• Login
    View Item 
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    •   Home
    • Massey Documents by Type
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The aetiology and pathogenesis of avian inclusion body hepatitis : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Virology, Massey University

    Icon
    View/Open Full Text
    02_whole.pdf (4.799Mb)
    01_front.pdf (600.9Kb)
    Export to EndNote
    Abstract
    Naturally occurring inclusion body hepatitis (IBH) in broiler flocks in New Zealand, and the experimental disease were characterized by a sudden onset of illness resulting in up to 30% mortality and severe liver damage associated with the formation of intranuclear inclusion bodies in the hepatocytes. Other features were anaemia and atrophy of the bursa and thymus associated with lymphoid depletion. Serotype 8 avian adenoviruses (AAVs) were isolated from several affected broiler and one breeder flock. High titres of virus neutralizing (VN) antibodies were demonstrated in flocks which had recovered from the disease. By restriction endonuclease fingerprint analysis, two of the New Zealand isolates were found to be similar to each other and to the reference strain HVI, but markedly different from three Australian isolates of the same serotype. Fatal disease resembling IBH was reproduced in 30% of broiler chickens following oral administration of one of the local isolates. Immunosuppression was demonstrated in both natural and experimental infections. An enzyme-linked immunosorbent assay and an immunocytochemical technique were developed for the detection and quantification of adenoviral antigens in various chicken tissues. Both techniques detected less than 100 mean tissue culture infective doses per gram of infected tissue and a group-specific antigen common to the 12 serotypes of AAV. A study of the pathogenesis of IBH infection was conducted following oral administration of AAV. Virus first multiplied to a high titre in the intestinal organs and passed into the blood by way of the lymphatics. Viral antigens were subsequently detected in phagocytic cells in the liver and then in the hepatocytes. Extensive replication resulted in severe liver damage, with release of virus into the blood stream and spread to other organs. Recovery was associated with the appearance of VN antibody from 7 days post inoculation. Viral antigens were detected by ELISA directly in yolk and albumin of eggs derived from 50-60-week-old breeder flocks, although all birds had high titres of VN antibody in their blood. The inclusion bodies found in hepatocytes were characterized antigenically and ultrastructurally.
    Date
    1990
    Author
    Saifuddin, Mohammed
    Rights
    The Author
    Publisher
    Massey University
    URI
    http://hdl.handle.net/10179/4651
    Collections
    • Theses and Dissertations
    Metadata
    Show full item record

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1
     

     

    Tweets by @Massey_Research
    Information PagesContent PolicyDepositing content to MROCopyright and Access InformationDeposit LicenseDeposit License SummaryTheses FAQFile FormatsDoctoral Thesis Deposit

    Browse

    All of MROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © Massey University
    | Contact Us | Feedback | Copyright Take Down Request | Massey University Privacy Statement
    DSpace software copyright © Duraspace
    v5.7-2020.1-beta1