Raudsepp AWilliams MAKJameson GBEnderlein J2023-11-062023-11-072022-02-022023-11-062023-11-072022-03-09Raudsepp A, Williams MAK, Jameson GB. (2022). Modeling multiple duplex DNA attachments in a force-extension experiment.. Biophys Rep (N Y). 2. 1. (pp. 100045-).2667-0747https://mro.massey.ac.nz/handle/10179/69068Optical tweezers-based DNA stretching often relies on tethering a single end-activated DNA molecule between optically manipulated end-binding beads. Measurement success can depend on DNA concentration. At lower DNA concentrations tethering is less common, and many trials may be required to observe a single-molecule stretch. At higher DNA concentrations tethering is more common; however, the resulting force-extensions observed are more complex and may vary from measurement to measurement. Typically these more complex results are attributed to the formation of multiple tethers between the beads; however, to date there does not appear to have been a critical examination of this hypothesis or the potential usefulness of such data. Here we examine stretches at a higher DNA concentration and use analysis and simulation to show how the more complex force-extensions observed can be understood in terms of multiple DNA attachments.(c) 2022 The Author/sCC BY-NC-ND 4.0https://creativecommons.org/licenses/by-nc-nd/4.0/Journal article10.1016/j.bpr.2022.1000452667-0747journal-article100045-https://www.ncbi.nlm.nih.gov/pubmed/36425083100045S2667-0747(22)00002-7