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dc.contributor.authorCornuault, VRGen_US
dc.contributor.authorBuffetto, Fen_US
dc.contributor.authorRydahl, MGen_US
dc.contributor.authorMarcus, SEen_US
dc.contributor.authorTorode, TAen_US
dc.contributor.authorXue, Jen_US
dc.contributor.authorCrepeau, MJen_US
dc.contributor.authorFaria-Blanc, Nen_US
dc.contributor.authorWillats, WGTen_US
dc.contributor.authorDupree, Pen_US
dc.contributor.authorRalet, MCen_US
dc.contributor.authorKnox, JPen_US
dc.date.available2015-07-25en_US
dc.date.issued2015-07-25en_US
dc.identifier.citationPlanta, 2015, 242 (6), pp. 1321 - 1334en_US
dc.identifier.issn0032-0935en_US
dc.description.abstractPlant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links.en_US
dc.format.extent1321 - 1334en_US
dc.languageenglishen_US
dc.publisherSpringer Verlagen_US
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605975/#__ffn_sectitleen_US
dc.rights© The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.subjectarabinogalactan-proteinen_US
dc.subjectcell wallen_US
dc.subjectglucuronoxylanen_US
dc.subjectpectinen_US
dc.subjectpolysaccharidesen_US
dc.subjectrhamnogalacturonan Ien_US
dc.titleMonoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell wallsen_US
dc.typeJournal Article
dc.citation.volume242en_US
dc.description.confidentialfalseen_US
dc.identifier.elements-id403108
dc.relation.isPartOfPlantaen_US
dc.citation.issue6en_US
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
dc.identifier.harvestedMassey_Dark
pubs.notesNot knownen_US
dc.subject.anzsrc0607 Plant Biologyen_US
dc.subject.anzsrc0703 Crop And Pasture Productionen_US


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