• Login
    View Item 
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Metabolism and translocation of linamarin in cassava (Manihot Esculenta Crantz) : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, New Zealand

    Icon
    View/Open Full Text
    02_whole.pdf (6.164Mb)
    01_front.pdf (1.449Mb)
    Export to EndNote
    Abstract
    The metabolism of linamarin in cassava (Manihot esculenta Crantz) has been investigated. Information on the biosynthetic pathway, synthetic sites, translocation and turnover of the cyanoglucoside has been obtained by precursor administrations to various parts of cassava plants grown under partially controlled conditions in the glasshouse. Three volatile 14C-labelled precursors of linamarin isobutyronitrile, isobutyraldoxime and 2-hydroxyisobutyronitrile were prepared, purified and administered to cassava leaves by a new technique in which the leaves were allowed to take up precursor vapour in an enclosed glass chamber. The incorporation of these precursors, and of L-valine administered by solution uptake, was consistent with a pattern of linamarin biosynthesis in cassava involving the reaction sequence through valine, isobutyraldoxime, isobutyronitrile and 2-hydroxyisobutyronitrile established for other plants. The solution administration of L-[U- 14C] valine to various organs of the plant indicated that the leaves and the shoot apex synthesised linamarin more efficiently than the woody stem and the roots and tubers. More detailed investigations of leaf biosynthesis showed much higher incorporation of 14C-valine into linamarin by the petioles and midribs (45-62% 14C incorporation by petioles and 20% by midribs) than the leaf blades (2%). There was no direct relationship between endogenous linamarin content (which was higher in the blades than the petioles) and the apparent ability to synthesise linamarin from exogenous valine. However, the low ability of the blade tissue to incorporate valine into linamarin could be due to more active competing pathways removing the exogenously administered valine. In further investigations with tuber peels and the edible cores, similar competing pathways have been implicated for an apparently low biosynthetic efficiency of linamarin. The translocation of linamarin was demonstrated by specifically labelling 14C-linamarin in attached leaves with 2-hydroxy[1-14C] isobutyronitrile vapour and following the change in labelled linamarin content in the leaf and the distribution of linamarin to other parts of the plant. In both non-tuberous and tuberous plants there was a rapid loss of 14C-linamarin due to translocation from the fully expanded leaves up to 69 hours after synthesis. However a residual component of the 14C-linamarin (25-37% of that initially synthesised) remained in the leaves. A compartmentation of synthesised linamarin in cassava leaf tissues into a readily mobile and partially immobile fraction would account for these observations. In senescing leaves a continuous loss of both 14C-labelled and endogenous linamarin occurred leaving almost no residual component although this was attributed to both translocation and turnover. Translocated linamarin was distributed to all parts of the plant but the general pattern of translocate flow differed between non-tuberous and tuberous plants. An apical direction of linamarin distribution existed in the non-tuberous plants while tuber-directed linamarin translocation prevailed in the tuberous plants. Leaf senescence apparently enhances linamarin translocation to the tubers. There was little turnover of freshly synthesised 14C-linamarin in detached leaves and tuber tissues over a period of 1 to 3 days. However the low recoveries of 14C-linamarin in the whole plant translocation experiments suggest that active turnover may be occurring during translocation or in certain sink tissues.
    Date
    1977
    Author
    Bediako, Badu Michael Kofi
    Rights
    The Author
    Publisher
    Massey University
    URI
    http://hdl.handle.net/10179/3627
    Collections
    • Theses and Dissertations
    Metadata
    Show full item record

    Copyright © Massey University
    Contact Us | Send Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1
     

     

    Tweets by @Massey_Research
    Information PagesContent PolicyDepositing content to MROCopyright and Access InformationDeposit LicenseDeposit License SummaryTheses FAQFile FormatsDoctoral Thesis Deposit

    Browse

    All of MROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © Massey University
    Contact Us | Send Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1