Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain

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dc.citation.issue3
dc.citation.volume241
dc.contributor.authorPalmer R
dc.contributor.authorCornuault VRG
dc.contributor.authorMarcus SE
dc.contributor.authorKnox JP
dc.contributor.authorShewry PR
dc.contributor.authorTosi P
dc.date.available2015-03-01
dc.date.issued2015-03-01
dc.description.abstractCell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre. Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain. Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes. Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling. A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls. Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species. Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form. A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain. Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues. In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
dc.description.confidentialfalse
dc.format.extent669 - 685
dc.identifier.citationPlanta, 2015, 241 (3), pp. 669 - 685
dc.identifier.doi10.1007/s00425-014-2201-4
dc.identifier.elements-id403211
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0032-0935
dc.languageEnglish
dc.publisherSpringer Verlag
dc.relation.isPartOfPlanta
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328131/#__ffn_sectitle
dc.rights© The Author(s) 2014 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited
dc.subjectcell wall
dc.subjectgrain development
dc.subjectimmunodetection
dc.subjectrice grain
dc.subjectwheat grain
dc.subject.anzsrc0607 Plant Biology
dc.subject.anzsrc0703 Crop and Pasture Production
dc.titleComparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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