Filamentous phage derived biological nanorods : development of a novel display system : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University, Manawatū, New Zealand. EMBARGOED until 5 September 2024.
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Date
2018
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Massey University
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Abstract
The Ff filamentous bacteriophage are filament-like bacterial viruses approximately 900 nm in length. The F-pilus-specific filamentous phage are resistant to heat, pH-extremes, and detergents in combination with their structural properties and amenability to DNA recombinant engineering has enabled their extensive use in modern biotechnology. However, the use of Ff-phage in vaccines and other such biological uses is controversial due to their ability to replicate in gut Escherichia coli, and the possibility of mobilisation and horizontal gene transfer of antibiotic resistance-encoding genes among the gut bacteria. As such, the novel system was established to create short, stable particles that cannot replicate, called NanoZap particles. However, this system has the disadvantage of often producing multiple-length particles, rather than the desired single-length particles. In this thesis several variations upon the original pNanoZap vector were created and tested to obtain monodisperse unit-length particles.--Shortened abstract
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Embargoed until 5 September 2024
Keywords
Bacteriophages, Genetics, Nanoparticles, Drug resistance in microorganisms, Plasmids