Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time

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dc.citation.issue1
dc.citation.volume227
dc.contributor.authorBinny RN
dc.contributor.authorPriest P
dc.contributor.authorFrench NP
dc.contributor.authorParry M
dc.contributor.authorLustig A
dc.contributor.authorHendy SC
dc.contributor.authorMaclaren OJ
dc.contributor.authorRidings KM
dc.contributor.authorSteyn N
dc.contributor.authorVattiato G
dc.contributor.authorPlank MJ
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-22T23:50:36Z
dc.date.accessioned2024-07-25T06:38:49Z
dc.date.available2022-07-25
dc.date.available2024-01-22T23:50:36Z
dc.date.available2024-07-25T06:38:49Z
dc.date.issued2023-01-01
dc.description.abstractBACKGROUND: Reverse transcription polymerase chain reaction (RT-PCR) tests are the gold standard for detecting recent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Reverse transcription PCR sensitivity varies over the course of an individual's infection, related to changes in viral load. Differences in testing methods, and individual-level variables such as age, may also affect sensitivity. METHODS: Using data from New Zealand, we estimate the time-varying sensitivity of SARS-CoV-2 RT-PCR under varying temporal, biological, and demographic factors. RESULTS: Sensitivity peaks 4-5 days postinfection at 92.7% (91.4%-94.0%) and remains over 88% between 5 and 14 days postinfection. After the peak, sensitivity declined more rapidly in vaccinated cases compared with unvaccinated, females compared with males, those aged under 40 compared with over 40s, and Pacific peoples compared with other ethnicities. CONCLUSIONS: Reverse transcription PCR remains a sensitive technique and has been an effective tool in New Zealand's border and postborder measures to control coronavirus disease 2019. Our results inform model parameters and decisions concerning routine testing frequency.
dc.description.confidentialfalse
dc.format.pagination9-17
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35876500
dc.identifier.citationBinny RN, Priest P, French NP, Parry M, Lustig A, Hendy SC, Maclaren OJ, Ridings KM, Steyn N, Vattiato G, Plank MJ. (2022). Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time.. J Infect Dis. 227. 1. (pp. 9-17).
dc.identifier.doi10.1093/infdis/jiac317
dc.identifier.eissn1537-6613
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-1899
dc.identifier.pii6649627
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70603
dc.languageeng
dc.publisherOxford University Press on behalf of Infectious Diseases Society of America
dc.publisher.urihttps://academic.oup.com/jid/article/227/1/9/6649627
dc.relation.isPartOfJ Infect Dis
dc.rights(c) The author/sen
dc.rights.licenseCC BY-NC-ND 4.0en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectCOVID-19
dc.subjectSARS-CoV-2
dc.subjectreversetranscriptionpolymerase chain reaction
dc.subjectsurveillance
dc.subjecttest sensitivity
dc.subjectMale
dc.subjectFemale
dc.subjectHumans
dc.subjectAged
dc.subjectSARS-CoV-2
dc.subjectCOVID-19
dc.subjectCOVID-19 Testing
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectReverse Transcription
dc.subjectClinical Laboratory Techniques
dc.subjectSensitivity and Specificity
dc.subjectReal-Time Polymerase Chain Reaction
dc.titleSensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time
dc.typeJournal article
pubs.elements-id455221
pubs.organisational-groupOther
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