Show simple item record

dc.contributor.authorPetersen, Melvin John
dc.date.accessioned2018-02-15T19:33:36Z
dc.date.available2018-02-15T19:33:36Z
dc.date.issued1978
dc.identifier.urihttp://hdl.handle.net/10179/12771
dc.description.abstractThe aim of this thesis was to prepare a range of polysaccharide ion exchangers and to explore their potential for use in chromatographic methods and their ability to remove protein from solution. The ion exchangers were prepared from regenerated cellulose cross linked with epichlorohydrin and hydroxyalkylated with propylene oxide. The preparations of the DEAE -, CM- and SP- derivatives were investigated and the products shown to be chemically stable, to allow high flow rates and to have excellent capacities for adsorbing protein. Practical applications of these ion exchangers were demonstrated. The DEAE-derivative was used for the chromatographic fractionation of serum proteins and an enzyme purification. The CM- and SP- derivatives were found to be useful for removing protein from whey. The conditions, such as pretreatment of whey and pH, were investigated to find the conditions necessary for good protein uptake and from the results a new process was developed for efficient recovery of protein from whey by ion exchange.en_US
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectIon exchangeen_US
dc.subjectChromatographyen_US
dc.titleAn investigation of cellulose ion exchangers : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University, New Zealanden_US
dc.typeThesisen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M. Sc.)en_US


Files in this item

Icon
Icon

This item appears in the following Collection(s)

Show simple item record