• Login
    View Item 
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Characterisation and detection of dasheen mosaic potyvirus in Zantedeschia : a thesis presented in partial fulfilment of the requirements for the degree of Master of Horticultural Science in Plant Health at Massey University, Palmerston North, New Zealand

    Icon
    View/Open Full Text
    01_front.pdf (1.997Mb)
    02_whole.pdf (34.73Mb)
    Export to EndNote
    Abstract
    Four potyvirus isolates believed to be dasheen mosaic potyvirus, the most frequently occurring virus to infect members of the Araceae, were obtained from Caladium, Colocasia, Xanthosoma and Zantedeschia in world-wide locations. Properties of these isolates such as particle length, serological relatedness, electrophoretic mobility of coat proteins and genomic characteristics were compared. Serologically distinct strains of dasheen mosaic potyvirus were apparent amongst the isolates. The difference in the serological relationship was coupled with a variation in symptom expression. An isolate from Colocasia esculenta (L.) Schott was not serologically related to the other isolates. Further isolates from C. esculenta also exhibited no relationship. The modal length was different as well as the ability of complementary deoxyribonucleic acid, produced to the viral ribonucleic acid, to bind with some of the primers used in the polymerase chain reaction. This evidence led to the proposal that the isolate from C. esculenta was not dasheen mosaic potyvirus; this virus is tentatively named taro feathery mottle potyvirus. Cytoplasmic inclusion protein aggregates of dasheen mosaic potyvirus were purified from infected leaf tissue. SDS-polyacrylamide gel electrophoretic analysis of samples revealed a major band with an estimated molecular weight of 68,000 daltons. Such a band was absent from healthy tissue samples. The ATPase activity in samples from each purification step was determined by measuring the amount of [32P] released from the [λ-32P]ATP during incubation with the cytoplasmic inclusion protein. The level of ATPase activity in each sample showed a strong correlation with the amount of protein that was present. In a limited survey of commercial plantings twenty nine tubers grown for cutflower or tuber export were obtained from seven properties at different locations in New Zealand and grown on in a greenhouse. Each plant was indexed for virus infection. Electron microscopy revealed that plants from three of the properties contained 720nm flexuous rods. Samples from all but two plants tested positive to a potyvirus group antiserum using the enzyme-linked immunosorbent assay. The remaining two plants tested positive in microprecipitin and rapid immune electron microscopy tests to an antiserum prepared to a member of the carlavirus group. Particles from these plants were mechanically transmitted to Nicotiana tabacum 'Havana'. Rod-shaped particles of 300nm were observed in plants from four properties and tested positive to tobacco mosaic tobamovirus antiserum using a microprecipitin test. While inoculations to herbaceous indicators resulted in no symptoms, 300nm particles were observed in samples from the indicator plants. Tomato spotted wilt tospovirus, potato X potexvirus and cucumber mosaic cucumovirus, reported to infect Zantedeschia spp, were not detected.
    Date
    1995
    Author
    Matthews, Christine Grace
    Rights
    The Author
    Publisher
    Massey University
    URI
    http://hdl.handle.net/10179/12271
    Collections
    • Theses and Dissertations
    Metadata
    Show full item record

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1
     

     

    Tweets by @Massey_Research
    Information PagesContent PolicyDepositing content to MROCopyright and Access InformationDeposit LicenseDeposit License SummaryTheses FAQFile FormatsDoctoral Thesis Deposit

    Browse

    All of MROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1