Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept.

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dc.citation.volume12
dc.contributor.authorOn SLW
dc.contributor.authorZhang Y
dc.contributor.authorGehring A
dc.contributor.authorPatsekin V
dc.contributor.authorChelikani V
dc.contributor.authorFlint S
dc.contributor.authorWang H
dc.contributor.authorBillington C
dc.contributor.authorFletcher GC
dc.contributor.authorLindsay J
dc.contributor.authorRobinson JP
dc.contributor.editorFusco V
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-10-24T00:56:01Z
dc.date.accessioned2023-10-25T20:49:30Z
dc.date.available2021-02-17
dc.date.available2023-10-24T00:56:01Z
dc.date.available2023-10-25T20:49:30Z
dc.date.issued2021-02-17
dc.date.updated2023-10-16T20:19:04Z
dc.description.abstractIsolation of the pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis from foods typically rely on slow (10-21 day) "cold enrichment" protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five Yersinia species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of Y. enterocolitica and Y. pseudotuberculosis. The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring Yersinia strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae.
dc.description.confidentialfalse
dc.edition.editionFebruary 2021
dc.format.extent641801-
dc.identifierARTN 641801
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/33679677
dc.identifier.citationOn SLW, Zhang Y, Gehring A, Patsekin V, Chelikani V, Flint S, Wang H, Billington C, Fletcher GC, Lindsay J, Robinson JP. (2021). Elastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept.. Front Microbiol. 12. (pp. 641801-).
dc.identifier.doi10.3389/fmicb.2021.641801
dc.identifier.eissn1664-302X
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10179/20384
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2021.641801/full
dc.relation.isPartOfFront Microbiol
dc.rightsCC BY 4.0en_US
dc.rights(c) 2021 The Author/sen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectYersinia
dc.subjectdetection
dc.subjectdiagnostics
dc.subjectelastic light scatter
dc.subjectfood
dc.titleElastic Light Scatter Pattern Analysis for the Expedited Detection of Yersinia Species in Pork Mince: Proof of Concept.
dc.typeJournal article
pubs.elements-id441126
pubs.organisational-groupOther
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