Antigen-specific cytotoxic T lymphocytes target airway CD103+ and CD11b+ dendritic cells to suppress allergic inflammation

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dc.citation.volume9
dc.contributor.authorDaniels NJ
dc.contributor.authorHyde E
dc.contributor.authorGhosh S
dc.contributor.authorSeo K
dc.contributor.authorPrice KM
dc.contributor.authorHoshino K
dc.contributor.authorKaisho T
dc.contributor.authorOkada T
dc.contributor.authorRonchese F
dc.date.available2016-01
dc.date.available2015-05-19
dc.date.issued2016-01
dc.description.abstractAllergic airway inflammation is driven by the recognition of inhaled allergen by T helper type 2 (Th2) cells in the airway and lung. Allergen-specific cytotoxic T lymphocytes (CTLs) can strongly reduce airway inflammation, however, the mechanism of their inhibitory activity is not fully defined. We used mouse models to show that allergen-specific CTLs reduced early cytokine production by Th2 cells in lung, and their subsequent accumulation and production of interleukin (IL)-4 and IL-13. In addition, treatment with specific CTLs also increased the proportion of caspase+ dendritic cells (DCs) in mediastinal lymph node (MLN), and decreased the numbers of CD103+ and CD11b+ DCs in the lung. This decrease required expression of the cytotoxic mediator perforin in CTLs and of the appropriate MHC-antigen ligand on DCs, suggesting that direct CTL-DC contact was necessary. Lastly, lung imaging experiments revealed that in airway-challenged mice XCR1-GFP+ DCs, corresponding to the CD103+ DC subset, and XCR1-GFP− CD11c+ cells, which include CD11b+ DCs and alveolar macrophages, both clustered in the areas surrounding the small airways and were closely associated with allergen-specific CTLs. Thus, allergen-specific CTLs reduce allergic airway inflammation by depleting CD103+ and CD11b+ DC populations in the lung, and may constitute a mechanism through which allergic immune responses are regulated.
dc.description.confidentialfalse
dc.format.extent229 - 239 (11)
dc.identifier.citationMucosal Immunology, 2016, 9 pp. 229 - 239 (11)
dc.identifier.doi10.1038/mi.2015.55
dc.identifier.elements-id280296
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1935-3456
dc.publisherNature Publishing Group
dc.relation.isPartOfMucosal Immunology
dc.relation.replaceshttp://hdl.handle.net/123456789/2969
dc.relation.replaces123456789/2969
dc.relation.urihttp://www.nature.com/mi/journal/v9/n1/full/mi201555a.html
dc.subjectImmunology
dc.subjectDendritic cells
dc.subjectFlow cytometry
dc.subjectRNA
dc.subjectCytotoxicity
dc.subjectCTL
dc.subjectT lymphocytes
dc.subjectCD103+
dc.subjectCD11b+
dc.subjectantigen
dc.subjectantibody
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc11 Medical and Health Sciences
dc.titleAntigen-specific cytotoxic T lymphocytes target airway CD103+ and CD11b+ dendritic cells to suppress allergic inflammation
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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