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dc.contributor.authorPungulani, Lawrent Lino Michael
dc.date.accessioned2015-05-25T21:12:02Z
dc.date.available2015-05-25T21:12:02Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10179/6620
dc.description.abstractThe shortage of improved cowpea (Vigna unguiculata (L.) Walp) varieties and increased frequency of droughts in Malawi have created a need to identify drought tolerant genotypes with desirable agronomic and utility characteristics. This research evaluated local germplasm maintained by the Malawi Plant Genetic Resources Centre (MPGRC), as an initial step towards the identification of genotypes with drought tolerance. Eco-geographic characterisation revealed diverse ecologies among the different germplasm collected. These genotypes were subsequently assessed for drought tolerance in a glasshouse study. All genotypes which tolerated low moisture conditions in the glasshouse originated from areas with high rainfall and low temperatures suggesting that extreme environmental conditions and/or human mediated actions interfered with adaptation processes. Furthermore, the eco-geographic characterisation identified germplasm gaps which need to be filled by either collection or repatriation of germplasm from international genebanks. The establishment of on-farm conservation in areas with low rainfall and high temperature such as Chikwawa and Nsanje districts may enhance adaptation of cowpea to drought conditions. Genotypes 479, 601, 645, 2226 and 3254 fully recovered from moisture stress, while 2232 started wilting within one week of drought stress initiation in the first glasshouse experiment. The genotypes which recovered from moisture stress showed low scores for wilting scales, low leaf wilting index (LWI), high relative water content, high scores for stem greenness and high levels for re-growth. In addition, the first glasshouse experiment resulted in the development of a leaf wilting index, which has been identified as an easily used method for scoring wilting, compared to common wilting scales. In a subsequent glasshouse experiment, all the genotypes which fully recovered from moisture stress showed high relative water content during the period of stress, but showed differences in other physiological traits. For example, genotypes 479, 601, 645 and 2226 had reduced stomatal conductance, transpiration rate and net photosynthesis, while 3254 maintained high scores for the three traits from the initial stage of moisture stress. Although 2232 showed a high transpiration rate and stomatal conductance, its net photosynthesis was significantly reduced, compared to all the other genotypes, after the third week of stress. The differences in physiological traits among genotypes indicated that 3254 has drought tolerance; 479, 601, 645 and 2226 avoid drought while 2232 is drought susceptible. The field performance of these six genotypes and two released varieties (Sudan 1 and IT82E16) was assessed in field trials in Malawi at Baka, Bvumbwe, Chitala, Chitedze and Kasinthula. Results from field trials revealed significant variation for reproductive, yield and seed characteristics. Sudan 1, IT82E16, 409 and 601 matured in less than 65 days after planting; 3254 took 70 days and 645, 2226 and 2232 took more than 85 days. Genotype 3254 consistently gave high yields at sites with low rainfall and high temperatures compared to 2232 which yielded poorly at the same sites. The eight genotypes showed variation in seed size with genotype 2226 producing large seeds (>20g/100 seeds) at all sites. The seed size of 2232 was significantly lower than 2226 at sites with low rainfall and high temperatures. The field performance of these genotypes reflects the physiological responses observed in the glasshouse, confirming the drought response categories of the genotypes. The agreement between glasshouse experiments and the field trial suggests there is intrinsic value in the locally adapted germplasm maintained by the Malawi Plant Genetic Resources Centre. Among the genotypes tested in the field, farmers selected 479 for early maturity; 2226 and 2232 for high leaf biomass; 3254 for high pod load; 2226 and 2232 for large seeds; Sudan 1 for small seeds; and 601, IT82E16 and Sudan 1 for smooth seed texture. Genotype 3254 was ranked poorly at all the sites due to rough seed texture. Genotypes for potential use in improving production of cowpea in drought prone areas were identified. In the absence of released drought tolerant varieties, it is recommended that genotypes with drought avoidance characteristics be promoted in areas with mild droughts, while 3254 with its typical drought tolerance may be suitable for areas with intense droughts. However, the rough seed texture of 3254 may limit its usefulness due to its poor ranking by farmers at all sites. Priorities for future cowpea in Malawi include investigating inheritance of drought tolerance in cowpea.en_US
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectCowpeaen_US
dc.subjectVigna unguiculataen_US
dc.subjectCowpea, Malawien_US
dc.subjectDrought toleranceen_US
dc.titleExploring the genetic potential of locally adapted germplasm for drought tolerance : a case for cowpea (Vigna unguiculata (L.)) Walp) from Malawi : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Plant Science, Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealanden_US
dc.typeThesisen_US
thesis.degree.disciplinePlant Scienceen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhilosopy (Ph.D.)en_US


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