• Login
    View Item 
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Applications of cellulosic exchangers : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University

    Icon
    View/Open Full Text
    01_front.pdf (620.2Kb)
    02_whole.pdf (11.13Mb)
    Export to EndNote
    Abstract
    Two possible commercial applications for a new range of ion exchanger, based on regenerated cellulose were investigated. Equilibration studies showed that strongly basic (QAE) and strongly acidic (SP) derivatives can be equilibrated quicker and more easily than weakly basic (DEAE) and weakly acidic (CM) derivatives. This makes QAE and SP derivatives those of first choice, for use in commercial ion exchange processes. The new QA and DE Cellulose derivatives were investigated for their possible use in the commerical purification of rennet. It was found that they were unable to bind rennet with sufficient capacity, within it's pH stability limits, to be of any use in this process. This finding was surprising at first since these new ion exchangers showed improved BSA adsorption capacities over those currently employed for rennet purification. An explanation for this low capacity was proposed and verified with model studies on BSA adsorption. From these model studies it was also found that the new DE Cellulose has a more even distribution of charged groups resulting in sharper and more symmetrical peaks in the elution profiles of BSA, than those obtained from some DEAE celluloses commercially available. The new QA Cellulose was investigated for its possible use in the commercial extraction and purification of heparin, but was found to have insufficient density of charged groups to bind heparin at the high ionic strength used in some extraction processes. Several reaction schemes were devised and used to produce quaternary ammonium cellulose derivatives containing groups with two or three positively charged nitrogens as a means of increasing the charged density on the cellulose to match repeating negatively charged sulphate groups in heparin. The products obtained showed a dramatic increase in their binding strength for heparin but unfortunately there was a decrease in their capacity for heparin. None the less several potentially useful new cellulose derivatives for ion exchange chromatography can now be made.
    Date
    1983
    Author
    Elgar, David Francis
    Rights
    The Author
    Publisher
    Massey University
    URI
    http://hdl.handle.net/10179/11605
    Collections
    • Theses and Dissertations
    Metadata
    Show full item record

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1
     

     

    Tweets by @Massey_Research
    Information PagesContent PolicyDepositing content to MROCopyright and Access InformationDeposit LicenseDeposit License SummaryTheses FAQFile FormatsDoctoral Thesis Deposit

    Browse

    All of MROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1