An investigation of cellulose ion exchangers : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University, New Zealand

Abstract

The aim of this thesis was to prepare a range of polysaccharide ion exchangers and to explore their potential for use in chromatographic methods and their ability to remove protein from solution. The ion exchangers were prepared from regenerated cellulose cross linked with epichlorohydrin and hydroxyalkylated with propylene oxide. The preparations of the DEAE -, CM- and SP- derivatives were investigated and the products shown to be chemically stable, to allow high flow rates and to have excellent capacities for adsorbing protein. Practical applications of these ion exchangers were demonstrated. The DEAE-derivative was used for the chromatographic fractionation of serum proteins and an enzyme purification. The CM- and SP- derivatives were found to be useful for removing protein from whey. The conditions, such as pretreatment of whey and pH, were investigated to find the conditions necessary for good protein uptake and from the results a new process was developed for efficient recovery of protein from whey by ion exchange.