Poggesi SZhou LBariani GCMittapalli RManzano MIonescu REGuo PPortalone GPirri F2023-10-262023-10-262021-05-182023-10-262023-10-262021-05-18Poggesi S, Zhou L, Bariani GC, Mittapalli R, Manzano M, Ionescu RE. (2021). Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol. Crystals. 11. 5.https://mro.massey.ac.nz/handle/10179/68972A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/µL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.(c) 2021 The Author/sCC BYhttps://creativecommons.org/licenses/by/4.0/quartz crystal microbalancemulti-TEMs patterned crystalgenosensingBrettanomyces bruxellensisdrop and collect protocolJournal article10.3390/cryst110505622073-43522023-10-24journal-article