• Login
    View Item 
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Sugar and organic acid utilisation in Pachysolen tannophilus and Pichia stipitis : a thesis presented in partial fulfilment of the requirements for the degree in Master of Science in Genetics at Massey University

    Icon
    View/Open Full Text
    01_front.pdf (804.7Kb)
    02_whole.pdf (3.038Mb)
    Export to EndNote
    Abstract
    Wild-type strains of the K+ yeasts Pachysolen tannophilus and Pichia stipitis were assessed for their ability to utilise tricarboxylic acid (TCA) cycle intermediates and to ferment glucose and xylose. These two species of yeast were mutagenised using ultraviolet (UV) radiation. The survivors were screened on an indicator plate for mutants with altered rates of malate utilisation, in the presence of glucose. The Pichia stipitis wild-type strain was UV mutagenised, and mutants resistant to the lethal analog of glucose, 2-deoxyglucose, were selected. These mutants, plus several mutants of Pachysolen tannophilus isolated in an earlier study, were partially characterised by growth on a variety of solid media. The mutants were also grown on indicator media containing xylose or glucose plus one TCA cycle intermediate: citrate, succinate, fumarate or L-malate. Experiments with the indicator media served both as a method of characterisation of the mutants, and as a method of testing the utility of the indicator plate. The behavior of these mutants when grown under semi-anaerobic conditions in mixtures of glucose and TCA cycle intermediates was investigated. The wild-type Pachysolen tannophilus co-utilised malate and glucose, whereas in the wild-type Pichia stipitis, malate utilisation was repressed by glucose. Some mutants of both species showed increased utilisation of malate (and succinate, where tested). In Pachysolen tannophilus, this behavior correlated with loss of all three hexose-ATP-kinase enzymes, and in Pichia stipitis the behavior correlated with the loss of any hexose­ ATP-kinase activity.
    Date
    1991
    Author
    Harrod, Christopher John
    Rights
    The Author
    Publisher
    Massey University
    URI
    http://hdl.handle.net/10179/14568
    Collections
    • Theses and Dissertations
    Metadata
    Show full item record

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1
     

     

    Tweets by @Massey_Research
    Information PagesContent PolicyDepositing content to MROCopyright and Access InformationDeposit LicenseDeposit License SummaryTheses FAQFile FormatsDoctoral Thesis Deposit

    Browse

    All of MROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1