocols, 10(4), e29048. https://doi.org/10.2196/29048 Conference presentations 1. Nutrition Society conference: 1st and 2nd December 2022 ‘Adherence to Breastfeeding and Complementary Feeding Guidelines within the First Foods New Zealand Study’. This research was extracted from paper II: Adherence to Infant Feeding Guidelines in the First Foods New Zealand Study. Awarded runner-up for best oral presentation. 1 CHAPTER 1 AN INTRODUCTION TO THIS RESEARCH Starting with an introduction to infant feeding, this chapter provides a background and justification for the thesis. The introductory section is then followed by the thesis’s aims, objectives, hypotheses, thesis structure, and author contributions. 2 1.1. INTRODUCTION AND JUSTIFICATION FOR THE STUDY The provision of safe and nutritionally appropriate foods in early life sets a foundation for good health across an individual’s lifespan (Jang & Serra, 2014). However, unlike other life stages, food choices rapidly change during infancy, as infants transition from breast or formula milk as a sole food source to a diet consisting of a range of foods and beverages from the family diet (Birch et al., 2007; Grummer- Strawn et al., 2008; Lioret et al., 2013). This can be a vulnerable phase of nutrition as an infant learns about new foods, tastes, and textures, developing food preferences that can last a lifetime (Berti & Agostoni, 2017; Birch & Doub, 2014; Nicklaus & Remy, 2013; Robinson & Fall, 2012). If poor food preferences and feeding behaviours are developed, individuals are more likely to make undesirable food choices in later life, increasing their risk of poor health. The diet during infancy, therefore, presents a window of opportunity to protect and promote good nutrition and healthy eating behaviours to support an individual’s immediate and long-term health. What is optimal infant nutrition? Until ‘around’ six months of age, an infant should receive all nutrition from breast milk (Ministry of Health, 2021a). Exclusive breastfeeding, or the provision of only breast milk for the first six months of life, has beneficial outcomes for both mothers and their offspring (Ministry of Health, 2021a). Infants who are breastfed have increased immune development and tolerance (Field, 2005), cognitive function (Anderson et al., 1999), and maternal bond (Horta & Victora, 2013). They also have a reduced risk of allergies (Robinson & Fall, 2012), gastrointestinal and respiratory infections (Duijts et al., 2009; Sankar et al., 2015), type two diabetes mellitus (Horta et al., 2015; Horta & Victora, 2013; Koletzko et al., 2019), and becoming overweight or obese (Arenz et al., 2004; Bartok & Ventura, 2009). Infants may also have an increased acceptance of new foods because of exposure to maternal dietary flavours in breast milk (Spahn et al., 2019; Stoody et al., 2019). Around the age of six months, breast milk is unable to provide adequate energy and nutrients, and solid foods should be introduced in addition to breast milk (Fewtrell et al., 2017). Breast milk, however, continues to be the greatest source of energy and total nutrients until one year of age (Ministry of Health, 2021a). The early introduction of solid foods, defined as before four months of age (Ministry of Health, 2021a), is associated with adverse health outcomes, including an increased risk of allergies (Fiocchi et al., 2006; Robinson & Fall, 2012), obesity (The Scientific Advisory Committee on Nutrition, 2018; Weng et al., 2012), and gastrointestinal damage (Butte et al., 2004; Fewtrell et al., 2017). An infant’s renal system 3 is also not physiologically mature enough to handle solid foods (Butte et al., 2004). Around six months of age, an infant is developmentally ready to start complementary feeding (CF) and it is recommended that spoon-fed puréed foods are introduced (Fewtrell et al., 2017). The Ministry of Health (MoH) does not recommend using baby-led weaning (BLW), where an infant self-feeds finger foods from the start of CF, as further research is required to determine if it is a nutritionally safe and developmentally appropriate feeding method (Ministry of Health, 2021a). Instead, infants should be encouraged to participate in mealtimes and self-feed when developmentally appropriate (Ministry of Health, 2021a). This allows infants to learn how to eat, enjoy mealtimes, follow their natural hunger and fullness cues, and learn how new foods taste and smell. A variety of foods from each of the food groups should be introduced into the diet after CF has started, including vegetables; fruit; grain foods; milk and milk products; and legumes, nut butters, eggs, fish, seafood and chicken or lean red meat (referred to as meat and protein-rich foods in this thesis), progressing from a puréed texture to lumpy, mashed, and soft finger foods (Ministry of Health, 2021a). Each food group provides important nutrients for infant growth and development. However, not all foods are equal, and a variety of foods within each food group are required to meet daily nutrient requirements. Iron-rich foods are particularly important due to declining iron stores, and foods such as red meat, fish, poultry, and iron-fortified infant cereals, should be offered daily to meet infants' high iron requirements (EFSA Panel on Nutrition et al., 2019). Foods with different flavours, including naturally sweet, savoury, and bitter, should also be introduced to widen an infant’s taste preferences (Ministry of Health, 2021a) and reduce fussy eating tendencies (de Barse et al., 2017). Commercial infant foods (CIF) can be included in an infant’s diet if used in addition to home-cooked foods (Ministry of Health, 2021a). Pouches and mixed commercial products should be emptied into a bowl to allow infants to see, smell, and touch the food. CIFs are a convenient source of infant nutrition, however, if overused and not used in addition to home-cooked meals, they may reduce the variety of flavours and textures in an infant's diet (World Health Organization, 2021). For pouches, incorrect feeding through the nozzle instead of a spoon may also increase the risk of concerning feeding behaviours (CFB), overfeeding, and poor dental health (Theurich, 2018). Caregivers are also recommended to avoid feeding infants foods and beverages containing or with added salt and sugar. Adding salt or sugar changes the flavour of food and accustoms taste preferences for sweet and salty, which, when consumed in large amounts, are linked to heart disease and diabetes (Michaelsen, 2000; World Health Organization, 2015). Beverages such as juice, cordial, fruit drinks, flavoured milk, soft drinks, tea, and coffee should also be avoided as they do not enhance an infant’s nutrition and may promote undesired taste preferences (Ministry of Health, 2021a). Cow’s milk does not contain adequate nutrients 4 compared to breast and formula milk and should also be avoided as a beverage until one year of age (Ministry of Health, 2021a; World Health Organization, 2009). ‘Normal’ feeding behaviours are not well defined and many infants experience difficulties when learning to eat (Pados et al., 2018). Responses such as gagging and refusal of new or unfamiliar foods are normal and typically resolve during the CF period. Behaviours are characterised as of concern when ongoing or when an infant will not eat enough despite the availability of appropriate foods (Kedesdy & Budd, 1998). Infant feeding guidelines in New Zealand The importance of infant feeding is recognised worldwide, as shown by the development of international and country-specific infant feeding recommendations. In New Zealand (NZ), dietary recommendations for infants and toddlers are guided by the recently updated ‘Healthy Eating Guidelines for New Zealand Babies and Toddlers (0-2 years)’ (Ministry of Health, 2021a). The 2021 released guidelines include six evidence-based eating statements to support optimal feeding practices for breastfeeding, CF, diet variety, appropriate foods, appropriate beverages, and eating environments. What do we know about infant nutrition in New Zealand? Many countries have reasonably up-to-date evidence on infant food or nutrient intake (Eldridge et al., 2019; Fox et al., 2004; Friel et al., 2010; Grummer-Strawn et al., 2008; Lennox et al., 2013; Lioret et al., 2013; United Nations International Children's Emergency Fund, 2021; White et al., 2017). New Zealand, however, has little comparable evidence (Taylor et al., 2021), with no national health or nutrition surveys undertaken in infants. Instead, most evidence regarding infant nutrition comes from the Growing Up in NZ (GUiNZ) study. GUiNZ is a longitudinal nationally generalizable study of 6470 mother and infant dyads born between 2007 and 2010 (Morton et al., 2012). A wealth of information was collected on infant feeding practices and dietary intake, including the incidence of breastfeeding, adherence to the 2008 MoH infant feeding guidelines, sociodemographic characteristics associated with guideline adherence, and food consumption at nine months of age (Castro et al., 2021; Ferreira et al., 2023; Gontijo de Castro et al., 2018; Morton et al., 2012; Morton et al., 2014). In recent years, other NZ studies have focused on specific areas of infant nutrition, including the nutritional outcomes when following BLW (Cameron et al., 2012; Cameron et al., 2013; Fu et al., 2018; Morison et al., 2016; Taylor et al., 2021) and adherence to specific dietary guidelines (Ministry of Health, 2021b). These studies 5 provide some insight into the current nutrition and feeding practices of infants. However, many, excluding the national health survey, were unable to represent the ethnic diversity of the NZ population, reducing generalisability, with notable associations between infant feeding practices and sociodemographic characteristics noted in GUiNZ (Morton et al., 2012). Various methods are available to assess feeding behaviours, including questionnaires and coding tools. Parent-reported questionnaires are minimally burdensome, affordable, highly repeatable, suitable for tracking feeding behaviours over time, and appropriate for research (Thoyre et al., 2014). The Paediatric Eating Assessment Tool (PediEAT) is a validated parent-reported questionnaire that measures CFB. Unlike most other questionnaires, PediEAT uses subscales to describe the type of CFB, including physiologic symptoms, problematic mealtime behaviours, selective/restrictive eating, and oral processing concerns (Thoyre et al., 2014). Parent concerns about infant feeding behaviours are commonly reported in high-income countries (Benjasuwantep et al., 2013; Goday et al., 2019). The prevalence of CFB in NZ infants is currently unknown, although other high-income countries have reported that at least 25% of typically developing children (Benjasuwantep et al., 2013; Goday et al., 2019) and 80% of children with developmental disabilities or diagnosed medical conditions (Gal et al., 2011; Goday et al., 2019) have CFB. Behaviours have also been associated with sociodemographic characteristics, with a higher prevalence of CFB in older and male children (Antoniou et al., 2016; Brown et al., 2018; Chilman et al., 2021; Tharner et al., 2014; Wardle et al., 2001), those living in high deprivation, and caregivers with low household income or education level (Emmett et al., 2018; Qiu & Hou, 2020; Tharner et al., 2015). Typically children with CFB are less likely to consume fruits and vegetables (Carruth et al., 1998; Perry et al., 2015; Switkowski et al., 2020). Previously breastfeeding has been associated with improved food acceptance in infants (Hausner et al., 2009; Mennella et al., 2017; Mennella et al., 2001; Sullivan & Birch, 1994) and reduced incidence of fussy eating tendencies behaviours in children (Cooke et al., 2004; Galloway et al., 2003). This is thought to be the result of exposure to a variety of flavours from the maternal diet. During the last decade, there have been significant changes in how infants are fed, with increasing availability of CIF (Katiforis et al., 2021; Padarath et al., 2020) and trends surrounding BLW (Brown et al., 2017; Cameron et al., 2013). In 2020, 266 CIF foods from 19 brands were found in two major NZ supermarket chains (Foodstuffs and Woolworths) (Katiforis et al., 2021), and a recent report showed a 27% increase in products available in the baby food sector between 2010 and 2016 in NZ (GlobalData, 2016). The use of CIF has likely changed since GUiNZ. However, despite the increasing availability of 6 CIF, there is limited evidence available to determine the use of CIF, feeding methods used (e.g., spoon- feeding or direct consumption from products), and the influence products have on nutrient intake in NZ (Katiforis et al., 2021; Rowan et al., 2022). However, BLW seems to be growing in popularity (Brown et al., 2017; Cameron et al., 2013) despite not being recommended by the MoH (Ministry of Health, 2021a). Our knowledge surrounding infant feeding in NZ is therefore limited, and with the emergence of BLW (Brown et al., 2017; Cameron et al., 2013) and the increasing availability of CIF (Katiforis et al., 2021; Padarath et al., 2020), there are likely to be significant changes in what and how infants are fed. Introducing the First Foods NZ study First Foods New Zealand (FFNZ) is a Health Research Council (HRC) funded observational study of 625 Dunedin and Auckland infants aged 7.0 to 10.0 months. FFNZ aims to provide up-to-date insight into the changes that occur as an infant progresses from a diet consisting of 100% milk to one that resembles family meals. This information will allow a greater understanding of what NZ whānau (families) are feeding their infants and their feeding methods. Key outcomes of FFNZ include infant iron status, growth, food and nutrient intake, breastfeeding incidence, eating and feeding behaviours, dental health, oral motor skills, and choking risk of New Zealand infants in general and those using pouches or BLW. This thesis will provide information on infant food and food group consumption, adherence to key MoH infant feeding guidelines, and parent-reported feeding behaviours in NZ infants between 7.0 and 10.0 months of age. This information will provide the MoH, health professionals, and NZ health and wellbeing organisations such as Plunket with up-to-date evidence on infant nutrition in NZ. 7 1.2. THESIS AIMS The overall aims of this thesis were to investigate the following in NZ infants between 7.0 and 10.0 months of age: 1) Contributions that key foods and food groups make to the dietary intake of NZ infants, 2) Adherence of infants to key dietary indicators based on the Ministry of Health’s Healthy Eating Guidelines for New Zealand Babies and Toddlers (0–2 years old), 3) Describe parent-reported feeding behaviours of NZ infants and associations with demographic characteristics and feeding practices. 1.3. THESIS OBJECTIVES 1. Explore the contributions that key foods and food groups make to the dietary intake of NZ infants between 7.0 and 10.0 months of age. 1.1. Describe the food and food groups contributing to the dietary intake of NZ infants, 1.2. Describe differences in food and food group intake in NZ infants between 7.0 to 10.0 months of age, 1.3. Describe the contribution of commercial infant foods to the dietary intake of NZ infants. 2. Investigate adherence to key dietary indicators of NZ infants between 7.0 and 10.0 months of age, as guided by the Ministry of Health’s Healthy Eating Guidelines for New Zealand Babies and Toddlers (0–2 years old). 2.1. Describe adherence to exclusive and current breastfeeding guidelines, 2.2. Describe adherence to complementary feeding guidelines, including the age of solid introduction, appropriate foods introduced, appropriate textures, and appropriate feeding styles, 2.3. Describe adherence to food variety guidelines, including the consumption of vegetables, fruit, grain foods, milk and milk products, and meat and protein-rich foods (legumes, nut butters, eggs, fish, seafood, chicken, or lean red meat), 2.4. Describe adherence to appropriate food and beverage guidelines, including the offering of appropriate drinks and avoiding the addition of salt and sugar, 2.5. Describe adherence to self-feeding guidelines, 2.6. Determine sociodemographic characteristics associated with guideline adherence. 8 3. Describe parent-reported feeding behaviours of NZ infants between 7.0 and 10.0 months of age and associations with demographic characteristics and feeding practices. 3.1. Describe parent-reported feeding behaviours of NZ infants, according to the Paediatric Eating Assessment Tool (PediEAT) total and subscale scores, 3.2. Describe associations between parent-reported feeding behaviours and infant and parent demographic characteristics, 3.3. Describe associations between parent-reported feeding behaviours and infant feeding practices (current breastfeeding and food group consumption). 1.4. STUDY HYPOTHESES 1. Key food contributors within the five food groups will differ between infants of different ages. Older infants will be more likely to meet the MoH food group recommendations. 2. Adherence to key indicators will vary between infants with different demographic characteristics, including maternal age, education, parity and employment status, early child education centre attendance, the number of children in the household, and deprivation. 3. Differences in parent-reported feeding behaviours according to infant and parent demographics and feeding practices will be observed. Infants with concerning parent-reported feeding behaviours will be less likely than infants without to consume the MoH food groups (vegetables, fruit, grain foods, milk and milk products, and meat and protein-rich foods) and more likely to consume CIF. 1.5. THESIS STRUCTURE This thesis is divided into six chapters. Chapter one is an introductory chapter, providing a background and justification of the study, aims, objectives, hypotheses, and author contributions. A narrative review in chapter two outlines the current NZ and international recommendations for feeding infants and critically reviews the current evidence on infant nutrition and dietary intake, adherence to key dietary indicators, and dietary behaviours. Chapters three, four, and five present research findings, answering the study's three aims. These chapters are presented as manuscripts for publication with the inclusion of abstracts, introductions, methods, results, discussion, references and links to appendices 9 as relevant to the chapter. The final chapter, chapter six, provides a discussion of the key study findings, research outcomes, study limitations, and directions for future research. The appendices include relevant documents to support an understanding of the study, including recruitment resources, the published FFNZ protocol paper, participant information sheets, relevant standard operating procedures (SOPs), resources used during data collection, copies of the main and final questionnaires, and the candidates’ abstract that was published in the Nutrition Society of NZ conference proceedings. 1.6. RESEARCHER CONTRIBUTIONS This thesis uses data from the First Foods NZ study (FFNZ). The candidate played a significant role in all aspects of the study, including writing and reviewing study protocols, recruitment, data collection (both Auckland and Dunedin), data quality checking, data management and cleaning, development of the FFNZ food coding system, food and food group coding, and data analysis. The candidate was involved in all aspects of FFNZ data collection, including the collection of infant anthropometry, infant dental images, and saliva samples from breastfeeding mothers and infants. This thesis does not discuss these outcomes; they are part of the wider FFNZ study aims. The candidate also collected data for an associated study: Young Foods New Zealand (YFNZ), as cohorts overlapped when multiple children resided in a home. An overview of contributions made by the candidate are available in Table 1.1. Several individuals were involved in FFNZ and contributed to this research, as discussed below: Professor Anne-Louise Heath and Professor Rachael Taylor were the study’s primary investigators and led the research. They oversaw the overall FFNZ study, designed and developed the study, applied for research funding and ethics approval, developed the study protocols, assisted in protocol training, and mentored and allocated student roles. Professor Cathryn Conlon, Professor Pamela von Hurst, Associate Professor Kathryn Beck, Associate Professor Lisa Te Morenga, and Associate Professor Jill Haszard were FFNZ co-investigators. They were involved in the design and development of the study, research funding applications, and obtaining ethical approval. Additionally, they reviewed study protocols, questionnaires and data collection strategies. Professor Cathryn Conlon (main), Professor Pamela von Hurst (co), and Associate Professor Kathryn Beck (co) were the candidate’s supervisors. They mentored the candidate, provided academic support for the development and design of this thesis, reviewed and revised each thesis chapter, assisted in the dissemination of the results, and approved the thesis for submission. 10 Lisa Daniels (postdoctoral fellow) was involved in the study conceptualisation, writing, and review of protocols. Associate Professor Lisa Te Morenga provided cultural advice and ensured that FFNZ researchers and publications were culturally safe and met the needs of the NZ population. Associate Professor Jillian Haszard provided statistical support for data analysis and the dissemination of the study results. She was involved in the development of student aims and objectives, statistical method design, data cleaning, statistical analysis, and dissemination of results. Jenny McArthur and Rebecca Paul assisted in recruitment, participant bookings, and data collection in Dunedin and Auckland, respectively. They worked closely with FFNZ and YFNZ PhD students Maria Casale, Emily Jones, Rio Jupiterwala, Neve McLean, Alice Cox, Ioanna Katiforis, Lesieli Tukuafu, and Master’s students Madeline Rowan, Madeline Gash, Bailey Bruckner, and Annabelle Malone, who assisted in the development and reviewing of study protocols, protocol training, recruitment, data collection, data entry, data cleaning, and dissemination of results in their specific areas of research. Additional support for data collection was received from research assistants Andrea Wei, Emily Shoesmith, Darrian Holten, Ella Brouwer, Marsha Piddington, and Shay Whickham. Elizabeth Fleming, Elizabeth Jones, and Ioanna Katiforis led FoodWorks data entry, with the candidate conducting quality checking of recalls. The candidate and Ioanna Katiforis then developed the FFNZ food coding system under the supervision of Elizabeth Fleming and Professor Anne-Louise Heath. Ioanna Katiforis and the candidate were responsible for coding foods consumed during the diet recalls. 11 Table 1.1: Candidate research contribution Stage Task Contribution Study development Study protocols Assisted in the development of the recruitment, food recall, photo transfer, final questionnaire, and frequently asked question protocols. Reviewed all study protocols and advised revisions. Study questionnaires Assisted in the development of the main questionnaire. Reviewed the main and final questionnaires. Participant forms Assisted in the development of the participant information sheet, consent form, and diet recall forms. Training Engaged in student training for all areas of FFNZ data collection, including peer observations of 24-hour diet recalls. Adherence indicator Developed the indicators presented in chapter three (Adherence to infant feeding guidelines) based on the 2021 Ministry of Health infant feeding guidelines and available data from the study to assess adherence to the infant feeding guidelines. Food coding system Developed FFNZ’s food group and ingredient coding system and trained other team members to use the standard operating procedures and codebooks. Flow chart Developed the flow charts for Auckland and Dunedin to support researchers. Charts included a study timeline, equipment required at each visit, and visit objectives. Charts were used by researchers to prepare for appointments and ensure appropriate equipment was available during appointments. Recruitment Assisted in the development and design of advertisements, posters, media content, and the FFNZ website and Facebook account. Assisted in the promotion of FFNZ and developed targeted recruitment strategies for Māori and Pacific participants. Assisted in screening new participants and scheduling appointments. Data collection Participant visits Collected data in both Auckland and Dunedin. Conducted onsite, home, and online appointments in both locations. Tracking documents Designed and monitored documents for tracking new and current participants for Auckland and Otago. This ensured that all researchers understood what the participant had completed and were yet to complete. Follow-up contacts and notes were added when required (e.g. requested diet recall images on 21 April). Ethnicity targets Designed Red-Cap reports to track ethnicity numbers. This aided the wider team to monitor study targets during data collection. Student + research assistant support Supported new students and research assistants during study visits and administration. 12 Stage Task Contribution Data management FoodWorks quality check Assisted in FoodWorks data quality checking – checking 100% of formula entries and 45% of total recalls. Engaged in weekly team meetings to discuss data errors for FFNZ and YFNZ. Food groupings Individually coded foods (n=12,628) into food groups and ingredients after discussions with the supervisory team. Supported the YFNZ team to complete ingredient and food group coding (n=19,577) Engaged in weekly meetings to discuss problematic foods and coding requirements. Data analysis Worked alongside the study statistician to review data quality and results presented in this thesis. All data analysis strategies were initially developed by the candidate and confirmed after consultation with the study statistician. Analysed all food and food group data presented in manuscript one and the food group, salt, and sugar data presented in manuscript two. 13 1.7. REFERENCES Anderson, J. W., Johnstone, B. M., & Remley, D. T. (1999). Breast-feeding and cognitive development: A meta-analysis. 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E., Daniels, L. A., & Magarey, A. M. (2015). Food neophobia and its association with diet quality and weight in children aged 24 months: A cross sectional study. International Journal of Behavioral Nutrition and Physical Activity, 12(1), 1-8. https://doi.org/10.1186/s12966-015-0184-6 Qiu, C., & Hou, M. (2020). Association between food preferences, eating behaviors and socio- demographic factors, physical activity among children and adolescents: A cross-sectional study. Nutrients, 12(3), 640. https://doi.org/10.3390/nu12030640 Robinson, S., & Fall, C. (2012). Infant nutrition and later health: A review of current evidence. Nutrients, 4(8), 859-874. https://doi.org/10.3390/nu4080859 19 Rowan, M., Mirosa, M., Heath, A.-L. M., Katiforis, I., Taylor, R. W., & Skeaff, S. A. (2022). A qualitative study of parental perceptions of baby food pouches: A netnographic analysis. Nutrients, 14(15), 3248. https://doi.org/10.3390/nu14153248 Sankar, M. J., Sinha, B., Chowdhury, R., Bhandari, N., Taneja, S., Martines, J., & Bahl, R. (2015). Optimal breastfeeding practices and infant and child mortality: A systematic review and meta-analysis. Acta Paediatrica, 104, 3-13. https://doi.org/10.1111/apa.13147 Spahn, J. M., Callahan, E. H., Spill, M. K., Wong, Y. P., Benjamin-Neelon, S. E., Birch, L., Black, M. M., Cook, J. T., Faith, M. S., & Mennella, J. A. (2019). Influence of maternal diet on flavor transfer to amniotic fluid and breast milk and children's responses: A systematic review. The American Journal of Clinical Nutrition, 109(Supplement 1), 1003S-1026S. https://doi.org/10.1093/ajcn/nqy240 Stoody, E. E., Spahn, J. M., & Casavale, K. O. (2019). The pregnancy and birth to 24 months project: A series of systematic reviews on diet and health. The American Journal of Clinical Nutrition, 109(Supplement 1), 685S-697S. https://doi.org/10.1093/ajcn/nqy375 Sullivan, S. A., & Birch, L. L. (1994). Infant dietary experience and acceptance of solid foods. Pediatrics, 93(2), 271-277. https://pubmed.ncbi.nlm.nih.gov/8121740/#:~:text=Conclusions%3A%20Infants%20increase %20their%20acceptance,the%20acceptance%20of%20solid%20foods. Switkowski, K. M., Gingras, V., Rifas-Shiman, S. L., & Oken, E. (2020). Patterns of complementary feeding behaviors predict diet quality in early childhood. Nutrients, 12(3), 810. https://doi.org/10.3390/nu12030810 Taylor, R. W., Conlon, C. A., Beck, K. L., von Hurst, P. R., Te Morenga, L. A., Daniels, L., Haszard, J. J., Meldrum, A. M., McLean, N. H., & Cox, A. M. (2021). Nutritional implications of baby-led weaning and baby food pouches as novel methods of infant feeding: Protocol for an observational study. JMIR Research Protocols, 10(4), e29048. https://doi.org/10.2196/29048 Tharner, A., Jansen, P. W., Kiefte-de Jong, J. C., Moll, H. A., Hofman, A., Jaddoe, V. W., Tiemeier, H., & Franco, O. H. (2015). Bidirectional associations between fussy eating and functional constipation in preschool children. The Journal of Pediatrics, 166(1), 91-96. e91. https://doi.org/10.1016/j.jpeds.2014.09.028 Tharner, A., Jansen, P. W., Kiefte-de Jong, J. C., Moll, H. A., van der Ende, J., Jaddoe, V. W., Hofman, A., Tiemeier, H., & Franco, O. H. (2014). Toward an operative diagnosis of fussy/picky eating: A latent profile approach in a population-based cohort. International Journal of Behavioral Nutrition and Physical Activity, 11, 1-11. https://doi.org/10.1186/1479-5868-11-14 20 The Scientific Advisory Committee on Nutrition. (2018). Feeding in the first year of life. The Scientific Advisory Committee on Nutrition. https://www.gov.uk/government/publications/feeding-in- the-first-year-of-life- sacnreport#:~:text=SACN%20recommends%20retaining%20existing%20advice,around%206% 20months%20of%20age. Theurich, M. A. (2018). Perspective: Novel commercial packaging and devices for complementary feeding. Advances in Nutrition, 9(5), 581-589. https://doi.org/10.1093/ADVANCES/NMY034 Thoyre, S. M., Pados, B. F., Park, J., Estrem, H., Hodges, E. A., McComish, C., Van Riper, M., & Murdoch, K. (2014). Development and content validation of the pediatric eating assessment tool (Pedi-EAT). American Journal of Speech-Language Pathology. https://doi.org/10.1044/1058-0360(2013/12-0069) United Nations International Children's Emergency Fund. (2021). Too many children are not eating the nutrient-rich foods they need to grow and develop. https://data.unicef.org/topic/nutrition/diets/ Wardle, J., Guthrie, C. A., Sanderson, S., & Rapoport, L. (2001). Development of the children's eating behaviour questionnaire. Journal of Child Psychology and Psychiatry, 42(7), 963-970. https://doi.org/10.1111/1469-7610.00792 Weng, S. F., Redsell, S. A., Swift, J. A., Yang, M., & Glazebrook, C. P. (2012). Systematic review and meta-analyses of risk factors for childhood overweight identifiable during infancy. Archives of Disease in Childhood, 97(12), 1019-1026. https://doi.org/10.1136/archdischild-2012-302263 White, J. M., Bégin, F., Kumapley, R., Murray, C., & Krasevec, J. (2017). Complementary feeding practices: Current global and regional estimates. Maternal and Child Nutrition, 13, e12505. https://doi.org/10.1111/mcn.12505 World Health Organization. (2009). Infant and young child feeding: Model chapter for textbooks for medical students and allied health professionals. https://apps.who.int/iris/handle/10665/44117 World Health Organization. (2015). Infant feeding recommendation. https://www.who.int/news- room/fact-sheets/detail/infant-and-young-child feeding#:~:text=WHO%20and%20UNICEF%20recommend%3A,years%20of%20age%20or%20 beyond. World Health Organization. (2017). Exclusive breastfeeding for optimal growth, development and health of infants. Retrieved 5 May, 2022 from https://www.who.int/news/item/15-01-2011- exclusive-breastfeeding-for-six-months-best-for-babies- 21 everywhere#:~:text=WHO%20recommends%20mothers%20worldwide%20to,of%20two%20y ears%20or%20beyond. World Health Organization. (2021). Infant and young child feeding. Retrieved 5 May, 2022 from https://www.who.int/news-room/fact-sheets/detail/infant-and-young-child-feeding 22 CHAPTER 2 A REVIEW OF THE CURRENT LITERATURE THIS CHAPTER REVIEWS THE CURRENT LITERATURE ON INFANT DIETARY INTAKE, ADHERENCE TO INFANT FEEDING GUIDELINES, AND FEEDING BEHAVIOURS IN NEW ZEALAND AND HIGH-INCOME COUNTRIES. MULTIPLE SEARCH TERMS DERIVED FROM THE STUDY OBJECTIVES WERE USED BETWEEN JANUARY 2020 AND APRIL 2023. APPENDIX 1 PROVIDES FURTHER INFORMATION ON THE SEARCH STRATEGY. 23 2.1. INFANT FEEDING GUIDELINES Nutrition plays a crucial role in the first years of life, directly influencing infant growth (Dewey, 2001), development (Dewey, 2003; Ministry of Health, 2021a), and lifelong dietary habits (Matthews et al., 2019; Reidy et al., 2017; Schwartz et al., 2011; Young & Krebs, 2013). Inadequate nutrition during early life leads to stunting, poor cognitive development, and increased morbidity (Black et al., 2008), with the highest prevalence of undernutrition worldwide occurring during the complementary feeding (CF) period (Victora et al., 2010). Conversely, overfeeding has been associated with excessive weight gain and metabolic programming, influencing the development of non-communicable diseases (Hanson & Gluckman, 2011; Singhal, 2016; Wu & Chen, 2009). Due to the negative implications of under and overfeeding, the importance of optimal dietary choices during infancy is well known (World Health Organization, 2015b). To support optimal practices, infant feeding guidelines have been developed worldwide. 2.1.1. A GLIMPSE INTO THE HISTORY OF INFANT NUTRITION Feeding practices have evolved significantly in the past 250 years as knowledge surrounding infant feeding has increased (Jones, 2016). From early times, the importance of breastfeeding was recognised for infant growth and development. However, not all women were able or wanted to breastfeed (Radbill, 1981; Stevens et al., 2009; Wickes, 1953a). This led to many women using a wet nurse, ‘a woman who breastfeeds another's child’ before the introduction of early infant formulas (Stevens et al., 2009). Wet nursing provided an alternative means of feeding and became popular in the early 18th century for women of higher social class (Wickes, 1953b). However, when evidence emerged for the maternal benefits of breastfeeding, a shift was observed, with working-class women becoming the leading employers of wet nurses. This was common until 1900 when milk alternatives (animal milks) became popular (Minchin, 2018; Osborn, 1979). Early feeding practices were associated with high morbidity and mortality rates because of poor hygiene and sterilisation practices, the offering of low-nutrient or inappropriate foods, and sudden weaning from breast milk (Davies & O’Hare, 2004; Katzenberg et al., 1996; Weinberg, 1993). Recommendations for introducing solid foods were more ambiguous during early history. Like today, different recommendations were found in different parts of the world (Minchin, 2018). Until the Renaissance period, offering solid foods (such as meat, fruit, and vegetables) typically occurred at two 24 or three years of age, after the final milk tooth had erupted (Forsyth, 1911; Levin, 1959). However, in parts of the world, thin gruels, such as pap (bread soaked in water, milk, wine or beer) and panada (cereals cooked in meat or vegetable broth), were cautiously added around six months of age (Forsyth, 1911; Levin, 1959; Obladen, 2014). By the 15th and 16th centuries, reports appear that gruels were frequently fed from birth, often in the replacement of breast milk (Forsyth, 1911; Levin, 1959; Obladen, 2014). When gruels were of a thicker consistency, pre-mastication, where a parent or caregiver chewed food into a suitable texture before feeding to an infant, was common practice (Pelto et al., 2010). Until the end of the nineteenth century, animal milk was the most common source of artificial feeding (Radbill, 1981). Pap and panada were also commonly added to bottles/devices to support infants with faltering growth (Forsyth, 1911; Radbill, 1981; Wickes, 1953b). However, poor sanitisation practices resulted in a high mortality rate. Infant formulas started to appear in the 18th century, with the first powdered form produced and marketed in 1865 (Radbill, 1981). By 1883, 27 branded powdered infant food products were available in the United States of America (USA) (Fomon, 2001). Initially, these were poor sources of nutrition, however, over time, protein, vitamins, and minerals were added alongside recommendations for appropriate sanitation techniques (Fomon, 2001). Between 1940 and 1970, a steady decline in breastfeeding was observed, with many physicians and mothers promoting formula as a safe substitute for breastfeeding (Minchin, 2018). Because of this decline, regulations for the marketing of formula were put in place in the 1970s to support women who were not able to breastfeed (Fomon, 2001). Following the development of early infant formula, recommendations for introducing solid foods changed again, with solid food considered unsuitable before a year of age. However, many parents continued to offer gruels by six months of age. Commercial infant foods (CIF), including jars of apple purée, also appeared during this century and were highly recommended by paediatricians for those over one year of age (Radbill, 1981). In the late 19th and early 20th centuries, it was rare to offer solid foods before one year of age, following fears that this would harm ‘the delicate child’ (Fomon, 2001). However, the discovery of vitamins in 1912 (Funk, 1912) promoted change. By 1920, it was recommended that meat and liver were introduced during the first two weeks of life, followed by cereals, vegetables, and fruit (including juice), forming a mixed diet by one year of age (Jundell, 1923). The latter was a significant factor in minimising scurvy (Apple, 1987; McCollum, 1957). Rickets were also problematic at this time, and supplementation with cod-liver oil after birth became common practice in the USA (Apple, 1987; Holt, 1963). 25 By the 1950s, knowledge surrounding the physiological process of chewing and swallowing was developing, and guidelines started referencing the importance of an infant being able to transfer food from the front of the tongue to the pharynx for safe eating (Bakwin & Bakwin, 1953). Infants were subsequently offered an array of foods, including fruit (commonly banana), chocolate, juice, ice cream, custard, and vegetables from a young age (American Academy of Pediatrics Committee, 1958; Levin, 1959). In 1958 the American Academy of Pediatrics (AAP) released its first report on infant feeding, acknowledging that the developmental maturity of the gut, growth, and activity level of an infant were better indicators than age to determine if the introduction of solid foods was appropriate (American Academy of Pediatrics Committee, 1958). However, the report suggested that these indicators were typically present between three and four months of age. Since then, recommendations for the appropriate age to start solid foods have varied between eight weeks (1960s) (Harris & Chan, 1969; Jones et al., 1964), four months (1970s) (Challacombe, 1983; Fomon, 1975; Fomon & Anderson, 1972), and six to nine months (1990s) (Grimshaw et al., 2009); significantly influenced by a greater understanding of the development of allergies and coeliac disease (Koplin & Allen, 2013). Infant feeding in New Zealand New Zealand (NZ) shares a similar history, following a slightly later timeline (Apple, 1994). A key difference in NZ’s history was the inception of Plunket in 1907 by Sir Frederick Truby King (Bryder, 2008). Plunket, named after Lady Victoria Plunket, wife of the NZ governor at the time, aimed to promote maternal and infant health by providing care from trained nurses (Bryder, 2001). A decade after its initiation, NZ had the lowest infant mortality rate in the world (Bryder, 2017). A vital component of this success was the promotion of breastfeeding (Bryder, 2001), the opening of infant hospitals (Ryan, 1997), and recommendations of spoon-feeding solid foods rather than adding them to bottles (Jones, 2016; Ryan, 1997). Truby King recommended breastfeeding four-hourly between 6am-10pm, avoiding night feeding (Jones, 2016; Ryan, 1997). Additionally, infants were to receive fruit juice from two to three weeks of age and cod liver oil from one month. In the cases of failure to thrive, formula was supported. The introduction of solid foods was recommended to start no later than nine months of age, introducing food groups one at a time (Jones, 2016; Ryan, 1997). Over time, the appropriate age for CF changed with differing opinions of medical professionals and women (Minchin, 2018). Post-war, the cessation of breastfeeding was reported to occur earlier, with 40% of infants being weaned by 12 weeks of age (Bryder, 2005). Women returning to work was considered a factor in this shift, with married women accounting for 42% of working women in 1966 26 (Nolan, 2000). The prevalence of breastfeeding, however, improved as knowledge developed for the benefits of breastfeeding (Bryder, 2005). CF practices also improved as scientific evidence grew for feeding infants (Deem & Fitzgibbon, 1964). New feeding trends In recent years, how infants are fed has changed with the emergence of a new feeding method known as baby-led weaning (BLW). Gill Rapley first described BLW in 2005 (Rapley & Murkett, 2008) and has increased in popularity globally (Brown et al., 2017; Cameron et al., 2013) following the 2002 World Health Organization (WHO) guideline change, advising that CF should start at six months instead of the previously advised four months (World Health Organization, 2002). Given the significant developmental changes that occur between four and six months, advocates for BLW have suggested that an infant does not need purées or to be spoon-fed (Rapley & Murkett, 2008). Instead, they are physiologically able to self-feed family food. BLW is not currently recommended in NZ (Ministry of Health, 2021a). However, given its increase in popularity, several studies have investigated the potential benefits of better energy self-regulation and improved motor skills, and risks including choking, inadequate nutrient and energy intake, suboptimal iron status, and diet quality, with inconclusive results (Brown et al., 2017; Cameron et al., 2012; D’Auria et al., 2018; Fewtrell et al., 2017; Rapley, 2011; Rapley & Murkett, 2008; Townsend & Pitchford, 2012). It is not currently known how pervasive BLW is in NZ, although a recent NZ study (n=876) suggested that more than half of families have tried it, with approximately 30% following BLW regularly (Fu et al., 2018). This is higher than those observed in 2013, with 8% of caregivers (n=199) meeting strict BLW criteria (Cameron et al., 2013), however, lower than observations in the United Kingdom (UK) (30 to 60%) (Brown, 2016; Rowan et al., 2019), Ireland (49%) (Mezynska et al., 2020), and Australia (46%) (Swanepoel et al., 2020). Due to the nutritional risks of BLW, increased popularity, and widespread online presence (about 13,700,000 results; Google 28 April 2023), further research is required to determine the incidence and health risks of BLW so that health professionals and policymakers can provide whānau (families) with evidence-based advice on how to feed their infants safely. The availability of CIF has also changed in recent years, with a notable increase in products marketed towards infants in NZ (Katiforis et al., 2021; Padarath et al., 2020). In 2021, a study found 266 CIF available in two major NZ supermarket chains (Foodstuffs and Woolworths) (Katiforis et al., 2021). This was notably higher than those previously identified (Padarath et al., 2020). CIF are now available in various forms, including single-use food pouches (with and without plastic nozzles), jars, cans, 27 microwavable bowls, cereals, crackers, bars, biscuits, rusks, and extruded snacks. This is different to the glass jars of puréed fruit and vegetables seen in the past. Concern has been expressed about the nutritional content of CIFs, including the total energy, sugar, and iron contained in many products (Pask, 2020; Sundborn et al., 2017). The use of plastic nozzles to feed directly from pouches is also concerning, with the recent Australian Feeding Infants and Toddlers Study (OzFITS) (n=598) reporting that half of those who consumed a pouch (n=299) sucked the contents directly through the nozzle (Netting, Moumin, Makrides et al., 2022). In NZ, the utilisation of CIF and use of nozzles is unknown (Taylor et al., 2021). 2.1.2. THE NEW ZEALAND MINISTRY OF HEALTH INFANT FEEDING GUIDELINES The NZ ‘Food and Nutrition Guidelines for Healthy Infants and Toddlers (Aged 0–2)’ (Ministry of Health, 2008) were originally developed in 2008 and aimed to provide caregivers and health professionals with evidence-based recommendations specific to NZ. The series was recently transitioned and updated to include evidence-based recommendations for nutrition and physical activity, following an independent evaluation of the Ministry of Health’s (MoH) Food and Nutrition Guidelines in 2011 (Ministry of Health, 2020a). The 2021 infant guidelines, ‘Healthy Eating Guidelines for New Zealand Babies and Toddlers (0– 2 years old)’, were written by infant nutrition experts with the support of a maternal, infant, and toddler technical advisory group (