Browsing by Author "Bariani GC"

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    Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol
    (MDPI (Basel, Switzerland), 2021-05-18) Poggesi S; Zhou L; Bariani GC; Mittapalli R; Manzano M; Ionescu RE; Guo P; Portalone G; Pirri F
    A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/µL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.