Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems.

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dc.citation.volume246
dc.contributor.authorCornuault VRG
dc.contributor.authorPose S
dc.contributor.authorKnox JP
dc.date.available2018-04-25
dc.date.issued2018-04-25
dc.description.abstractThe matrix polysaccharides of plant cell walls are diverse and variable sets of polymers influencing cell wall, tissue and organ properties. Focusing on the relatively simple parenchyma tissues of four fruits – tomato, aubergine, strawberry and apple – we have dissected cell wall matrix polysaccharide contents using sequential solubilisation and antibody-based approaches with a focus on pectic homogalacturonan (HG) and rhamnogalacturonan-I (RG-I). Epitope detection in association with anion-exchange chromatography analysis indicates that in all cases solubilized polymers include spectra of HG molecules with unesterified regions that are separable from methylesterified HG domains. In highly soluble fractions, RG-I domains exist in both HG-associated and non-HG-associated forms. Soluble xyloglucan and pectin-associated xyloglucan components were detected in all fruits. Aubergine glycans contain abundant heteroxylan epitopes, some of which are associated with both pectin and xyloglucan. These profiles of polysaccharide heterogeneity provide a basis for future studies of more complex cell and tissue systems.
dc.description.confidentialfalse
dc.format.extent275 - 285
dc.identifier.citationFood Chemistry, 2018, 246 pp. 275 - 285
dc.identifier.doi10.1016/j.foodchem.2017.11.025.
dc.identifier.elements-id403103
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0308-8146
dc.languageEnglish
dc.publisherElsevier
dc.relation.isPartOfFood Chemistry
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0308814617318356?via=ihub
dc.rightsOpen Access funded by Biotechnology and Biological Sciences Research Council under a Creative Commons license.
dc.subjectpectic polysaccharides
dc.subjectfruit cell wall
dc.subjecthomogalacturonan
dc.subjectrhamnogalacturonan I
dc.subjectheteroxylan
dc.subjectSolanaceae
dc.subjectRosaceae
dc.titleDisentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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