Use of semi-anaemic piglets to measure iron bioavailability of meat and meat fractions: a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Nutritional Science at Massey University, Albany-Palmerston North, New Zealand
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Date
2003
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Massey University
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Abstract
Iron deficiency is a major nutritional problem. According to the World Health Organization (WHO), there are approximately 3.7 billion people in the world with iron deficiency (WHO, 2000). Red meat is known to enhance iron absorption due in part to the presence of a meat factor. Domestic pigs (Sus domesticus) have been utilised as models for humans in many medical and nutritional studies. The first experiment reported here used 20 4-week-old piglets to compare the bioavailability of iron in diets containing meat (ME), a water-soluble extract of meat (SA), a water-insoluble fraction of meat (SR), and a meat-free control diet (CO). Bioavailability of iron was assessed on the basis of changes in iron-related blood parameters over a 4-week feeding trial. Iron retention in haemoglobin, red blood cell counts (RBC), haemoglobin levels (HGB), haematocrit (HCT), and mean corpuscular volumes (MCV) for ME, SR, and SA was significantly higher (p < 0.05) than for CO indicating that all meat fractions enhanced the bioavailability of iron. For some blood parameters the iron status of group SA was significantly lower (p<0.05) than for groups ME or SR. It is concluded that the meat-factor is primarily present in the water-insoluble fraction of beef. In the second experiment nine four weeks old of age female pigs were allocated into three groups of diet treatments, i.e. E200, which contained 48 g/kg live weight0.75 of meat extrinsically labelled with Fe57; 1200 and 1300 , which contained 48 g/kg liveweight0.75 and 69 g/kg liveweight0.75 of meat intrinsically labelled with Fe57 The isotope labelled diets were fed only on day 0. Afterwards all pigs received the same weaner diet. The pigs were also injected by 58Fe via intra venous. E200, 1 200, and 1 300 were not significantly different (p > 0.05) in growth parameters (i.e. average daily gain and average daily feed intake) and in the blood parameters (i.e. white blood cells and RBC, HGB, HCT, MCV, the mean corpuscular hemoglobin and the total iron body in the blood circulation) and iron absorptions. The findings indicate that the different labeling method and different meat levels gave same results.
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Keywords
Iron bioavailability, Iron in the body, Swine, Piglets, Pigs, Feed utilisation efficiency