Isolation, antibacterial screening, and identification of bioactive cave dwelling bacteria in Fiji.

Simple item page
dc.citation.volume13
dc.contributor.authorPipite A
dc.contributor.authorLockhart PJ
dc.contributor.authorMcLenachan PA
dc.contributor.authorChristi K
dc.contributor.authorKumar D
dc.contributor.authorPrasad S
dc.contributor.authorSubramani R
dc.contributor.editorMoyer CL
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-07-22T03:05:39Z
dc.date.available2024-07-22T03:05:39Z
dc.date.issued2022-12-20
dc.description.abstractBacteria are well known producers of bioactive secondary metabolites, including some of the most effective antibiotics in use today. While the caves of Oceania are still largely under-explored, they form oligotrophic and extreme environments that are a promising source for identifying novel species of bacteria with biologically active compounds. By using selective media that mimicked a cave environment, and pretreatments that suppressed the growth of fast-growing bacteria, we have cultured genetically diverse bacteria from a limestone cave in Fiji. Partial 16S rRNA gene sequences from isolates were determined and compared with 16S rRNA gene sequences in EzBioCloud and SILVA data bases. Fifty-five isolates purified from culture had Actinomycete-like morphologies and these were investigated for antibacterial activity. Initial screening using a cross streak test with pathogenic bacteria indicated that 34 of the isolates had antibacterial properties. The best matches for the isolates are bacteria with potential uses in the manufacture of antibiotics and pesticides, in bioremediation of toxic waste, in biomining, in producing bioplastics, and in plant growth promotion. Nineteen bacteria were confirmed as Actinomycetes. Thirteen were from the genus Streptomyces and six from genera considered to be rare Actinomycetes from Pseudonocardia, Kocuria, Micromonospora, Nonomuraea. Ten isolates were Firmicutes from the genera Bacillus, Lysinbacillus, Psychrobacillus and Fontibacillus. Two were Proteobacteria from the genera Mesorhizobium and Cupriavidus. Our findings identify a potentially rich source of microbes for applications in biotechnologies.
dc.description.confidentialfalse
dc.edition.editionDecember 2022
dc.format.pagination1012867-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36605510
dc.identifier.citationPipite A, Lockhart PJ, McLenachan PA, Christi K, Kumar D, Prasad S, Subramani R. (2022). Isolation, antibacterial screening, and identification of bioactive cave dwelling bacteria in Fiji.. Front Microbiol. 13. (pp. 1012867-).
dc.identifier.doi10.3389/fmicb.2022.1012867
dc.identifier.eissn1664-302X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-302X
dc.identifier.number1012867
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70265
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1012867/full
dc.relation.isPartOfFront Microbiol
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject16S rRNA gene
dc.subjectFiji
dc.subjectactinomycetes
dc.subjectantibiotics
dc.subjectbioactive bacteria
dc.subjectbioactivity screening
dc.subjectcaves
dc.subjectselective isolation
dc.titleIsolation, antibacterial screening, and identification of bioactive cave dwelling bacteria in Fiji.
dc.typeJournal article
pubs.elements-id458862
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Evidence.pdf
Size:
1.44 MB
Format:
Adobe Portable Document Format
Description:
Data_Sheet_1_Isolation, antibacterial screening, and identification of bioactive cave dwelling bacteria in Fiji.pdf
Download
Thumbnail Image
Name:
Published version.pdf
Size:
3.93 MB
Format:
Adobe Portable Document Format
Description:
458862 PDF.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles