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Subject: search.filters.subject.Microbial contamination

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    Contributions to food safety acceptance sampling plans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Statistics, Massey University, School of Mathematical and Computer Sciences
    (Massey University, 2023) Thevaraja, Mayooran
    An appropriate sampling inspection method is an essential tool for risk assessment in the food industry. A representative sampling approach will be helpful to reduce risk while minimising the sampling costs. Consequently, food manufacturers are employing efficient sampling approaches to assure food safety. In the food safety field, microbiological or other contamination often spreads unevenly across the production. Many factors are involved in the microbial risk assessment, such as (1) the amount of sample used for inspection, (2) what sampling methods were applied, (3) laboratory testing procedures, (4) physical sampling of materials from lots/batches of products and (5) the mixing of initially collected samples. This study focusses on improved sampling inspection approaches to reduce microbiological risk in food products. Part of this research also included developing open-source R packages to generate graphical displays for probabilistic risk assessment for practitioners. A single “wrapper” package is also provided to install all the newly developed packages in a single step.
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    Controlling biofilm development on ultrafiltration and reverse osmosis membranes used in dairy plants : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Food Technology at Massey University, Manawatu, New Zealand
    (Massey University, 2011) Tang, Xuemei; Tang, Xuemei
    This study aimed to develop improved cleaning strategies for controlling biofilms on the surfaces of membranes used in dairy ultrafiltration (UF) and reverse osmosis (RO) plants. Eleven UF / RO membrane modules from 7 different New Zealand dairy membrane processing plants were received after typical cleaning-in-place (CIP) procedures. Microorganisms were isolated from both the retentate and permeate sides of these membrane surfaces and from the liquids collected from a UF membrane plant. Also some foulants scraped from a RO membrane were tested. The routine CIP currently used in the dairy plants was not adequate to completely remove organic material, including microbial cells, proteins and carbohydrates from the membrane surfaces. These residues may influence the surface characteristics and interactions between microorganisms and membranes and thus affect biofilm formation. Thirteen isolates including both bacteria and yeast were identified using biochemical techniques. Klebsiella oxytoca were isolated from 3 different membrane plant sites. This is, so far as we know, the first report of K. oxytoca being isolated from dairy membrane surfaces. The ability of the 13 strains to attach to negatively charged polystyrene surfaces was tested using a microtitre plate assay. Three K. oxytoca strains demonstrated higher ability to adhere than the other strains, suggesting that these strains might play an important role in developing biofilms on dairy membrane surfaces. Two K. oxytoca strains (K. B006 from plant A, UF and K. TR002 from plant C, RO) that performed best in the microtitre screening assay with respect to attachment capabilities were chosen for the remainder of the study. The cell surface hydrophobicity of all isolates was determined using the microbial adhesion to hydrocarbon assay (MATH) and the cell surface charge was determined by measuring the surface zeta potential. These two characteristics did not show a clear relationship with the adherence of the isolated strains. However, it was found that bacterial attachment was enhanced in the presence of whey or mixed strains. A commercial biofilm reactor CBR 90 was modified for developing biofilms on membranes and investigating strategies for biofilm removal. Biofilms of single and dual K. oxytoca strains were developed under a continuous flow of whey. The saturated biofilm was approximately 8 log10 CFU cm-2. The results of our study suggested that the whey protein concentration, membrane type including membrane material (polyethersulfone (PES) and polyvinylidene fluoride (PVDF)), membrane age (used and new), bacterial strain and the interactions between different microorganisms are all significant factors for biofilm development on membrane surfaces. Three enzymatic cleaners and four sanitisers, including sodium hypochlorite (pH 6.5, 200 ppm free available chlorine (FAC)), Perform® (peracetic acid/hydrogen peroxide, 2% v/v), ozonated water (pH 7.0, 0.5 ppm free available ozone (FAO)) and anolyte of MIOX® electrolysed water (EW) (pH 6.8, 120 ppm FAC) were tested for their efficacies in killing culturable cells from biofilms formed by single or dual K. oxytoca strains on used PES membrane surfaces. With no sanitation applied, two of three enzymatic cleaners performed better than sodium hypochlorite (pH 10.8-11, 200 ppm FAC) commonly used for CIP of UF membranes in the dairy industry. The four sanitisers were used to treat the membranes after a CIP wash regime. The results indicated that if a dairy processor were to use a standard CIP on membrane systems, then a further flush with MIOX® EW anolyte would reduce residual attached microbial populations further. In addition, using protease followed by a sanitation (sodium hypochlorite, Perform® or anolyte of MIOX® EW) produced the best clean based on a greater than 2 log reduction in residual cells and left no culturable and viable cells at a detection limit of 0.1 log10 CFU cm-2.