Hawley HRRoberts CJFitzsimons HL2024-07-232024-07-232023-06-30Hawley HR, Roberts CJ, Fitzsimons HL. (2023). Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila.. MicroPubl Biol. 2023.2578-9430https://mro.massey.ac.nz/handle/10179/70280Spatial and temporal control of gene expression in Drosophila is essential in elucidating gene function. Spatial control is facilitated by the UAS/GAL4 system, and this can be coupled with additional adaptations for precise temporal control and fine tuning of gene expression levels. Here we directly compare the level of pan-neuronal transgene expression governed by nSyb-GAL4 and elav-GAL4, as well as mushroom body-specific expression alongside OK107-GAL4. We also compare the temporal modulation of gene expression in neurons with the auxin-inducible gene expression system (AGES) and temporal and regional gene expression targeting (TARGET) systems.(c) 2023 The Author/sCC BY 4.0https://creativecommons.org/licenses/by/4.0/Journal article10.17912/micropub.biology.0008852578-9430journal-articlehttps://www.ncbi.nlm.nih.gov/pubmed/37396791000885