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Subject: search.filters.subject.Endophyte

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    Viability of endophytic fungus in different perennial ryegrass (Lolium perenne) varieties kept in different storage conditions : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science at Massey University, Manawatū, New Zealand
    (Massey University, 2017) Kitson, Elizabeth Rose
    Epichloë endophytes form symbiotic relationships with cool-season grasses of the Pooideae family and are known to synthesise a range of bio-protective alkaloids. These alkaloids can provide the grass host with benefits for greater survival including; deterrence of herbivorous pests, increased persistence, better livestock health and protection from abiotic stressors. The commercialisation of novel endophytes is on the increase, and it is important to ensure the survival of the endophyte is maintained so their benefits can be realised. This study examined the effects of different storage conditions on the viability of three commercial novel endophytes (AR1, AR37 and NEA2/6) and one pre-commercial novel endophyte (815). The different storage conditions were the top of a warehouse, the bottom of a warehouse and a temperature and humidity controlled cool store to simulate current commercial seed storage environments. The viability of different endophytes decreases independently of grass seed germination (p = NS) however there are many factors influencing the endophyte survival. Over the one year storage period there were significant interactions between endophyte x ploidy (host), endophyte x location and endophyte x ploidy (host) x location. The pre-commercial endophyte, 815, had the largest reduction in viable endophyte when stored outside of the controlled cool store dropping 70 percentage points at the top of the warehouse, compared with AR37 (12 percentage points), AR1 (16 percentage points), and NEA2/6 (46 percentage points) (p<.001, LSD = 15.9). In the cool store there was no significant decrease in any of the treatments. As more novel endophyte/grass combinations are released for commercial sale it is important to test each for compatibility and performance post-storage. The results of this study recommend controlled low-temperature, low-humidity storage to maintain endophyte viability. Keywords: Endophyte, Epichloë, perennial ryegrass, Lolium perenne, storage
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    Neotyphodium lolii endophyte improves drought tolerance in perennial ryegrass (Lolium perenne. L) through broadly adjusting its metabolism : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy (PhD) in Microbiology and Genetics at Massey University, Manawatu, New Zealand
    (Massey University, 2014) Zhou, Yanfei
    Perennial ryegrass (Lolium perenne) is a widely used pasture grass that is frequently infected by Neotyphodium lolii endophyte. The presence of N. lolii enhances grass resistance to several biotic and abiotic stresses such as insect, herbivory and drought. Recent studies suggest the effect of N. lolii on ryegrass drought tolerance varies between grass genotypes. However, little is known about the molecular basis of how endophytes improve grass drought tolerance, why this effect varies among grass genotypes, or how the endophytes themselves respond to drought stress. This knowledge will not only increase our knowledge of beneficial plant-microbe interactions, but will also guide better use of endophytes, such as selection of specific endophyte - cultivar combinations for growth in arid areas. In this study, a real time PCR method that can accurately quantify N. lolii DNA concentration in grass tissue was developed for monitoring endophyte growth under drought. The effect of N. lolii on growth of 16 perennial ryegrass cultivars under drought was assessed, and a pair of endophyte-infected grasses showing distinct survival ability and performance under severe drought stress was selected. The transcriptome profiles of these two endophyte-infected grasses, as well as their clonal endophyte-free grasses, were analyzed using high-throughput RNA sequencing. The expression of endophyte and grass genes responsive to drought was analyzed simultaneously using different bioinformatic tools. The results demonstrated that N. lolii enhanced the growth of perennial ryegrass under drought, but the effect varied between cultivars. On the molecular level, endophytes living in both drought-tolerant and drought-sensitive grasses responded to drought in similar ways, including increased expression of endophyte genes related to scavenging of reactive oxygen species (ROS), DNA replication and the cell cycle, and also reduced expression of genes involved in alkaloids biosynthesis. The presence of endophytes also led to enhanced grass tolerance that was associated with broad adjustments in the plant metabolism. This included up regulation of grass genes involved in chloroplast maintenance and protection, osmotic adjustment and ROS scavenging capability. The extent of these endophyte-associated effects was greater in the drought tolerant grass genotype than in the drought sensitive genotype. This work highlights the role of fungal endophytes in grass drought stress tolerance and provides a comprehensive insight into the mechanisms involved.