Zhou LPoggesi SCasari Bariani GMittapalli RAdam P-MManzano MIonescu RE2019-04-102019-03-292019-06Biosensors (Basel), 2019, 9 (2)https://hdl.handle.net/10179/17789(c) The Author/sIn this study, stable gold nanoparticles (AuNPs) are fabricated for the first time on commercial ultrafine glass coverslips coated with gold thin layers (2 nm, 4 nm, 6 nm, and 8 nm) at 25 °C and annealed at high temperatures (350 °C, 450 °C, and 550 °C) on a hot plate for different periods of time. Such gold nanostructured coverslips were systematically tested via surface enhanced Raman spectroscopy (SERS) to identify their spectral performances in the presence of different concentrations of a model molecule, namely 1,2-bis-(4-pyridyl)-ethene (BPE). By using these SERS platforms, it is possible to detect BPE traces (10-12 M) in aqueous solutions in 120 s. The stability of SERS spectra over five weeks of thiol-DNA probe (2 µL) deposited on gold nano-structured coverslip is also reported.BPESERS on ultrafine solid supportsannealed gold nanostructuresglass coverslipsthiol-DNA probeBiosensing TechniquesDNA ProbesGlassGoldIndolesMetal NanoparticlesParticle SizeSpectrum Analysis, RamanSurface PropertiesTemperatureJournal article10.3390/bios90200534568782079-6374Massey_Dark0301 Analytical Chemistry0601 Biochemistry and Cell Biology