Giles JPerrott MRoe WDunowska M2016-042016-01-122016-04VIROLOGY, 2016, 491 pp. 20 - 260042-6822https://hdl.handle.net/10179/13214The objective of this study was to investigate a role of a recently discovered marsupial nidovirus in the development of a neurological disease, termed wobbly possum disease (WPD), in the Australian brushtail possum (Trichosurus vulpecula). Four possums received 1 mL of a standard inoculum that had been prepared from tissues of WPD-affected possums, 4 possums received 1.8 mL (1 × 10(6) TCID50) of a cell lysate from inoculated cultures, and 4 possums received 1 mL (× 10(7) TCID50) of a purified WPD isolate. All but one possum that received infectious inocula developed neurological disease and histopathological lesions characteristic for WPD. High levels of viral RNA were detected in livers from all possums that received infectious inocula, but not from control possums. Altogether, our data provide strong experimental evidence for the causative involvement of WPD virus in development of a neurological disease in infected animals.20 - 26ArterivirusWobbly possum diseaseWPDAustralian brushtail possumTrichosurus vulpeculaKoch's postulatesAetiologyJournal article10.1016/j.virol.2016.01.005259132Massey_Dark06 Biological Sciences07 Agricultural and Veterinary Sciences11 Medical and Health Sciences