2026-09-26T13:59:45Zhttps://mro.massey.ac.nz/server/oai/requestoai:mro.massey.ac.nz:10179/175062025-11-11T14:14:47Zcom_10179_294col_10179_296
DSpace at Massey University
author
Fini, Tazyn
2022-08-23T21:16:04Z
2022-08-23T21:16:04Z
2022
http://hdl.handle.net/10179/17506
Background: Excess adipose tissue is associated with metabolic risk and developing obesity related diseases. A significant amount of people are unknowingly metabolically unhealthy, having a high body fat percentage (BF%) despite normal body mass index (BMI) classification. Evidence of microRNAs (miRNA; miR) as potential biomarkers of metabolic risk has emerged and may prove useful in identifying those at metabolic risk where BMI classification may fail them. Objectives: To explore miRNAs expression levels in body composition of healthy New Zealand (NZ) women and its association with metabolic markers, dietary and physical activity factors. Methods: Cross - sectional design investigating healthy NZ women (n = 406) of three ethnicities (Māori, Pacific, European) aged 16 to 45 years. Body mass index and BF% defined body profile groups; “NN” group - normal BMI (≥18.5 and <25kg/m2) and normal BF% (≥18%, <30%); “NH” group - normal BMI (<25 kg/m2) and high BF% (≥30%); “HH” group – high BMI (≥25 kg/m2) and high BF% (≥30%), of which 382, met the criteria. Anthropometry, metabolic biomarkers, miRNA, dietary, and physical activity factors were evaluated. Results: 105 (27.5%), 70 (18.3%), and 207 (54.2%) participants were classified as having NN, NH, and HH body profile, respectively. The adjusted (age, deprivation index and other miRNAs) odds of having higher miR-222-3p were increased in NH (OR = 1.92, 95% CI 1.13-3.26) and HH (OR = 2.58, 95% CI 1.58-4.21) versus NN group. The adjusted odds of having higher miR-29b-3p decreased in HH (OR = 0.09, 95% CI 0.02-0.37) versus NN group. Higher miR-222-3p (1.084-14.438 AU) was associated with HH body profile (p = 0.002), higher leptin levels (p = 0.04) and sucrose intake (p = 0.025) and lower protein intake (p = 0.017). Higher miR-29b-3p (0.202-1.851 AU) was associated with lower HbA1c (p = 0.016), TNF-α (p = 0.001), IL-6 (p = 0.016), IL-10 (p = 0.021) and higher sucrose intake (p = 0.049), and higher miR-17-5p (0.103-0.806 AU) was associated with higher TNF-α (p = 0.014) and lower IL-6 (p = 0.001). Conclusion: Our findings for miR-222-3p strongly support previous research, making it the most promising biomarker for obesity of the selected miRNA analysed in this study. Our study identified a selection of specific metabolic (Leptin, HbA1c) and inflammatory markers (IL6, IL-10, TNF-α) as well as dietary factors (sucrose, carbohydrate, protein) and light physical activity, to be associated with miR-222-3p, miR-29b-3p, and miR-17-5p. These findings suggest that miRNAs are involved in metabolic processes and, with further research, may be used as biomarkers of metabolic risk.
en
The Author
Exploring the relationships between microRNA expression, body composition and metabolic risk in healthy New Zealand women : a thesis completed as part of the requirements for Master of Science in Nutrition and Dietetics at Massey University, Albany Campus, Auckland, New Zealand
Thesis
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URL
https://mro.massey.ac.nz/bitstreams/6590d788-8c27-42bb-8d6c-e280f15295b7/download
File
MD5
74843997d23223f64754b3079e9cd026
1876772
application/pdf
FiniMScThesis.pdf
URL
https://mro.massey.ac.nz/bitstreams/173b7ed3-014a-47c7-8237-1b17ae37cca6/download
File
MD5
badf3953c2b8fbcd1c03758e9c437951
100350
text/plain
FiniMScThesis.pdf.txt