Browsing by Author "Sofkova-Bobcheva S"
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- ItemBlueberry firmness - A review of the textural and mechanical properties used in quality evaluations(Elsevier B.V, 2022-10-01) Rivera S; Giongo L; Cappai F; Kerckhoffs H; Sofkova-Bobcheva S; Hutchins D; East AFirmness is an important parameter for fresh blueberries as it influences the quality perceived by consumers and postharvest storage potential. However, the blueberry research community has not yet identified a universal standard method that can evaluate firmness for quality purposes. Different mechanical tests have been considered, offering different perspectives on this quality trait. This review summarises the most common methods previously used to evaluate textural and mechanical properties of fresh blueberries as influenced by pre- and postharvest factors. In addition, this review intends to assist the blueberry research community and commercial supply chain when selecting suitable methods to measure blueberry firmness as a fruit quality response. Different research initiatives to develop, optimize or standardise instrumental methods to assess blueberry firmness and relate to consumer sensory perception are reviewed. Mechanical parameters obtained by compression tests are the most previously used techniques to evaluate the influence of genotype, maturity, calcium, and postharvest management on blueberry firmness or to relate to sensory descriptors. However, standardising operational settings (e.g., compression distance, loading speed, and calculation procedures) is required to make results comparable across data collection conditions. Whether other mechanical test methods such as penetration or a combination of tests can better characterise blueberry quality or the relationship with consumer acceptance remains unknown and is worth studying.
- ItemCharacterization of the Volatile Profiles of Six Industrial Hemp (Cannabis sativa L.) Cultivars(American Society of Agronomy, 27/10/2022) Sofkova-Bobcheva SVolatile organic compounds (VOCs) play an important role in plant ecology and can be useful in pest management. This work characterises, for the first time, the VOC emissions of six industrial hemp (Cannabis sativa L.) cultivars grown in New Zealand: CFX-2, CRS-1, Ferimon 12, Katani, Futura 75, and Finola. Volatiles emitted from flowers and foliage of eight-week-old plants were collected using a dynamic headspace sampling method and analysed using gas chromatography coupled to mass spectrometry. We assessed the effect of cultivar, sex (monoecious, male, and female), and site (i.e., two sites differing in soil types, maintained under irrigation and rain-fed conditions) on VOC emissions. Thirty-five volatile compounds were tentatively identified from the headspace samples of hemp plants, but none of the cultivars emitted all 35 compounds. β-Myrcene was the most abundant compound in most cultivars. Overall, there was a significant effect of sex, and the interaction of sex and cultivar on the volatile profiles, but no effect of site. Female plants typically emitted more volatiles than their male counterparts and monoecious cultivars. The main compounds driving the difference between cultivars and sexes were (Z)- and (E)-β-ocimene. We hypothesize that differences in emission emerged as a defence strategy to protect costly female flowers from herbivores (since C. sativa is wind pollinated), but this hypothesis needs further testing. We recommend additional studies exploring how biotic and abiotic factors influence hemp VOC emissions, changes in VOCs throughout the crop cycle, the role of VOCs in plant-insect interactions and their use in pest management.
- ItemComparing vegetative growth patterns of cultivated (Daucus carota L. subsp. sativus) and wild carrots (Daucus carota L. subsp. carota) to eliminate genetic contamination from weed to crop(Elsevier B.V., 2024-05-20) Godwin A; Pieralli S; Sofkova-Bobcheva S; Ward A; McGill CWild carrot is a problematic weed that can threaten the genetic purity of cultivated carrots by hybridization. Wild carrots must be controlled before flowering to avoid the undesirable crossing with cultivated carrots. Understanding wild carrot's vegetative growth pattern helps formulate sustainable weed management practices. However, little is known about the vegetative growth patterns of wild and cultivated carrots. A pot experiment was carried out to compare and model the vegetative growth pattern of different morphological traits in both wild and cultivated carrots. This study was executed in a glasshouse located in Palmerston North, New Zealand. A factorial randomized complete block design (RCBD) with two factors and four replications was used. The first factor was assigned to the carrot genotype (cultivated and wild) and the second factor to length of juvenile stages (12-weeks, 8-weeks, and 4-weeks). Plant height, leaf number, shoot fresh and dry weight, root fresh and dry weight, root diameter and root length were measured. Data were analyzed using analysis of variance (ANOVA), principal component analysis (PCA), correlation, and regression analysis. At the 8-week juvenile stage (9–11 leaves stage), wild carrot's shoot and root characteristics exhibited rapid growth. Correlation analysis indicated positive and significant (p < 0.05) correlations between above and below-ground morphological traits. PCA showed that morphological characteristics, except plant height, can be used to distinguish wild and cultivated carrots. To predict the vegetative growth pattern of most of the morphological traits of wild and cultivated carrots, power regression models were selected based on higher R2 and adj-R2 values and lower values of RMSE, AIC and BIC. The study showed wild carrots grew more quickly than cultivated carrots during the vegetative phase. It is recommended that appropriate weed management practices, such as hoeing, tilling, hand pulling, or herbicide spraying, be implemented before wild carrot leaf stages 9–11.
- ItemData of texture profile analysis performed by different input settings on stored ‘Nui’ and ‘Rahi’ blueberries(Elsevier Inc, 2021-10) Rivera S; Kerckhoffs H; Sofkova-Bobcheva S; Hutchins D; East ATexture Profile Analysis is a well-established method for assessing mechanical properties of horticultural food products and consists of two compression cycles on a repeated motion to a given strain using a flat surface probe (i.e., compression plate). Input settings of target deformation (strain%) and duration (s) between compression cycles utilized for Texture Profile Analysis could influence output mechanical properties. The article provides data related to the ability of different Texture Profile Analysis operational settings to enable the separation of blueberries with variable mechanical properties. To create variable mechanical parameters of ‘Nui’ and ‘Rahi’ blueberries, fruit was stored in four relative humidity for 21 d at 4°C. For each storage humidity, mechanical properties of hardness (BH, N), hardness slope (BHS, kN m−1), apparent modulus of elasticity (E, MPa), and resilience (BR, -) were determined by utilizing two strain (15% or 30% of berry equatorial height). Meanwhile, mechanical parameters of cohesiveness (BCo, -), and springiness (BSp, -) were obtained by utilizing the combination of two strain (15% or 30%) and two duration between cycles (2 s and 10 s) as TPA operational settings. The statistical evaluation was conducted by one-way ANOVA, and the means of each storage humidity were separated according to the Tukey-HSD test (P = 0.05). The data presented in this article was used to select the Texture Profile Analysis operational settings utilized in the article entitled “Influence of water loss on mechanical properties of stored blueberries” Rivera et al. [1].
- ItemInfluence of water loss on mechanical properties of stored blueberries(Elsevier, 27/02/2021) Rivera S; Kerckhoffs H; Sofkova-Bobcheva S; Hutchins D; East AMoisture loss is considered a main cause of blueberry softening during postharvest storage. However, the causal relationship between softening and water loss has only previously been described by force to 1 mm compression. This study was performed to identify suitable instrumental tests that allow the separation of blueberries with different water loss values during storage. Mechanical properties were measured by double compression (Texture Profile Analysis) and puncture test. Variability on blueberry mechanical properties was created by regulating storage humidity and consequently water loss. As water loss increases during storage, hardness slope (slope of a straight line drawn between the trigger force of 0.06 N and the force at 15 % strain) obtained by the compression test reduces, and the displacement at berry skin break obtained by puncture test, increases. Therefore, these parameters can be potentially used to quantify mechanical changes in stored blueberries.
- ItemMoringa oleifera L.: A Potential Plant for Greenhouse Gas Mitigation in Temperate Agriculture Systems(Versita, 28/07/2022) Sofkova-Bobcheva SThe earth’s climate is changing because of the increase in greenhouse gas (GHG) concentration, to which livestock is a major contributor. Methane produced from cattle can be reduced by using high quality forages. This study compared the GHG produced from M. oleifera in an artificial ruminant system with two high quality pasture species, ryegrass and white clover. Methane and total gas production were measured using an in vitro batch culture system. A preliminary screening using oven dried M. oleifera planted in field and greenhouse, and a main experiment using six provenances of M. oleifera, a composite sample and M. oleifera leaves from greenhouse was undertaken. Both experiments compared the M. oleifera from different sources with high quality ryegrass and white clover. Real time gas production was recorded for 48 h, total gas production, methane analysed at 12 and 24 h. Short chain fatty acids concentration were also determined at the end of the fermentation. Preliminary results showed that M. oleifera leaves grown in field and greenhouse have lower gas and methane production compared with ryegrass, but similar to white clover. The differences were driven by a high production of propionic and butyric acids. The six M. oleifera provenances also produced less methane than ryegrass but were similar to white clover at 12 and 24 h after the start of fermentation. M. oleifera fermented faster than ryegrass or white clover. Hydrogen production from fermentation of M. oleifera might not have been diverted to methane production but removed by other compounds. In vitro fermentation showed differences in methane production across provenances. This suggests that it may be possible to select for low methane genotypes.
- ItemStructure-Function Characterisation of Eop1 Effectors from the Erwinia-Pantoea Clade Reveals They May Acetylate Their Defence Target through a Catalytic Dyad.(MDPI (Basel, Switzerland), 2023-09-28) Tomar V; Rikkerink EHA; Song J; Sofkova-Bobcheva S; Bus VGM; Bhadauria V; Zhao WThe YopJ group of acetylating effectors from phytopathogens of the genera Pseudomonas and Ralstonia have been widely studied to understand how they modify and suppress their host defence targets. In contrast, studies on a related group of effectors, the Eop1 group, lag far behind. Members of the Eop1 group are widely present in the Erwinia-Pantoea clade of Gram-negative bacteria, which contains phytopathogens, non-pathogens and potential biocontrol agents, implying that they may play an important role in agroecological or pathological adaptations. The lack of research in this group of YopJ effectors has left a significant knowledge gap in their functioning and role. For the first time, we perform a comparative analysis combining AlphaFold modelling, in planta transient expressions and targeted mutational analyses of the Eop1 group effectors from the Erwinia-Pantoea clade, to help elucidate their likely activity and mechanism(s). This integrated study revealed several new findings, including putative binding sites for inositol hexakisphosphate and acetyl coenzyme A and newly postulated target-binding domains, and raises questions about whether these effectors function through a catalytic triad mechanism. The results imply that some Eop1s may use a catalytic dyad acetylation mechanism that we found could be promoted by the electronegative environment around the active site.
- ItemYield and tuber quality variability in commercial potato cultivars under abiotic stress in New Zealand.(2018) Siano A; Kerckhoffs LHJ; Roskruge N; Sofkova-Bobcheva S