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Item Longitudinal study of herd udder hygiene and its association with clinical mastitis in pasture-based dairy cows(Elsevier Inc and the Federation of Animal Science Societies (FASS) Inc on behalf of the American Dairy Science Association, 2021-05) Rowe S; Tranter W; Laven RThe objectives of this exploratory study were to (1) describe the association between herd-level udder hygiene and clinical mastitis and (2) investigate how sample size and milking stage affect the accuracy and precision of herd udder hygiene assessments made at milking time. A prospective longitudinal study was conducted in a dairy herd in Northern Australia as part of a previously published clinical trial of premilking teat disinfection. Video footage from 35 afternoon milkings was used to conduct 12,544 udder hygiene scores from 504 cows during an 89-d period and measure udder hygiene of the herd (proportion of cows with udder hygiene ≥3 out of 4). Linear interpolation was used to estimate herd udder hygiene on the days that were not scored, such that a herd-level udder hygiene measure was available for all cow-days in the study. Clinical mastitis events occurring during the study period were detected and recorded by farm staff according to a standardized definition. The relationship between herd udder hygiene on each of 1, 2, and 3 d before each study day (d -1, -2, and -3, respectively) and clinical mastitis at the cow level on each study day (each in turn being set as d 0) was determined using multivariable generalized estimating equations (family = Poisson, link = log), with the unit of analysis being the cow-day, adjusting for potential confounders and the clustering within the data. In addition, sampling strategies were evaluated by simulating herd udder hygiene assessments using a subset of cows in the herd. Herd udder hygiene from d -1, -2, and -3 was positively associated with clinical mastitis on d 0 (incidence rate ratio = 1.4 per 10-point increase in the percentage of cows with poor udder hygiene). Sampling strategy simulation found that at least 80 cows needed to be scored to achieve sufficiently precise estimations of herd udder hygiene. Furthermore, cows scored later during milking were slightly more likely to have poor udder hygiene than those scored earlier (risk ratio = 1.02 for cows that were 10% later in the milking order). More research is needed to evaluate risk factors for poor udder hygiene and potential interventions in pasture-based dairy cows.Item Whole-Genome Sequencing and Virulome Analysis of Escherichia coli Isolated from New Zealand Environments of Contrasting Observed Land Use(American Society for Microbiology, 2022-05-10) Cookson AL; Marshall JC; Biggs PJ; Rogers LE; Collis RM; Devane M; Stott R; Wilkinson DA; Kamke J; Brightwell G; Elkins CAGeneric Escherichia coli is commonly used as an indicator of fecal contamination to assess water quality and human health risk. Where measured E. coli exceedances occur, the presence of other pathogenic microorganisms, such as Shiga toxin-producing E. coli (STEC), is assumed, but confirmatory data are lacking. Putative E. coli isolates (n = 709) were isolated from water, sediment, soil, periphyton, and feces samples (n = 189) from five sites representing native forest and agricultural environments. Ten E. coli isolates (1.41%) were stx2 positive, 19 (2.7%) were eae positive, and stx1-positive isolates were absent. At the sample level, stx2-positive E. coli (5 of 189, 2.6%) and eae-positive isolates (16 of 189, 8.5%) were rare. Using real-time PCR, these STEC-associated virulence factors were determined to be more prevalent in sample enrichments (stx1, 23.9%; stx2, 31.4%; eae, 53.7%) and positively correlated with generic E. coli isolate numbers (P < 0.05) determined using culture-based methods. Whole-genome sequencing (WGS) was undertaken on a subset of 238 isolates with assemblies representing seven E. coli phylogroups (A, B1, B2, C, D, E, and F), 22 Escherichia marmotae isolates, and 1 Escherichia ruysiae isolate. Virulence factors, including those from extraintestinal pathogenic E. coli, were extremely diverse in isolates from the different locations and were more common in phylogroup B2. Analysis of the virulome from WGS data permitted the identification of gene repertoires that may be involved in environmental fitness and broadly align with phylogroup. Although recovery of STEC isolates was low, our molecular data indicate that they are likely to be widely present in environmental samples containing diverse E. coli phylogroups. IMPORTANCE This study takes a systematic sampling approach to assess the public health risk of Escherichia coli recovered from freshwater sites within forest and farmland. The New Zealand landscape is dominated by livestock farming, and previous work has demonstrated that "recreational exposure to water" is a risk factor for human infection by Shiga toxin-producing Escherichia coli (STEC). Though STEC isolates were rarely isolated from water samples, STEC-associated virulence factors were identified more commonly from water sample culture enrichments and were associated with increased generic E. coli concentrations. Whole-genome sequencing data from both E. coli and newly described Escherichia spp. demonstrated the presence of virulence factors from E. coli pathotypes, including extraintestinal pathogenic E. coli. This has significance for understanding and interpreting the potential health risk from E. coli where water quality is poor and suggests a role of virulence factors in survival and persistence of E. coli and Escherichia spp.Item How can urban environments support dementia risk reduction? A qualitative study(John Wiley and Sons Ltd, 2021-12-28) Röhr S; Rodriguez FS; Siemensmeyer R; Müller F; Romero-Ortuno R; Riedel-Heller SGObjective Interventions to reduce the risk of cognitive decline and dementia largely focus on individual-level strategies. To maximize risk reduction, it is also necessary to consider the environment. With the majority of older people living in cities, we explored how urban environments could support risk reduction. Materials and Methods In our qualitative study, we conducted semi-structured interviews with community members aged ≥65 years and stakeholders, all living in Leipzig, Germany. Interview guides were informed by the framework on modifiable risk factors for dementia of the Lancet Commission on Dementia Prevention, Intervention, and Care. Interviews were audio-recorded, verbatim-transcribed, and thematically analysed. Results Community members (n = 10) were M = 73.7 (SD = 6.0) years old and 50% were women. Stakeholders (n = 10) were aged 39–72 years, and 70% were women. Stakeholders' fields included architecture, cultural/arts education, environmental sciences, geriatrics, health policy, information and technology, philosophy, psychology, public health, and urban sociology. Across interviews with both older individuals and stakeholders, three main themes were identified: (i) social participation and inclusion (emphasizing social contacts, social housing, intergenerationality, neighbourhood assistance, information and orientation, digital and technological literacy, lifelong learning, co-creation/co-design), (ii) proximity and accessibility (emphasizing proximity and reachability, mobility, affordability, access to health care, access to cultural events, public toilets), (iii) local recreation and wellbeing (emphasizing safety in traffic, security, cleanliness and environmental protection, urban greenery, climate change and heat waves, outdoor physical activity). Discussion The design of urban environments holds large potential to create favourable conditions for community-dwelling individuals to practice lifestyles that promote brain health. Public policy should involve community members in co-creating such environments.Item Weaving earth stories : practicing art sustainably and collaboratively with community and cow dung : an exegesis presented in partial fulfilment of the requirements for the postgraduate degree of Master of Fine Arts at Massey University, Wellington, New Zealand(Massey University, 2021) Tomar, ManjuThis exegesis is a record of my MFA journey, in a new country, in a phase of my practice that had already matured to a degree where I was clear what direction and conscious choices I wanted to pursue as an artist and a human on this planet. Before migrating from India to New Zealand, I had determined that cow dung was a material in abundance with many redeeming properties. While this material is extremely significant to me and the culture I grew up in, it is strangely regarded as a waste or a nuisance in NZ. A paradox emerged: New Zealand’s problem with cow manure offers its own solution using traditional craft knowledge that I brought with me. The thesis project developed over the two years of study and evolved from a solo studio artist making sculptures to a socially engaged community-based artist collaborating, leading, and facilitating the material’s capacity in the New Zealand context. The research project consists of a collection of early works that were representational and figurative in nature and focussed on presenting a personal narrative. Throughout these experiences, I was adapting to New Zealand weather, culture, soil, customs, and climate in relation to cow dung; I was finding my feet. A collection of three works followed that each demonstrate the evolution of my creative practice in this space of working with communities, ecological concepts, and cow dung.Item New Zealand's food system is unsustainable : a survey of the divergent attitudes of agriculture, environment and health sector professionals towards eating guidelines : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Nutrition and Dietetics at Massey University, Albany, New Zealand(Massey University, 2019) Jones, RebekahBackground: The United Nation’s (UN) Sustainable Development Goals (SDG) address the adverse health and environmental changes associated with changes in the food and nutrition system. In one of its many sustainable development initiatives, the Food and Agriculture Organisation of the UN (FAO) has called for sustainable diets, which align with SDG 2, Zero Hunger, and SDG 12, Sustainable Consumption and Production. The inclusion of sustainability characteristics in New Zealand’s (NZ) eating and activity guidelines (EAGs) would contribute to directly addressing the SDGs and lead to achieving sustainable diets. This study aimed to evaluate the agreement among sectoral professionals of including sustainability characteristics within the guidelines. Methods: Professionals within the agriculture, environment and health sectors were invited to complete an online survey to establish agreement with sustainability characteristics. Opinion and attitude questions were completed using a 5-item Likert scale. One-way ANOVA analyses were conducted to compare the level of agreement with the inclusion of sustainability statements of the three professional sector groups. A one-way ANCOVA analysis was undertaken to detect a difference in means of the sectoral levels of agreement whilst controlling for covariates. Post-hoc tests were used to determine where the significant differences in opinion lay between the sector groups. Results: Overall, 298 (65% female) respondents completed the survey from the agriculture (37%), environment (22%) and health (41%) sectors. Two-thirds (66%) of respondents were over 35 years and 90% had a tertiary education. Two-thirds (63%) of respondents disagreed with the statement that NZ’s current food system is sustainable; sector respondents from health (77%) and environment (78%) had greater disagreement than agriculture (35%) (P = 0.00). Overall, 77% of respondents agreed that sustainability characteristics should be included in guidelines; with greater agreement from health (90%) and environment (84%) versus agriculture (58%) (P = 0.00). Five sustainability characteristics received unanimously high levels of agreement (> 90%) for inclusion: dietary diversity (97%), sustainable seafood (90.8%), to limit processed foods (90.7%), reduced food waste (95.3%) and sustainable lifestyle behaviours (97.2%). Agreement for eight sustainability characteristics was higher for health and environment versus agricultural sector (P < 0.05). There was relatively lower level of agreement from all three sectors, particularly environmental (68.7%), to consume recommended serves of dairy products. Only 38.5% of all respondents agreed with the inclusion of “organic food produce”. Respondents who agreed with the inclusion of sustainability characteristics were more likely to be familiar with the EAGs and to agree NZs current food system is unsustainable. Conclusion: Professionals from the agriculture, environment and health sectors of New Zealand largely support the inclusion of sustainability characteristics within NZ’s EAGs. However, there are areas of divergence that need to be addressed for their successful development and implementation.