Browsing by Author "Guo M"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
    (2021-08-09) Hunziker L; Tarallo M; Gough K; Guo M; Hargreaves C; Loo TS; McDougal RL; Mesarich CH; Bradshaw RE
  • Thumbnail Image
    Item
    Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants.
    (7/10/2021) Hunziker L; Tarallo M; Gough K; Guo M; Hargreaves C; Loo TS; McDougal RL; Mesarich CH; Bradshaw RE
    Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclear whether any of these effectors are recognised by immune receptors to activate the host immune system. Such information is needed to identify and select disease resistance against D. septosporum in pines. We predicted and investigated apoplastic D. septosporum candidate effectors (DsCEs) using bioinformatics and plant-based experiments. We discovered DsCEs that trigger cell death in the angiosperm Nicotiana spp., indicative of a hypersensitive defence response and suggesting their recognition by immune receptors in non-host plants. In a first for foliar forest pathogens, we developed a novel protein infiltration method to show that tissue-cultured pine shoots can respond with a cell death response to a DsCE, as well as to a reference cell death-inducing protein. The conservation of responses across plant taxa suggests that knowledge of pathogen-angiosperm interactions may also be relevant to pathogen-gymnosperm interactions. These results contribute to our understanding of forest pathogens and may ultimately provide clues to disease immunity in both commercial and natural forests.
  • Thumbnail Image
    Item
    Attraction of the Indian Meal Moth Plodia interpunctella (Lepidoptera: Pyralidae) to Commercially Available Vegetable Oils: Implications in Integrated Pest Management
    (2024-09-06) Liu J; Yu Z; He XZ; Zhou G; Guo M; Deng J
    The Indian meal moth, Plodia interpunctella, poses a significant threat to global agricultural products. Although pheromone-based technologies show promise for P. interpunctella management, limitations such as single-sex targeting necessitate exploring complementary strategies. Vegetable- based oils represent a potential alternative but their efficacy, sex-specificity, and interaction with sex pheromones remain unclear. To address these questions, we first examined the attraction of P. interpunctella female and male adults to 10 commercially available vegetable oils in semi-field conditions. Sesame, olive, and blended oils were the most attractive, capturing significantly more adults compared to other oils. We then evaluated the effectiveness of these three attractive oils and their combinations with P. interpunctella sex pheromones in a grain warehouse. Traps baited with these oils captured significantly more females and males compared to control traps without attractants; however, the addition of sex pheromones did not improve male capture and significantly reduced female capture, suggesting an inhibitory effect. Finally, we demonstrated that female mating status (mated vs. virgin) did not influence the attractiveness of traps baited with these effective oils. Our findings highlight the potential of standalone traps baited with blended, olive, or sesame oil as a practical, economic, and effective management strategy for P. interpunctella in storage facilities.