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    UV-B Induced Flavonoids Contribute to Reduced Biotrophic Disease Susceptibility in Lettuce Seedlings.
    (Frontiers Media S.A., 2020-10-29) McLay ER; Pontaroli AC; Wargent JJ; Carvalho SM
    Biotrophic disease is one of the largest causes of decreased yield in agriculture. While exposure to ultraviolet B (UV-B) light (280-320 nm) has been previously observed to reduce plant susceptibility to disease, there is still a paucity of information regarding underlying biological mechanisms. In addition, recent advances in UV-LED technology raise the prospect of UV light treatments in agriculture which are practical and efficient. Here, we characterized the capability of UV-B LED pre-treatments to reduce susceptibility of a range of lettuce (Lactuca sativa) cultivars to downy mildew disease caused by the obligate biotroph Bremia lactucae. Innate cultivar susceptibility level did not seem to influence the benefit of a UV-B induced disease reduction with similar reductions as a percentage of the control observed (54-62% decrease in conidia count) across all susceptible cultivars. UV-B-induced reductions to conidia counts were sufficient to significantly reduce the infectivity of the diseased plant. Secondary infections caused by UV-B pre-treated plants exhibited yet further (67%) reduced disease severity. UV-B-induced flavonoids may in part mediate this reduced disease severity phenotype, as B. lactucae conidia counts of lettuce plants negatively correlated with flavonoid levels in a UV-B-dependent manner (r = -0.81). Liquid chromatography-mass spectrometry (LC-MS) was used to identify metabolic features which contribute to this correlation and, of these, quercetin 3-O-(6"-O-malonyl)-b-D-glucoside had the strongest negative correlation with B. lactucae conidia count (r = -0.68). When quercetin 3-O-(6"-O-malonyl)-b-D-glucoside was directly infiltrated into lettuce leaves, with those leaves subsequently infected, the B. lactucae conidia count was reduced (25-39%) in two susceptible lettuce cultivars. We conclude that UV-B induced phenolics, in particular quercetin flavonoids, may act as phytoanticipins to limit the establishment of biotrophic pathogens thus delaying or reducing their sporulation as measured by conidia count. These findings highlight the opportunity for UV-B morphogenesis to be exploited through the application of UV-LED technology, as part of the development of next-generation, sustainable disease control tools.
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    Development and prototyping of a solid state lighting product for architectural and accent applications : a thesis presented in fulfilment of the requirements for the degree of Master of Product Development in Technology at Massey University, School of Engineering & Technology, Albany campus, New Zealand
    (Massey University, 2009) Garnett, M. T.
    Far from being simply a necessary appliance to extend our day, artificial light has a great influence on human behaviour and wellbeing, perception of the surroundings and comfort. The energy needed for lighting is also a significant impact on our natural resources. For these two broad reasons lighting systems that improve the human visual and perceptual experience and reduce energy use are of widespread value. This work covers research into the application of LED technology as the next generation of mainstream lighting. It looks at the reasons why this technology is set to become the dominant way in which we light our lives, and the technical hurdles that are slowing this shift in lighting. It also presents the development, testing and prototyping of such an LED lighting product for use in the architectural market. This niche application is where LED lighting is currently most suited, due to the compactness, colour adjustability and lower colour rendering required. Establishing the technology here will help to gain consumer appreciation and acceptance of this beneficial and useful new paradigm in lighting. The design incorporates a shape that is pleasing to the eye with a simple oval profile. It was designed to be subtle and compact, blending into the ceiling as cleanly as possible. Practical testing on the finished prototype showed it to produce a wide range of colours and colour temperatures, while maintaining a safe LED temperature. The simplicity also makes the unit competitive in terms of cost.