Browsing by Author "Soe, Aye Kyawt"
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- ItemCoccidia (Protozoa: Apicomplexa) of the domesticated goat Capra hircus in New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in veterinary science at Massey University(Massey University, 1989) Soe, Aye KyawtThe literature on the history of the identification of Eimeria species infecting domesticated goats, and their morphological characteristics, the general life cycle of coccidia and epidemiology of infections, together with clinical signs, diagnosis and treatment is reviewed. In total 13 Eimeria species which are generally considered valid have been described from goats but relatively little has been published on their biology or significance. Studies on the identification and seasonal prevalence of Eimeria species infecting domesticated goats were conducted on three farms in the Palmerston North area of New Zealand from August 1987 to August 1988. On one farm (Old West Road), 20 kids & 20 adult Saanen goats were sampled at 14 day intervals and monthly intervals, respectively; on a second farm (Ballantrae) 17 kids and 20 adult New Zealand 'feral' type were sampled at monthly intervals; on a third farm (Kimbolton) 23 Angora kids were sampled at 14 day intervals. Faecal samples collected directly from the rectum were used for oocyst counts and to provide oocysts for sporulation for identification of species. More than 98% of the faecal samples from each group of kids and adults contained coccidial oocysts. Mixed infections were the rule, 59% of the faecal samples contained 6-8 species. The Eimeria species identified in this study were: ten previously described species - E. christenseni, E. tunisiensis, E. jolchejevi s.s., E. arloingi, E. hirci, E. caprina, E. caprovina, E. apsheronica, E. ninakohlyakimovae and E. alijevi; two others whose species status is uncertain - temporarily designated E. jolchejevi 'large form' and E. hirci 'small form'; three previously undescribed species - temporarily designated E. nt, E. n2 and E. n3. The morphological characteristics of sporulated oocysts of the Eimeria species found in the present survey are described in detail and illustrated by microphotographs and schematic diagrams. Statistical analysis of oocyst and sporocyst dimensions of these species and, where necessary for differentiation of species, statistical comparisons are also given. In addition to these species, a single oocyst of E. punctata which was not recorded during the study period, was found later later in a pooled sample kept as reference material. E. jolchejevi 'large form' had many of the characteristics of E. jolchejevi as described in the literature but differed in size and shape from it. Comparisons of the large form with published data indicate that it represents a previously overlooked species infecting goats and that there may be an equivalent species in sheep. E. hirci oocysts observed did not differ from published descriptions but analysis showed that they were divisible into two distinct groups on the basis of size and shape. These may represent seperate species but further investigation is needed to verify this. The species designated E. n1, E. n2 and E. n3 are shown to be clearly distinguishable from previously described species from goats. E. n1 may represent the equivalent of E. weybridgensis from sheep but the other two species do not appear to have an ovine equivalent. Further work is needed to confirm their species status and investigate their biology. Kids less than 1 year old had considerably higher oocyst counts than adults. Mean oocyst counts were at their highest from 2-6 months of age but they tended to decrease with time and from May on were at relatively low levels. The seasonal patterns of oocyst counts in the groups of kids differed between farms. This was associated with different management systems and breeds. The highest oocyst counts occurred in Saanen kids raised on milk-replacer in a heavily contaminated pen; the predominant species were E. christensen; and E. arfoingi. Counts were lower in Angora kids reared on milk-replacer in pens that were cleaned daily and which opened onto a small paddock. Counts were lowest in 'feral' kids reared by natural suckling at pasture. In adults, on one farm there was no detectable seasonal trend in oocyst counts and on the other there was a tendency for mean counts to rise from December on. On both farms there were, in addition, some short-term fluctuations in mean counts with peaks associated with high counts in a few individuals. Seasonal variations in the occurrence of individual Eimeria species were examined by considering the oocyst counts for each species, the percentage of the total oocyst counts represented and the prevalence of positive faecal samples. The mean percentages of total oocyst counts represented by each species over the whole year were compared. E. arloingi was found to be the most predominant species. Other species which were dominant in the coccidial population were E. hirci and E. n2. The seasonal patterns differed between species although the patterns on the different farms were, on the whole, very similar. This indicates substantial differences between species in host-parasite relationships and it is suggested that these may chiefly involve the prolificacy and immunogenicity of the various species. Further work is needed to investigate this. All of the species recorded were found in all the groups of goats examined. The sporulation of E. christenseni oocysts at various constant temperatures was examined. The log temperature:log development time relationship yielded a correlation coefficient of r = -0.99. The time required for 90% of the oocysts to complete each development stage was taken as the endpoint. Sporulation was completed in 7 days at 27°C, in 10 days at 20°C, in 11 days at 15°C, 14 days at 10°C and in 32 days at 4°C.
- ItemLarval paralysis factor of sheep : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand(Massey University, 2008) Soe, Aye KyawtThis study aimed to identify the origin and molecular nature of larval paralysis factor (LPF), an uncharacterised natural anthelmintic agent(s) known to be secreted by cells in the small intestine of nematode-parasite-immune sheep. The study first confirmed previous findings that gut mucus and small intestinal mucosal cell culture supernatants (CCSs) from parasite-immune sheep contain LPF. An in vitro larval migration inhibition (LMI) assay showed that LPF is active against numerous nematode parasite larvae of ruminants and adults of Trichostrongylus colubriformis. Lamina propria cells (LPCs) were a richer source of LPF than epithelial cells, and release of the factor was specifically triggered by parasite larval antigens. A series of trials was performed to optimise LPF production in vitro. LPF production was highest when LPCs were selectively extracted from the first three metres of small intestine of resistant-line or hyperimmune parasitized sheep three days after oral challenge with T. colubriformis larvae. LPF release in vitro appeared to be related to the percentage of eosinophils present in cell cultures, but not with mucosal mast cells or globule leucocytes. Subsequent in vitro experiments showed that mucus glycoproteins released from goblet cells enhanced and sustained the activity of LPF, which may point to a previously unrecognised effector link between goblet cell hyperplasia and mucosal immune responses to gut nematode parasites. Sequential purification and molecular analysis of LPF showed that the active agent has a molecular mass less than 1kDa, it is polar and heat and enzyme resistant. Further purification using solid phase extraction and HPLC established that LPF did not bind either C18 or ion exchangers, but LPF activity in the flow through from these sorbents was retained on aminopropyl HPLC columns and eluted as a single active peak after flushing with acetonitrile/water mixture (70:30). This peak, analysed by LC-MS, comprised three main compounds of interest: a UV absorbing compound at 254nm with no MS data, and two non UV absorbing compounds at 254nm: one was a component of m/z 104 (ESI+ve) and the other was detected at m/z 113 (ESI-ve).