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Item Environmental drivers of antimicrobial resistance – cadmium contamination & antibiotic resistance in soil samples from a rural airstrip.(2023-12-05) Heydari A; Kim N; Biggs P; Horswell J; Gielen G; Siggins A; Bromhead C; Palmer BEnvironmental contamination with both inorganic and organic compounds is a growing problem globally. In this study we investigated links between heavy metal contamination of soil and selection for antibiotic resistance in soil bacteria. Soil samples taken at 10 m intervals along the length of a 70 m transect of a rural airstrip used for aerial topdressing located in Belmont Regional Park near Wellington were analysed for heavy metal content and resistance profiles of heterotrophic bacteria cultured were characterised. A gradient of cadmium contamination (a known contaminant of superphosphate fertiliser) ranging from 1.14 to 7.20 mg kg-1 of dry soil was detected in the samples. Total bacterial counts were significantly reduced at the most heavily contaminated subsites, with >60% of isolates resistant to 0.01 mM CdCl2. The ratio of antibiotic resistant isolates to total CFU was significantly higher at the most contaminated compared to the least contaminated subsite for five common antibiotics. Metagenomic analysis of total DNA from three subsites showed significantly different profiles at all taxonomic levels. This suggests environmental contamination with heavy metals may be a significant and under-appreciated driver of selection for antimicrobial resistance.Item Differential gene expression in earthworms exposed to different concentrations of Cadmium(Conference organisers, 29/06/2018) Thunders M; Dharmadasa P; Kim NIntroduction. Cadmium (Cd) is a common contaminant found in many foods, accumulates throughout life and, in high doses, is a significant health hazard for humans. Cadmium is of interest because its concentration in agricultural soils has become elevated over time; with dietary intake in Western countries often close to tolerable limits. Analysis of change in gene expression in response to environmental stressors could potentially aid in risk assessment and biomarker development for human studies. Eisenia fetida are commonly used in ecotoxicological studies. Object. To look at the impact of cadmium concentration on gene expression through comparative analysis of transcriptome data from E. fetida exposed to zero, low, medium and high doses of cadmium. Methods. Artificial soil was spiked with three different Cd solutions [...]Item Consequences of Cadmium exposure on growth and reproduction across three generations of earthworm(Conference organisers, 29/06/2018) Dharmadasa P; Kim N; Li Y; Thunders MHeavy metal pollution disturbs the soil ecosystem by negatively affecting soil fauna and flora. In term of biomass and activity Annelids are a very important part of the soil invertebrate community. They are one of the first organisms affected by heavy metal contamination in soil and as such are good model organisms for assessing soil contamination. The aim of this research is to observe how Cd impacts on health and reproduction in three consecutive generations of E. fetida. [...]