Massey Documents by Type

Permanent URI for this communityhttps://mro.massey.ac.nz/handle/10179/294

Browse

Filters

2022 - 20254

Settings

Start date: 2022Subject: search.filters.subject.transformation

Search Results

Now showing 1 - 4 of 4
  • Thumbnail Image
    Item
    Simple and Efficient Transformation and Gene Editing of Marchantia polymorpha Spores
    (Wiley Periodicals LLC, 2025-05-27) Yorker RM; Deroles SC; Zhou Y; Tate JA; Davies KM; Albert NW
    Marchantia polymorpha (Marchantia) has become a model species for liverwort studies, owing to its rapid growth in vitro, ease of propagation, simple genetics, small genome, haploid-dominant life cycle, and because it is amenable to genetic transformation. Efficient transformation provides a foundation for many molecular and genetic analyses. The protocol described here is a simple and robust procedure for transforming Marchantia for a variety of applications, including gene overexpression and CRISPR genome editing. This simplified Agrobacterium tumefaciens-mediated transformation protocol targets spores, using common Agrobacterium strains GV3101 or EHA105, and overcomes challenges experienced in other methods. Spores are sterilized and distributed over sterile filter papers, which effectively retain spores and regenerating spores (known as sporelings). This approach enables the sporelings to be transferred to different agar growth media at different stages of transformation. A critical feature is preculturing the spores with acetosyringone (AS) prior to co-cultivation with Agrobacterium. This treatment profoundly enhances the transformation rate, particularly for Agrobacterium strain GV3101. GV3101 is preferred for its rapid growth rate, simple transformation, and lack of a recombinase (recA), stabilizing plasmids. The protocol is suitable for transforming Marchantia with constructs for CRISPR gene editing. Editing efficiency can be increased by introducing a heat-shock treatment during the transformation procedure, which increases the proportion of plants with larger edited sectors, facilitating mutant identification and propagation. Constructs and strategies for both overexpression and multiplex genome editing with sgRNA arrays using new and existing vectors are described. Using this spore transformation protocol for CRISPR gene editing, we routinely achieve 60% to 70% mutation rates, significantly reducing the effort required to generate and isolate mutants for functional analyses.
  • Thumbnail Image
    Item
    Co-creating sustainability: Transformative power of the brand
    (Taylor and Francis Group, 2024-07-17) Palakshappa N; Bulmer S; Dodds S
    We explore marketing activity at the micro level as it contributes to the co-creation of sustainability and leads to transformational shifts towards sustainable futures. Utilising a value co-creation lens our study implements a novel four phase case research process. Three sustainable fashion brands are analysed via brand-generated content on their Instagram accounts, through interviews with thirty self-confessed brand enthusiasts and using consumer focus groups. Analysis provides insights into how sustainability is co-created between brands and consumers demonstrating that marketing and brands have the power to harness sustainability and instigate change. The paper contributes an innovative ‘Co-creating sustainable futures’ framework providing a platform to implement marketing activity and future research by marketing/brand managers and researchers committed to sustainability and initiating transformation towards sustainable futures.
  • Thumbnail Image
    Item
    The impact of ChatGPT on teaching and learning in higher education: Challenges, opportunities, and future scope
    (IGI Global, 2024-04-01) Li M; Khosrow-Pour MDBA
    The integration of OpenAI's ChatGPT is reshaping higher education by transforming teaching and learning dynamics. This article delves into ChatGPT's impact, exploring opportunities, challenges, and future potential. ChatGPT's deployment in higher education offers interactive and adaptive classrooms, enabling personalized learning experiences. Educators use ChatGPT to enhance engagement, critical thinking, and tailor content, fostering innovative teaching. However, integrating ChatGPT also introduces challenges, including plagiarism detection concerns due to AI-generated assignments and potential impacts on writing skills and independent thinking. Addressing misinformation risks from AI content requires responsible usage guidelines. Looking forward, ChatGPT holds promise in higher education, as AI-enhanced collaborative classrooms redefine teaching. The symbiosis of ChatGPT with human instructors enhances effectiveness, providing real-time insights and boosting student engagement.
  • Thumbnail Image
    Item
    The tragedy of climate change science
    (Taylor and Francis Group, 2022-11) Glavovic BC; Smith TF; White I
    The science-society contract is broken. The climate is changing. Science demonstrates why this is occurring, that it is getting worse, the implications for human well-being and social-ecological systems, and substantiates action. Governments agree that the science is settled. The tragedy of climate change science is that at the same time as compelling evidence is gathered, fresh warnings issued, and novel methodologies developed, indicators of adverse global change rise year upon year. Meanwhile, global responses to Covid-19 have shown that even emergent scientific knowledge can bolster radical government action. We explore three options for the climate change science community. We find that two options are untenable and one is unpalatable. Given the urgency and criticality of climate change, we argue the time has come for scientists to agree to a moratorium on climate change research as a means to first expose, then renegotiate, the broken science-society contract.