Browsing by Author "Panizzi L"

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A fermionic portal to a non-abelian dark sector
(Frontiers Media S.A., 2024-05-13) Belyaev A; Deandrea A; Moretti S; Panizzi L; Ross DA; Thongyoi N; Pasechnik R
We introduce a new class of renormalizable models for dark matter with a minimal particle content, consisting of a dark SU(2)D gauge sector connected to the standard model through a vector-like fermion mediator, not requiring a Higgs portal, in which a massive vector boson is the dark matter candidate. These models are labeled fermion portal vector dark matter (FPVDM). Multiple realizations are possible, depending on the properties of the vector-like partner and scalar potential. One example is discussed in detail. Fermion portal vector dark matter models have a large number of applications in collider and non-collider experiments, with their phenomenology depending on the mediator sector.
Efficacy of needle and endoscopic lavage on the recuperation of microspheres from the adult equine metacarpo−/metatarsophalangeal joint and digital flexor tendon sheath
(Wiley Periodicals LLC on behalf of American College of Veterinary Surgeons, 2025-06-25) Beggan CP; Panizzi L; Oliver LJ
Objectives: To measure microsphere recovery following needle-through-and-through lavage (NTAT) of the metacarpo−/metatarsophalangeal joint (fetlock) and digital flexor tendon sheath (DFTS) compared to endoscopic lavage (EL). Study design: Ex vivo experimental study. Animals: Adult equine cadavers immediately following euthanasia (n = 10). Methods: Colored 15 μm microspheres (2 million) were injected into fetlock joints and DFTS. Synovial structures were assigned to NTAT or EL groups. Each lavage was performed using 5 L of 0.9% NaCl, sequentially collecting egress fluid for microsphere quantification. Recovery was compared using a full-factorial general linear model. Results: There was a significant effect of the liter of egress fluid and microsphere recovery in both fetlocks (p <.01) and DFTS (p <.01), with most microspheres recovered in the first 2 L (79%–83%) for both techniques. More microspheres were recovered in the first liter using NTAT than EL (p <.01) in both fetlocks (659 883 ± 20 820 vs. 567 601 ± 24 452) and DFTS (644 341 ± 17 460 vs. 550 637 ± 38 022). No difference in total recovered microspheres was observed between NTAT lavage of fetlock (981 600 ± 46 839) and DFTS (957 419 ± 45 729) across 5 L (p =.88). Conclusion: Needle-through-and-through lavage was more effective than EL at recovering microspheres in the first liter from cadaveric equine fetlock joints and DFTS. Both techniques demonstrated comparable efficacy between fetlock and DFTS in microsphere recovery following increased lavage volumes. Clinical significance: Needle-through-and-through lavage (NTAT) is a viable alternative for suspected synovial contamination when EL is delayed or not feasible. This study does not evaluate NTAT's efficacy for treating established sepsis or removing pannus/foreign bodies.
Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis
(Elsevier Ltd on behalf of Osteoarthritis Research Society International (OARSI), 2022-12) Panizzi L; Vignes M; Dittmer KE; Waterland MR; Rogers CW; Sano H; McIlwraith CW; Pemberton S; Owen M; Riley CB
OBJECTIVE: to determine the accuracy of infrared (IR)-based serum biomarker profiling to differentiate horses with early inflammatory changes associated with a traumatically induced model of equine carpal osteoarthritis (OA) from controls. METHOD: unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder served as sham operated controls. Serum samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63 from both groups. Films of dried serum were created, and IR absorbance spectra acquired. Following pre-processing, partial least squares discriminant analysis (PLSDA) and principal component analysis (PCA) were used to assess group and time differences and generate predictive models for wavenumber ranges 1300-1800 cm-1 and 2600-3700 cm-1. RESULTS: the overall correct classification rate when classifying samples by group (OA or Sham) was 52.7% (s.d. = 12.8%), while it was 94.0% (s.d. = 1.4%) by sampling Day. The correct classification results by group-sampling Day combinations with pre-intervention serum (Day 0) was 50.5% (s.d. = 21.7%). CONCLUSION: with the current approach IR spectroscopic analysis could not differentiate serum of horses with induced carpal OA from that of controls. The high classification rate obtained by Day of sampling may reflect the effect of exercise on the biomarker profile. A longer study period (advanced disease) or naturally occurring disease may provide further information on the suitability of this technique in horses.
Infrared Spectroscopy of Synovial Fluid Shows Accuracy as an Early Biomarker in an Equine Model of Traumatic Osteoarthritis
(MDPI (Basel, Switzerland), 2024-03-22) Panizzi L; Vignes M; Dittmer KE; Waterland MR; Rogers CW; Sano H; McIlwraith CW; Riley CB; Kaneps AJ
Osteoarthritis is a leading cause of lameness and joint disease in horses. A simple, economical, and accurate diagnostic test is required for routine screening for OA. This study aimed to evaluate infrared (IR)-based synovial fluid biomarker profiling to detect early changes associated with a traumatically induced model of equine carpal osteoarthritis (OA). Unilateral carpal OA was induced arthroscopically in 9 of 17 healthy thoroughbred fillies; the remainder served as Sham-operated controls. The median age of both groups was 2 years. Synovial fluid (SF) was obtained before surgical induction of OA (Day 0) and weekly until Day 63. IR absorbance spectra were acquired from dried SF films. Following spectral pre-processing, predictive models using random forests were used to differentiate OA, Sham, and Control samples. The accuracy for distinguishing between OA and any other joint group was 80%. The classification accuracy by sampling day was 87%. For paired classification tasks, the accuracies by joint were 75% for OA vs. OA Control and 70% for OA vs. Sham. The accuracy for separating horses by group (OA vs. Sham) was 68%. In conclusion, SF IR spectroscopy accurately discriminates traumatically induced OA joints from controls.
Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses
(MDPI (Basel, Switzerland), 2023-03-14) Panizzi L; Dittmer KE; Vignes M; Doucet JS; Gedye K; Waterland MR; Rogers CW; Sano H; McIlwraith CW; Riley CB; Zucca E
Biomarkers for osteoarthritis (OA) in horses have been extensively investigated, but translation into clinical use has been limited due to cost, limited sensitivity, and practicality. Identifying novel biomarkers that overcome these limitations could facilitate early diagnosis and therapy. This study aimed to compare the concentrations of synovial fluid (SF) and plasma cell-free DNA (cfDNA) over time in control horses with those with induced carpal OA. Following an established model, unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder were sham-operated controls. Synovial fluid and plasma samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63, and cfDNA concentrations were determined using fluorometry. The SF cfDNA concentrations were significantly higher for OA joints than for sham-operated joints on Days 28 (median 1430 μg/L and 631 μg/L, respectively, p = 0.017) and 63 (median 1537 μg/L and 606 μg/L, respectively, p = 0.021). There were no significant differences in plasma cfDNA between the OA and the sham groups after induction of carpal OA. Plasma cfDNA measurement is not sufficiently sensitive for diagnostic purposes in this induced model of OA. Synovial fluid cfDNA measurement may be used as a biomarker to monitor early disease progression in horses with OA.
Surgical treatment of a five-structure dentigerous cyst in a Warmblood filly
(John Wiley and Sons Ltd on behalf of the British Equine Veterinary Association, 2023-05) Howell A; Panizzi L; Simpson E
This article describes a unilateral extracranial dentigerous cyst containing five dental structures in a 3-year-old Warmblood filly that caused drainage from below the left pinna and behavioural changes due to pain. Initial diagnosis was made using radiographs and further diagnostic imaging in the form of computed tomography was used for effective surgical planning. This highlighted the proximity of the cyst to the calvarium. All the structures were surgically removed using an osteotome and mallet. During surgery, a defect was made in the calvarium leading to a small haemorrhage, which was successfully controlled. The external auditory canal was accidentally entered during the procedure; closure was achieved and no postoperative infection was experienced. The structures were histologically confirmed to be dental in origin. On telephone and photographic follow-up, the horse showed excellent cosmetic results, with no neurological or motor deficits and complete resolution of clinical signs.