Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses

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dc.citation.issue6
dc.citation.volume13
dc.contributor.authorPanizzi L
dc.contributor.authorDittmer KE
dc.contributor.authorVignes M
dc.contributor.authorDoucet JS
dc.contributor.authorGedye K
dc.contributor.authorWaterland MR
dc.contributor.authorRogers CW
dc.contributor.authorSano H
dc.contributor.authorMcIlwraith CW
dc.contributor.authorRiley CB
dc.contributor.editorZucca E
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-05-14T03:18:35Z
dc.date.available2024-05-14T03:18:35Z
dc.date.issued2023-03-14
dc.description.abstractBiomarkers for osteoarthritis (OA) in horses have been extensively investigated, but translation into clinical use has been limited due to cost, limited sensitivity, and practicality. Identifying novel biomarkers that overcome these limitations could facilitate early diagnosis and therapy. This study aimed to compare the concentrations of synovial fluid (SF) and plasma cell-free DNA (cfDNA) over time in control horses with those with induced carpal OA. Following an established model, unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder were sham-operated controls. Synovial fluid and plasma samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63, and cfDNA concentrations were determined using fluorometry. The SF cfDNA concentrations were significantly higher for OA joints than for sham-operated joints on Days 28 (median 1430 μg/L and 631 μg/L, respectively, p = 0.017) and 63 (median 1537 μg/L and 606 μg/L, respectively, p = 0.021). There were no significant differences in plasma cfDNA between the OA and the sham groups after induction of carpal OA. Plasma cfDNA measurement is not sufficiently sensitive for diagnostic purposes in this induced model of OA. Synovial fluid cfDNA measurement may be used as a biomarker to monitor early disease progression in horses with OA.
dc.description.confidentialfalse
dc.edition.editionMarch-2 2023
dc.format.pagination1053-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36978592
dc.identifier.citationPanizzi L, Dittmer KE, Vignes M, Doucet JS, Gedye K, Waterland MR, Rogers CW, Sano H, McIlwraith CW, Riley CB. (2023). Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses.. Animals (Basel). 13. 6. (pp. 1053-).
dc.identifier.doi10.3390/ani13061053
dc.identifier.eissn2076-2615
dc.identifier.elements-typejournal-article
dc.identifier.issn2076-2615
dc.identifier.number1053
dc.identifier.piiani13061053
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/69555
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2076-2615/13/6/1053
dc.relation.isPartOfAnimals (Basel)
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbiomarker
dc.subjectcarpus
dc.subjectcell-free DNA
dc.subjecthorse
dc.subjectosteoarthritis
dc.subjectplasma
dc.subjectsynovial fluid
dc.titlePlasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses
dc.typeJournal article
pubs.elements-id460758
pubs.organisational-groupOther
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