Validation of a Relative Centrifugal Force method for the enumeration and detection of Campylobacter from chicken carcass rinsates

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dc.citation.volume236
dc.contributor.authorKingsbury JM
dc.contributor.authorMidwinter A
dc.contributor.authorMills J
dc.contributor.authorEnglefield M
dc.contributor.authorBiggs R
dc.contributor.authorPerchec Merien A-M
dc.contributor.authorDermer N
dc.contributor.authorSoni A
dc.contributor.authorBlakemore M
dc.date.accessioned2025-08-26T01:36:45Z
dc.date.available2025-08-26T01:36:45Z
dc.date.issued2025-09-01
dc.description.abstractCampylobacteriosis is the most frequently notified foodborne disease in New Zealand and poultry is the predominant infection source. New Zealand monitors Campylobacter present in poultry carcass rinsates under the National Microbiological Database (NMD) programme. To better monitor Campylobacter control improvements, a more sensitive method is required that can enumerate rinsates with lower Campylobacter numbers. This study developed a modification of the current NMD method involving adding a relative centrifugal force (RCF) step for concentrating Campylobacter from poultry carcass rinsates. Centrifugation for 30 min significantly improved Campylobacter recovery compared with 15 min (p < 0.001), but there were no differences between RCFs of 3500, 4000 and 4430 x g (p = 0.992). RCF and NMD method performances were compared in a single laboratory validation study that used different inoculation levels of twelve Campylobacter strains, including poultry isolates. Campylobacter was detected from more samples (p < 0.001) using the RCF method (93 of 126; 73.8 %) than the NMD method (65 of 126; 51.6 %). The RCF method had a seven-fold lower detection limit (28 colony forming units (CFU)/400 ml) than the NMD method (200 CFU/400 ml). The detection limit accounted for an observed 70.3 % of the inoculated CFU captured within the centrifuged pellet. Campylobacter was also detected from significantly more (p < 0.001) commercial chicken rinsate samples tested by poultry industry laboratories using the RCF method (257 of 863; 29.8 %) than the NMD method (114 of 863; 13.2 %). Taken together, results support the RCF method as a modification of the NMD method to enumerate lower numbers of Campylobacter in rinsates.
dc.description.confidentialfalse
dc.edition.editionSeptember 2025
dc.identifier.citationKingsbury JM, Midwinter A, Mills J, Englefield M, Biggs R, Perchec Merien AM, Dermer N, Soni A, Blakemore M. (2025). Validation of a Relative Centrifugal Force method for the enumeration and detection of Campylobacter from chicken carcass rinsates. Journal of Microbiological Methods. 236.
dc.identifier.doi10.1016/j.mimet.2025.107207
dc.identifier.eissn1872-8359
dc.identifier.elements-typejournal-article
dc.identifier.issn0167-7012
dc.identifier.number107207
dc.identifier.piiS016770122500123X
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/73418
dc.languageEnglish
dc.publisherElsevier B V
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S016770122500123X
dc.relation.isPartOfJournal of Microbiological Methods
dc.rights(c) 2025 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEnumeration method
dc.subjectCentrifugation
dc.subjectPoultry meat
dc.subjectHygiene indicator
dc.subjectRegulatory testing
dc.titleValidation of a Relative Centrifugal Force method for the enumeration and detection of Campylobacter from chicken carcass rinsates
dc.typeJournal article
pubs.elements-id502728
pubs.organisational-groupOther
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