Beyond BLASTing: How 3D structural prediction can support or refute BLAST results

Abstract

Biologists know that not all BLAST results are homologs and that trees lose information at deep times. We have therefore developed a pipeline to bring increased confidence to the BLAST result. We use structural prediction to support or refute the inclusion of protein sequences into multiple sequence alignments. Ancestral sequence reconstruction (ASR) together with structural prediction can be used to create seed sequences to help find remote homologs. The process can be used to help with annotation.