Browsing by Author "Cox, Simon James Lethbridge"
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- ItemThe chloroplast genome of Arthropodium bifurcatum : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Biological Sciences, Massey University, Palmerston North, New Zealand(Massey University, 2010) Cox, Simon James LethbridgeThis thesis describes the application of high throughput (Illumina) short read sequencing and analyses to obtain the chloroplast genome sequence of Arthropodium bifurcatum and chloroplast genome markers for future testing of hypotheses that explain geographic distributions of Rengarenga – the name Maori give to species of Arthropodium in New Zealand. It has been proposed that A.cirratum was translocated from regions in the north of New Zealand to zones further south due to its value as a food crop. In order to develop markers to test this hypothesis, the chloroplast genome of the closely related A.bifurcatum was sequenced and annotated. A range of tools were used to handle the large quantities of data produced by the Illumina GAIIx. Programs included the de novo assembler Velvet, alignment tools BWA and Bowtie, the viewer Tablet and the quality control program SolexaQA. The A.bifurcatum genome was then used as a reference to align long range PCR products amplified from multiple accessions of A.cirratum and A.bifurcatum sampled from a range of geographic locations. From this alignment variable SNP markers were identified. Putative gene annotations for A.bifurcatum were compared to published chloroplast genomes from the Magnoliids and Monocot clades. Interesting similarities and differences have been detected and these have been discussed.
- ItemPlastid genes across the Great Divide : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Evolutionary Genetics/Bioinformatics, Institute of Fundamental Sciences, Massey University, Manawatu, New Zealand(Massey University, 2014) Cox, Simon James LethbridgeNearly all life that is visible to the naked eye is arguably a direct consequence of one or both endosymbiotic events that took place early in evolution and eventually resulted in the mitochondrion and the chloroplast. The timing of the mitochondrial endosymbiotic event weighs argument around the nature of LUCA (Last Universal Common Ancestor) being complex or simple and challenge the commonly taught view of bacteria being the first kingdom to emerge from the primordial state. The ancient metabolic pathways of amino acid and vitamin biosynthesis are examined and Ancestral Sequences constructed in order to discover the endosymbiotic signature within the nucleus of eukaryotes. Cyanobacterial and plant enzymes from these pathways are tracked as they cross from a prokaryotic coding environment to a eukaryotic one. If the eukaryote that took up the chloroplast ancestor was heterotrophic then it probably got some of its co-factors (vitamins) and essential amino acids from its diet. However, in order to become autotrophic it would have to be able to synthesise these amino acids and co-factors directly. The most likely source of these elements would have been the cyanobacterium; therefore cyanobacterial homologs should be found in the nuclear genome of plants. Ancestral Sequence Reconstruction (ASR) had a negligible effect on uncovering deeper endosymbiotic homologs. However ASR did confirm ancestral convergence between chloroplast and cyanobacterial homologs and between eukaryote nuclear genes and their cyanobacterial counterparts for vitamin and amino acid biosynthetic pathways. The results, all significant, show that the convergence is much stronger between organisms from the same coding environment (prokaryote [chloroplast] versus prokaryote [cyanobacteria]) than from different coding environments (eukaryote [nuclear] versus prokaryote [cyanobacteria]).