Browsing by Author "Currie, Alastair John"
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- ItemDifferentiating apple sports by pollen ultrastructure : a thesis presented in partial fulfilment of the requirements for the degree of Master of Horticultural Science at Massey University(Massey University, 1995) Currie, Alastair JohnCultivars are plants that form distinct, uniform and stable phenotypes. New cultivars can be protected by Plant Variety Rights (PVR) which allow the owner exclusive rights to the propagation and sale of the plant material. Current PVR identification methods for apple cultivars require detailed records of tree, flower and fruit characteristics to differentiate the new cultivars from known cultivars. This method is slow, expensive and unable to cope with the increasing numbers of sports. Biochemical identification methods such as isozymes, restriction fragment length polymerisation (RFLP), random amplified polymorphism DNAs (RAPD), and minisatellite probes, can quickly and objectively differentiate cultivars, but cannot differentiate apple sports. Previous research suggested that pollen ultrastructure could be an alternative method for plant identification. This thesis is concerned with the development of a technique to differentiate apple sports using pollen exine patterns. Scanning electron microscopy was used to capture images of the apple pollen grain and the exine surface. A digital image analysis algorithm was developed to extract quantitative data from the pollen grain dimensions and pore characteristics, and a Fast Fourier transform extracted quantitative data from the ridge patterns. Statistical methods were applied to the data to differentiate the sports. Pollen harvested from apple flowers in the spring were wider than pollen harvested from flowers forced out of season under artificial conditions. Significant differences between trees were found for pollen grain length:width ratio, percent pore coverage, pore area and pore length but further research is required. However, apple cultivars types 'Red Delicious' and 'Gala' were successfully differentiated by pore and pollen grain variables, and 'Aversang' and 'Ultrared' sports of 'Red Delicious', and 'Splenola' and 'Galalea' sports Of 'Gala' were successfully differentiated by exine ridge patterns and pollen grain measurements. Differentiation of apple sports by pollen requires further development but may be one of the only quick, objective identification methods that can differentiate sports. Sport differentiation would greatly aid PVR establishment and enforcement.
- ItemQuantitative genetics in apple (Malus x domestica (Borkh.)) breeding : fruit shape traits, genetic parameter estimation and breeding strategy development : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science (Breeding and Genetics) at Massey University, Palmerston North, New Zealand(Massey University, 2000) Currie, Alastair JohnThe aim of this thesis was to investigate some aspects of breeding for quantitative traits in apple. First, this study explored the measurement and inheritance of a complex quantitative trait (fruit shape). Fourier analysis was used to mathematically describe apple fruit shape in an objective manner and principal component analysis grouped the Fourier descriptors into meaningful shape traits. Heritabilities were estimated to determine genetically inherited shape traits, and genotype by environment interactions were estimated to determine the stability of the trait expression across environments. Fruit aspect ratio accounted for over 76% of the phenotypic variation in shape, fruit conicity for 6% and fruit squareness for only 2%. These traits had moderate to high narrow-sense heritabilities (0.79, 0.38 and 0.38 respectively), which indicated that individual selection would be efficient. High between-site genetic correlation (rA > 0.8), indicated low genotype-by-environment interaction and suggests breeding based at one site would be efficient for altering shape at other sites. A shape chart was constructed to enable these three apple shape traits to be evaluated quickly and accurately in the field, avoiding the need for image capture and Fourier analysis. Second, heritabilities and genetic correlations were estimated for a range of apple traits from a genetically broad-based apple breeding population of 71 families. Narrow-sense heritability was estimated at each of two sites and across sites for fruit acidity (0.17-0.22), fruit sugars (0.25-0.26), and tree growth habit (0.19-0.41). Higher heritabilities were estimated for fruit squareness (0.32-0.43), fruit conicity (0.32-0.46), powdery mildew incidence (0.40), vigour (0.28-0.62), fruit firmness (0.44-0.53), harvest time (0.66-0.82), leafing (0.60-0.83), fruit aspect ratio (0.74-0.89), flowering (0.92) and fruit size (0.90-1.01). Traits with a high narrow-sense heritability could be selected for efficiently with individual selection. High genetic correlation between sites (rA > 0.64) indicated low genotype by environment interaction and that breeding based at one site would be efficient for improving these traits at other sites. High positive genetic correlations between traits at site 1 were estimated for leafing with flowering (0.95), harvest time with fruit firmness (0.92), leafing with harvest time (0.69), and fruit acidity with fruit firmness (0.61). High negative genetic correlations were reported for vigour with fruit squareness (-0.82) and tree growth habit with leafing (-0.53). Rapid gains can be made with positively correlated traits, but high negatively correlated traits will make simultaneous gain in both traits difficult. Caution must be applied when interpreting genetic correlations between traits due to high standard errors and the possible influence of environmental interaction between traits (dependence) and linkage disequilibrium. Third, the traditional apple breeding strategy (mass selection) was compared to an option based on recurrent selection for general combining ability (RS-GCA). The excessively long generation interval of the traditional breeding strategy meant negligible gains were made. However, the traditional breeding strategy generated greater gains per generation than the RS-GCA strategy but at a greater cost in terms of eroding genetic diversity, thereby reducing the potential for long-term gain. The slower rate of gain in the RS-GCA was due to the slower rate of gain in the breeding population. However, the RS-GCA strategy made greater gains in the cultivars relative to the breeding population despite similar selection intensities due to better utilisation of the between-family genetic variance as well as the within-family genetic variance. Breeding strategies based on quantitative genetics have been applied with success to animal, crop and forest tree breeding, but only recently in apple breeding. It is clear that future breeding programmes would benefit from the application of quantitative genetic theory.