Validity and reliability of Raman spectroscopy for carotenoid assessment in cattle skin

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dc.citation.volume27
dc.contributor.authorMehta M
dc.contributor.authorNaffa R
dc.contributor.authorZhang W
dc.contributor.authorSchreurs NM
dc.contributor.authorWaterland M
dc.contributor.authorCooper S
dc.contributor.authorHolmes G
dc.coverage.spatialNetherlands
dc.date.accessioned2024-01-11T22:02:32Z
dc.date.accessioned2024-07-25T06:33:39Z
dc.date.available2021-06-02
dc.date.available2024-01-11T22:02:32Z
dc.date.available2024-07-25T06:33:39Z
dc.date.issued2021-09
dc.description.abstractCarotenoids are powerful antioxidants capable of helping to protect the skin from the damaging effects of exposure to sun by reducing the free radicals in skin produced by exposure to ultraviolet radiation, and they may also have a physical protective effect in human skin. Since carotenoids are lipophilic molecules which can be ingested with the diet, they can accumulate in significant quantities in the skin. Several studies on humans have been conducted to evaluate the protective function of carotenoids against various diseases, but there is very limited published information available to understand the mechanism of carotenoid bioavailability in animals. The current study was conducted to investigate the skin carotenoid level (SCL) in two cattle skin sets - weaners with an unknown feeding regime and New Generation Beef (NGB) cattle with monitored feed at three different ages. Rapid analytical and sensitive Raman spectroscopy has been shown to be of interest as a powerful technique for the detection of carotenoids in cattle skin due to the strong resonance enhancement with 532 nm laser excitation. The spectral difference of both types of skin were measured and quantified using univariate and linear discriminant analysis. SCL was higher in NGB cattle than weaners and there is a perfect classification accuracy between weaners and NGB cattle skin using carotenoid markers as a basis. Further work carried out on carotenoid rich NGB cattle skin of 8, 12 and 24 months of age identified an increasing trend in SCL with age. The present work validated the ability of Raman spectroscopy to determine the skin carotenoid level in cattle by comparing it with established HPLC methods. There is an excellent correlation of R2 = 0.96 between the two methods that could serve as a model for future application for larger population studies.
dc.description.confidentialfalse
dc.edition.editionSeptember 2021
dc.format.pagination101036-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34141905
dc.identifier.citationMehta M, Naffa R, Zhang W, Schreurs NM, Waterland M, Cooper S, Holmes G. (2021). Validity and reliability of Raman spectroscopy for carotenoid assessment in cattle skin.. Biochem Biophys Rep. 27. (pp. 101036-).
dc.identifier.doi10.1016/j.bbrep.2021.101036
dc.identifier.eissn2405-5808
dc.identifier.elements-typejournal-article
dc.identifier.issn2405-5808
dc.identifier.number101036
dc.identifier.piiS2405-5808(21)00130-8
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70416
dc.languageeng
dc.publisherElsevier BV
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2405580821001308
dc.relation.isPartOfBiochem Biophys Rep
dc.rights(c) 2021 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectANOVA, analysis of variance
dc.subjectAntioxidants
dc.subjectCarotenoids
dc.subjectHPLC, High Performance Liquid Chromatography
dc.subjectHigh Performance Liquid Chromatography
dc.subjectLASRA, New Zealand Leather and Shoe Research Association
dc.subjectLDA, linear discriminant analysis
dc.subjectNGB, new generation beef
dc.subjectPCA, principal component analysis
dc.subjectRaman spectroscopy
dc.subjectSCL, skin carotenoid level
dc.subjectUV, ultraviolet
dc.titleValidity and reliability of Raman spectroscopy for carotenoid assessment in cattle skin
dc.typeJournal article
pubs.elements-id446126
pubs.organisational-groupOther
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