Browsing by Author "Jonker A"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium.
    (Nature Portfolio, 2022-10-20) Kumar S; Altermann E; Leahy SC; Jauregui R; Jonker A; Henderson G; Kittelmann S; Attwood GT; Kamke J; Waters SM; Patchett ML; Janssen PH
    Quinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH4) relative to their feed intake, but whether Quinella contributes to low CH4 emissions is not known. Here, we concentrate Quinella cells from sheep rumen contents, extract and sequence DNA, and reconstruct Quinella genomes that are >90% complete with as little as 0.20% contamination. Bioinformatic analyses of the encoded proteins indicate that lactate and propionate formation are major fermentation pathways. The presence of a gene encoding a potential uptake hydrogenase suggests that Quinella might be able to use free hydrogen (H2). None of the inferred metabolic pathways is predicted to produce H2, a major precursor of CH4, which is consistent with the lower CH4 emissions from those sheep with high abundances of this bacterium.
  • Thumbnail Image
    Item
    Methane emissions intensity in grazing dairy cows fed graded levels of concentrate pellets
    (Taylor and Francis Group, 2024-05-03) Bosher T; Della Rosa MM; Khan MA; Sneddon N; Donaghy D; Jonker A; Corner-Thomas R; Handcock R; Sneddon N
    The current New Zealand greenhouse gas inventory predictions assume that dairy cows consume pasture only, but the use of supplemental feeds, including concentrates, on New Zealand dairy farms has increased greatly in recent decades. The objective of this study was to evaluate the effect of feeding graded levels of concentrates on methane (CH4) emissions in lactating dairy cows within a pastoral system. Early lactation dairy cows (n = 72) were allocated (n = 18 per treatment) to receive 0, 2, 4 and 6 kg dry matter (DM) of treatment concentrates per day during milking. The cows grazed pasture ad libitum and CH4 emissions were measured in the paddocks using automated emissions monitoring systems called ‘GreenFeed’. Gross CH4 emissions (g/d) were similar for cows across the four dietary treatments, while CH4 emissions intensity (g/kg fat and protein corrected milk production (FPCM) and milk solids production) linearly decreased with increasing concentrate inclusion in the diet (P < 0.02). The CH4 intensity decreased linearly (r2 = 0.42) and quadratically (r2 = 0.53) with increasing FPCM production.
  • Thumbnail Image
    Item
    Plantain-mixed pasture collected in different climatic seasons produced less methane and ammonia than ryegrass–white clover pasture in vitro
    (CSIRO Publishing, 2025-06-23) Sivanandarajah K; Donaghy D; Molano G; Horne D; Kemp P; Navarrete S; Ramilan T; Pacheco D; Jonker A
    Context Plantain (PL) is recognised for reducing nitrate leaching and nitrous oxide emissions in pastoral systems. Evidence has shown that cows fed pure PL produced less methane (CH4) than cows fed ryegrass. However, it is unclear if the CH4 reduction can be achieved with PL in mixed pasture. Aim The study evaluated the in vitro rumen fermentation profiles of ryegrass–white clover (RWC) and medium-level PL (PLM, containing ~40% PL) pasture collected during different climatic seasons, to determine whether this inclusion level influences CH4 and rumen ammonia (NH3) production. Methods Substrates were selected from samples with various proportions of PL. Samples were categorised into three climatic seasons (i.e. spring, summer and autumn) and two pasture types (PLM and RWC). Representative samples for these scenarios were tested in an automated in vitro rumen batch culture system for gas, CH4 (mL/g DM) and NH3 (mM/g DM) production. Key results In summer samples, PLM produced approximately 8%, 14% and 19% less CH4 at 12 h, 24 h and potential CH4 production (PCH4), respectively. Although gas production (GP) was similar at 12 and 24 h, PLM had 13% lower potential GP than RWC (P < 0.05). In spring samples, PLM had approximately 11% greater GP and CH4 production at 12 h. For the autumn samples, GP and CH4 production were similar between PLM and RWC (P > 0.05). Net NH3 production from PLM substrates was significantly lower in spring (27%) and autumn (17%) samples, with no differences in summer, despite higher crude protein levels in the selected PLM. Conclusions Compared with RWC, PLM changed rumen fermentation parameters that could translate to potential environmental benefits: PLM produced less net NH3 in spring and autumn samples (27% and 17%, respectively), and up to 19% less CH4 production in summer samples. Implications Incorporating ~40% PL into RWC pasture showed a promising reduction of CH4 emissions and nitrogen losses in vitro. If the in vitro results translate to cows grazing pasture, this could offer greater environmental benefits with minimal input costs. In vitro results suggest that PLM’s potential to mitigate CH4 emissions can be influenced by seasonal variations in pasture quality compared with RWC. However, further animal studies are needed to fully comprehend the CH4 mitigation potential of this forage.