Massey Documents by Type
Permanent URI for this communityhttps://mro.massey.ac.nz/handle/10179/294
Browse
2 results
Search Results
Item Identification and functional characterisation of glycoside hydrolases from the kauri dieback pathogen, Phytophthora agathidicida : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy (PhD) in Plant Science at Massey University, Manawatū, New Zealand(Massey University, 2022) Bradley, Ellie LynnThe survival of kauri, an ancient conifer species endemic to New Zealand, is currently threatened by kauri dieback disease, caused by the oomycete plant pathogen Phytophthora agathidicida. As P. agathidicida continues to spread throughout kauri forests in the northern North Island of New Zealand, encouraging research has indicated there may be natural tolerance to the disease within the kauri population. This resistance is likely governed, in part, by the plant immune system, which is activated upon recognition of pathogen invasion patterns such as microbe-associated molecular patterns (MAMPs), damage-associated molecular patterns (DAMPs), and effectors (virulence factors required for host colonisation), which are recognised at the plant cell surface by plant immune receptors. To better understand how P. agathidicida interacts with its plant host on a molecular level, pathogenproduced proteinaceous invasion patterns need to be identified and characterized to aid in the identification of cognate immune receptors in the plant host, which may be involved in activation of the plant immune system. As the role of glycoside hydrolase (GH) proteins in virulence and pathogenicity of fungal and oomycete plant pathogens is well established (Chapter two), an effectoromics approach was used to identify six P. agathidicida GH12 proteins that appear to act as MAMPs in both Nicotiana benthamiana and Nicotiana tabacum (Chapter three). Furthermore, nuclear magnetic resonance was used to identify considerable changes in kauri leaf apoplastic wash fluid of approximately 17 metabolites, including sucrose and glucose, in response to P. agathidicida inoculation (Chapter four), thus suggesting a role for GH proteins in the hydrolysis of some of these metabolites. Finally, proteomic analysis of P. agathidicida culture filtrates via liquid chromatography-mass spectrometry (LC–MS) was used to validate the expression of predicted P. agathidicida proteins and to investigate the capacity of this method to identify candidate invasion patterns for future analysis. Chapter five established that LC–MS analysis of Phytophthora culture filtrate was an effective method for the identification of putative apoplastic invasion pattern candidates and confirmed the production of all six P. agathidicida GH12 cell death elicitors in culture. Collectively, this thesis has advanced our understanding of the molecular mechanisms underpinning the interaction of P. agathidicida with its host and has contributed to the identification of candidate apoplastic effectors.Item Kamahi decline in Tongariro National Park : a thesis presented for the degree of Doctor of Philosophy in Ecology at Massey University(Massey University, 1999) McBreen, Kim SuzanneForest dieback is a complex area of study that has led to the development of a number of theories or models which purport to explain it. These models are examined using the example of kamahi dieback in Tongariro National Park, There has long been concern over the health of kamahi in the Park and it is thought kamahi could be in a state of decline. A survey on three transects in the area of the Park where dieback is most apparent compared kamahi health to possible predisposing, triggering and hastening factors (the decline-disease theory of forest dieback) to determine their role in any dieback. Possums, pinhole borer, and Sporothrix fungus were highlighted in literature as likely triggering factors in kamahi dieback; an experiment examined their role: possums were excluded from trees, pinhole borer attack simulated, and healthy trees infected with Sporothrix, while unhealthy trees were treated with fungicide. To assess the effect of canopy health and vertebrate browsers on regeneration (regeneration is important in both the cohort senescence theory and the model of stand succession), another experiment was conducted using open and exclosure plots under healthy and thinning canopies. A second survey assessed the overall health of kamahi in the area, and compared site and tree factors to levels of dieback. The survey of an area with high apparent dieback found 14 % of kamahi stems were dead. There was some evidence that age predisposed stems to dieback, and Sporothrix was identified as accelerating stem death; no causal factor was determined. The experiment found no evidence that possums, pinhole borer, or Sporothrix were affecting the health of kamahi at this site. Sites under a thinning canopy in the regeneration study were much more variable in composition than sites under the healthy canopy; while sites in exclosure plots had higher densities of seedlings than sites that browsers had access to. Sites covered by the broad-scale survey contained very few unhealthy or dead kamahi trees, and none of the factors studied seemed to be impacting on the health of kamahi. There were more small (between 50 cm and 2 m) saplings present at sites with more dead kamahi trees. It was concluded that kamahi in Tongariro National Park is generally in a healthy state, and not undergoing decline; although localised dieback may be very high. Possums, pinhole borer and Sporothrix are not having a large effect on kamahi health in this area. Vertebrate browsers may be maintaining regeneration at a level below natural. Comparison between the theories of dieback, succession and the kamahi data indicate that the successional model may best explain the pattern of kamahi dieback. The models of dieback and succession can be inserted into an overriding model of dieback management, which will indicate the best path for investigating forest dieback.