Browsing by Author "Hansen, Nicolette Olivia"

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    Characterisation of malate-dependent mutants of the yeast Schizosaccharomyces malidevorans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University
    (Massey University, 1991) Hansen, Nicolette Olivia
    The phenotypes of several UV induced malate-dependent mutants of Schizosaccharomyces malidevorans were characterised and compared to the previously characterised malate-dependent strain, S. malidevorans #11. This strain uses less glucose than the wild type, has an extended life in liquid media, does not sporulate readily and turns indicator medium blue. The malate-dependent mutant strains were analysed for their fermentation characteristics, their complementation groups and their chromosomal patterns on a transverse alternating field electrophoresis apparatus (TAFE). The fermentation patterns of all of the strains were similar to #11. There appear to be three complementation groups involved in the malate-dependent phenotype. The TAFE patterns and subsequent Southern Hybridisation showed that the malate­ dependent mutants had a decreased mobility of chromosome II, while chromosomes I and III were not altered. The chromosome II alteration varied between the different malate-dependent mutants but fell into a small number of discrete patterns.