Barua MAbdollahi MRZaefarian FWester TJGirish CKChrystal PVRavindran V2023-12-122024-07-252021-05-212023-12-122024-07-252021-08Barua M, Abdollahi MR, Zaefarian F, Wester TJ, Girish CK, Chrystal PV, Ravindran V. (2021). Basal ileal endogenous amino acid flow in broiler chickens as influenced by age.. Poult Sci. 100. 8. (pp. 101269-).0032-5791https://mro.massey.ac.nz/handle/10179/70690The current study was carried out to measure the basal ileal endogenous amino acid (EAA) flow in male broilers (Ross 308) at different ages (d 7, 14, 21, 28, 35, and 42), following the feeding of a nitrogen-free diet. Titanium dioxide (5 g/kg) was included as an indigestible marker. The nitrogen-free diet was offered for four days prior to ileal digesta collection to 6 replicate cages housing 14 (d 3–7), 12 (d 10–14), 10 (d 17–21), 8 (d 24–28), 8 (d 31–35), and 6 (d 38–42) birds per cage. The basal EAA flow was calculated as g/kg DM intake. The amino acid (AA) profile of endogenous protein, expressed as g/100 g protein, was also calculated. The basal endogenous flow of nitrogen and all individual and total AA decreased quadratically (P < 0.05 to 0.001), with flows being higher on d 7, then decreasing on d 14, plateauing until d 35 and decreasing further on d 42. The concentrations of Trp, Cys, and Gly in the endogenous protein increased linearly (P < 0.01 to 0.001) with advancing age, whereas a linear decrease (P < 0.001) was noted for Lys. A quadratic influence (P < 0.05 to 0.001) was observed for the concentrations of Ile, Leu, Met, Val, and Asp. These changes in the endogenous protein profile may be attributed to variations in the contribution of endogenous sources with age but delineating the exact contribution of different sources is complicated. Overall, the current findings suggest that the basal ileal EAA flow is influenced by broiler age and age-specific EAA flows may need to be considered to standardize the AA digestibility.(c) 2021 The Author/sCC BY-NC-ND 4.0https://creativecommons.org/licenses/by-nc-nd/4.0/ageamino acidsbroilersendogenous flowAmino AcidsAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsChickensDietDigestionIleumMaleJournal article10.1016/j.psj.2021.1012691525-3171journal-article101269-https://www.ncbi.nlm.nih.gov/pubmed/34198102ARTN 101269S0032-5791(21)00303-5