Genomic analysis of canine pneumoviruses and canine respiratory coronavirus from New Zealand.

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dc.citation.issue4
dc.citation.volume72
dc.contributor.authorDunowska M
dc.contributor.authorMore GD
dc.contributor.authorBiggs PJ
dc.contributor.authorCave NJ
dc.coverage.spatialEngland
dc.date.accessioned2024-07-18T21:30:07Z
dc.date.available2024-07-18T21:30:07Z
dc.date.issued2024-07-01
dc.description.abstractAIMS: To isolate canine respiratory coronavirus (CRCoV) and canine pneumovirus (CnPnV) in cell culture and to compare partial genomic sequences of CRCoV and CnPnV from New Zealand with those from other countries. METHODS: Oropharyngeal swab samples from dogs affected by canine infectious respiratory disease syndrome that were positive for CnPnV (nā€‰=ā€‰15) or CRCoV (nā€‰=ā€‰1) by virus-specific reverse transcriptase quantitative PCR (RT-qPCR) in a previous study comprised the starting material. Virus isolation was performed in HRT-18 cells for CRCoV and RAW 264.7 and Vero cells for CnPnV. The entire sequence of CnPnV G protein (1,266 nucleotides) and most (8,063/9,707 nucleotides) of the 3' region of CRCoV that codes for 10 structural and accessory proteins were amplified and sequenced. The sequences were analysed and compared with other sequences available in GenBank using standard molecular tools including phylogenetic analysis. RESULTS: Virus isolation was unsuccessful for both CRCoV and CnPnV. Pneumovirus G protein was amplified from 3/15 (20%) samples that were positive for CnPnV RNA by RT-qPCR. Two of these (NZ-048 and NZ-049) were 100% identical to each other, and 90.9% identical to the third one (NZ-007). Based on phylogenetic analysis of the G protein gene, CnPnV NZ-048 and NZ-049 clustered with sequences from the USA, Thailand and Italy in group A, and CnPnV NZ-007 clustered with sequences from the USA in group B. The characteristics of the predicted genes (length, position) and their putative protein products (size, predicted structure, presence of N- and O-glycosylation sites) of the New Zealand CRCoV sequence were consistent with those reported previously, except for the region located between open reading frame (ORF)3 (coding for S protein) and ORF6 (coding for E protein). The New Zealand virus was predicted to encode 5.9 kDa, 27 kDa and 12.7 kDa proteins, which differed from the putative coding capacity of this region reported for CRCoV from other countries. CONCLUSIONS: This report represents the first characterisation of partial genomic sequences of CRCoV and CnPnV from New Zealand. Our results suggest that the population of CnPnV circulating in New Zealand is not homogeneous, and that the viruses from two clades described overseas are also present here. Limited conclusions can be made based on only one CRCoV sequence, but the putative differences in the coding capacity of New Zealand CRCoV support the previously reported variability of this region. The reasons for such variability and its biological implications need to be further elucidated.
dc.description.confidentialfalse
dc.edition.edition2024
dc.format.pagination191-200
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38650102
dc.identifier.citationDunowska M, More GD, Biggs PJ, Cave NJ. (2024). Genomic analysis of canine pneumoviruses and canine respiratory coronavirus from New Zealand.. N Z Vet J. 72. 4. (pp. 191-200).
dc.identifier.doi10.1080/00480169.2024.2339845
dc.identifier.eissn1176-0710
dc.identifier.elements-typejournal-article
dc.identifier.issn0048-0169
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70235
dc.languageeng
dc.publisherTaylor and Francis Group
dc.publisher.urihttps://www.tandfonline.com/doi/full/10.1080/00480169.2024.2339845
dc.relation.isPartOfN Z Vet J
dc.rights(c) 2024 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCanine respiratory coronavirus
dc.subjectNew Zealand canine respiratory disease
dc.subjectcanine pneumovirus
dc.subjectphylogeny
dc.subjectsequence analysis
dc.subjectAnimals
dc.subjectDogs
dc.subjectNew Zealand
dc.subjectCoronavirus, Canine
dc.subjectDog Diseases
dc.subjectPneumovirus
dc.subjectGenome, Viral
dc.subjectPhylogeny
dc.subjectCoronavirus Infections
dc.subjectVero Cells
dc.subjectChlorocebus aethiops
dc.titleGenomic analysis of canine pneumoviruses and canine respiratory coronavirus from New Zealand.
dc.typeJournal article
pubs.elements-id488550
pubs.organisational-groupOther
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